"Strategically Coordinate Efficient Use and Sharing of Expertise and Technologies…"

Shared Resources

Biosynthesis and Function of Lactosaminyl Glycans in Hematopoiesis

Glycoanalytic Component (Dr. Vernon Reinhold)
Comprehensive sequence profiling for protein and lipid glycans on cells

Adhesion Assay Component (Dr. Robert Sackstein)
Perform cell adhesion assays to measure binding of activity of integrins (e.g., VLA-4, VLA-5, LFA-1), of CD44 to hyaluronic acid, and of selectin ligands. This component will also perform shear-based adhesion assays to assess mediators of binding interactions under shear conditions.

Ex Vivo Glycan Engineering Component (Dr. Robert Sackstein)
Utilize newly developed glycosyltransferase reagents and attendant buffers expressly formulated to prevent cell toxicity, to perform stereospecific glycan modification of the surfaces of living cells.

In Vitro Glycosyltransferase and Glycosidase Assay Component (Dr. Joseph Lau)
Perform measurement of glycosyltransferase and glycosidase activities in cellular homogenates.

Availability: Immediate via collaboration
Details and Contact Information
BWH PEG website

Hyaluronan Matrices in Vascular Pathologies

Glycosaminoglycan (GAG) analysis using Fluorophore-Assisted-Carbohydrate-Electrophoresis (FACE) technology (Dr. Mark Lauer)
Availability: Immediate and upcoming via collaboration
Details and Contact Information

Glyconjugates & Cardiovascular Disease – CardioPEG

Proteomics and Glycomics (Dr. Jennifer Van Eyk & Dr. Hui Zhang)
Provide protocols, and analysis using state-of-the-art mass spectrometry (MS) and other proteomic technologies, including identification of glycoproteins, glycosylation sites information, quantitative glycosylation site occupancy, and glycan screening and determination.

Nuclear Magnetic Resonance (NMR) (Dr. Alan Bush)
If samples allow, NMR is the best way to analyze glycan or glycoconjugate structure.

Chemistry Core (Dr. Kevin Yarema)
Specializes in monosaccharide analogues used in “metabolic glycoengeering” technology but is also pursuing inhibitors of glycosyltransferases and developing methods to improve the pharmacological properties of sugar based drugs to facilitate their in vivo use and clinical translation.

O-GlcNAc Core (Dr. Gerald Hart & Dr. Natasha Zachara)
Provide the necessary reagents, specialized equipment, and expertise to study the O-GlcNAc modification in cardiac models, as well as providing a national resource for studying O-GlcNAcylation in physiological models of interest to the NHLBI.

Myoctye Isolation and Physiology Core (Dr. David Kass & Dr. Nazareno Paolocci)
Provides a centralized mechanism to generate cellular and organ-level analysis of cardiovascular function, and to supply individual projects with isolated myocytes for additional molecular/protein analysis.

Availability: Immediate and upcoming via collaboration
Details and Contact Information
CardioPEG website

Glycobiology of Inflammatory Lung Diseases – LIDPEG

Synthesis of sialic acid-containing ligands using chemo-enzymatic syntheses (Dr. Corwin Nycholat).

Compounds also available to the scientific community for nominal charge to cover costs associated with producing the compounds and shipping.

Availability: Immediate via fee for service
Details and Contact Information
Link to LIDPEG website

Glycan Modulation of Inflammatory Responses

Glycan Analysis & Synthesis (Dr. Biswa Choudhury)
Sugar composition analysis (HPAEC-PAD/GC-MS)
Linkage analysis (GC-MS)
Sialic acid analysis (HPAEC-PAD/FL/GC-MS)
Structural analysis of N- and O-Glycan (HPAEC-PAD-FL/MALDI/LC-MS)
Structural analysis of Bacterial Polysaccharides (LPS/CPS/EPS/Peptido-glycan)
Glycosaminoglycan (GAG) analysis (HPLC/LC-MS)
Detection of Sugar Nucleotides and modified Nucleosides by HPLC-UV/LC-MS
Detection and quantification of Fatty acids by GC-MS
Detection and quantification of Sulfate and Phosphate by HPAEC-Conductivity detector
Chemical Synthesis of Oligosaccharides Component (Dr. Geert Jan Boons)
Chemical synthesis of glycosaminoglycan (GAG) standards for quantitative analysis
Chemical synthesis of libraries of HS oligosaccharides for SAR studies
Chemical synthesis of sialosides for studies of Siglecs

Histology (Dr. Nissi Varki)
The overall goal is to enhance and facilitate the research of the individual research units by providing the following histological services and training to program members

Hematology and Clincial Chemistry (Dr. Dzung Le)
Screening and specific assays to identify phenotypic abnormalities in hematopoietic and metabolic functions of genetically altered mice.

Availability: Immediate via fee for service and collaboration
Details and Contact Information

The Chemistry and Biology of Heparan Sulfate

Synthetic Resource (Dr. Kuby Balagurunathan)
Reagents available include: a) Computationally designed heparan sulfates (HS) (μg levels); b) A library of synthetic xylosides for in vitro synthesis of glycoaminoglycans (GAGs).

Computational Resources (Dr. Umesh Desai)
Technologies available include: a) Computational screening algorithm for designing GAG structures with potential therapeutic benefits; b) Ready-made virtual library of 46,586 natural HS hexasaccharide structures; c) Software licenses for operating computational screening algorithm.

GAG Analysis Resources (Drs. Umesh Desai & Kuby Balagurunathan)
Resources available include: a) nESI-qTOF-MS spectrometer for structural analysis of GAGs; b) Capillary electrophoresis for studying how GAGs interact with proteins of physiological or pharmacological relevance; c) UPLC – TDQ MS spectrometer for structural analysis of GAGs and their complexes with proteins.

Biophysics Resources (Drs. Umesh Desai & Dr. Krishna Rajarathnam)
Technologies available include: a) Applied Photophysics stopped-flow fluorimeter for the study of kinetics of GAG interacting with proteins; b) Atomic Force Microscopy for study of interactions of GAGs at a single molecule level; c) High resolution NMR for solution structures of GAG – protein complexes; d) Surface Plasmon Resonance for measuring affinities of GAG for proteins; e) Isothermal calorimetry for thermodynamics of GAG binding to proteins; f) analytical ultracentrifugation for stoichiometry of GAG – protein complexes

Chemokine Resources (Dr. Krishna Rajarathnam)
Reagents available include: a) Human interleukin – 8; b) Human neutrophil activating peptide – 8; c) Mouse KC
Reagents within 1 year include: a) 13C and 15N – labeled chemokines for NMR structural studies; b) monomer and dimer variants of chemokines for biophysical, structural, and functional studies; c) chemokine mutants – for structural and functional studies.

Coagulation Function Resources (Dr. David Cooper)
Technology available include: a) In vitro assays for testing effect on coagulation dysfunction; b) Ex vivo model of organ hemoperfusion; c) In vivo nonhuman primate model of organ transplantation.

Availability: Immediate via fee for service and collaboration
Details and Contact Information