Shared Resources

Glycotechnology Core – Link to Glycotech webpage

Services Currently Available:

  • Sugar composition analysis (HPAEC-PAD/GC-MS)
  • Linkage analysis (GC-MS)
  • Sialic acid analysis (HPAEC-PAD/FL/GC-MS)
  • Structural analysis of N- and O-Glycan (HPAEC-PAD-FL/MALDI/LC-MS)
  • Structural analysis of Bacterial Polysaccharides (LPS/CPS/EPS/Peptido-glycan)
  • Glycosaminoglycan (GAG) analysis (HPLC/LC-MS)
  • Detection of Sugar Nucleotides and modified Nucleosides by HPLC-UV/LC-MS
  • Detection and quantification of Fatty acids by GC-MS
  • Detection and quantification of Sulfate and Phosphate by HPAEC-Conductivity detector

Upcoming Services:

  • Capillary Electrophoresis
  • Purification of glycans for comparative study

Costs: Fee for service and via collaboration

Availability: Immediate and Upcoming

Chemical Synthesis of Oligosaccharides Component – Link to webpage

Well-defined complex carbohydrates cannot be easily be obtained from natural sources. Although challenging, chemical or chemo-enzymatic synthesis can address this deficiency.

Specific Goals:

  • Chemical synthesis of glycosaminoglycan (GAG) standards for quantitative analysis
  • Chemical synthesis of libraries of HS oligosaccharides for SAR studies
  • Chemical synthesis of sialosides for studies of Siglecs

Costs: Agreement of collaboration

Availability: Immediate

Histology – Link to Webpage

The overall goal is to enhance and facilitate the research of the individual research units by providing the following histological services and training to program members:

Routine assays

  • Tissue sectioning
  • Preparation of paraffin and frozen sections and training as needed
  • Provide routine hematoxylin and eosin stains

Training and assistance with harvesting organs and processing of tissues

  • Harvesting of mouse tissues using optimal methods, with freezing of organs, tissues and cells
  • Fixing, orienting, processing and embedding in paraffin, organs, tissues and ce

Special stains, lectin histochemistry and immunohistology

  • Standard and specialized histochemical staining and training as needed
  • Lectin histochemistry with controls and inhibitors
  • Immunohistological staining, of frozen/paraffin sections with enzyme/fluorochrome-labels
  • Antigen retrieval and signal amplification methods
  • Assays for apoptosis or for proliferating cells

Training and assistance with microscopic analysis and interpretation of histological studies

  • Advice on experimental design of histological studies
  • Help with microscopic analysis, histological evaluation, interpretation, and photo-documentation

Costs: Fee for service and Agreement of collaboration

Availability: Immediate

Hematology and Clinical Chemistry – Link to Webpage

Screening and specific assays to identify phenotypic abnormalities in hematopoietic and metabolic functions of genetically altered mice. Depending on results, additional specific assays are performed in consultation with project leaders. Depending on specific aims of individual project, other directed studies can be performed. Core E will also assist in training project’s members in learning techniques.

Routine assays

  • Hematology screen: Complete blood count with white cell subsets (differential count) and microscopic examination of peripheral blood smears for cellular morphology.
  • Cytokine screen: a panel of acute phase and inflammatory cytokines will be measured which includes IL1A, IL1B, IL2, IL4, IL6, IL10, IL12, IL17A, IFNγ, TNFα, G-CSF, and GM-CSF.
  • Metabolic screen: serum electrolytes, total protein, IgG immunoglobulin, liver enzymes (AST, ALT, ALP), and lipids (LDL, HDL, TG) to evaluate the liver, pancreas, and kidney functions and metabolic status.
  • Hemostasis screen: prothrombin time (PT) and activated partial thromboblastin time (aPTT).
  • Urinary screen: pH, specific gravity, protein content, glucose, ketones, and nitrite

Special Assays

  • Coagulation, anticoagulatiion and fibrinolytic plasma levels, tail bleeding time, von Willebrand antigen levels, platelet function studies, in vivo clearance studies.

Costs: Fee for service and Agreement of collaboration

Availability: Immediate