Publications

Tumorigenic potential is restored during differentiation in fusion-reprogrammed cancer cells

2017-04-06

Yao J, Zhang L, Hu L, Guo B, Hu X, Borjigin U, Wei Z, Chen Y, Lv M, Lau JT, Wang X, Li G, Hu YP. Cell Death Dis. 2016 Jul 28;7(7):e2314. doi: 10.1038/cddis.2016.189. PMID: 27468690 Abstract Detailed understanding of the mechanistic steps underlying tumor initiation and malignant progression...

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Glycoengineering of E-Selectin Ligands by Intracellular versus Extracellular Fucosylation Differentially Affects Osteotropism of Human Mesenchymal Stem Cells

2017-04-06

Dykstra B, Lee J, Mortensen LJ, Yu H, Wu ZL, Lin CP, Rossi DJ, Sackstein R. Stem Cells. 2016 Oct;34(10):2501-2511. doi: 10.1002/stem.2435. PMID: 27335219 Abstract Human mesenchymal stem cells (MSCs) hold great promise in cellular therapeutics for skeletal diseases but lack expression of E-selectin ligands that direct homing of...

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Platelet clearance by the hepatic Ashwell-Morrell receptor: mechanisms and biological significance

2017-04-06

Hoffmeister KM, Falet H. Thromb Res. 2016 May;141 Suppl 2:S68-72. doi: 10.1016/S0049-3848(16)30370-X. PMID: 27207430 Abstract The daily production of billions of platelets must be regulated to avoid spontaneous bleeding or arterial occlusion and organ damage. Complex mechanisms control platelet production and clearance in physiological and pathological conditions. This review...

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Glycans and the platelet life cycle

2017-04-06

Li R, Hoffmeister KM, Falet H. Platelets. 2016 Sep;27(6):505-11. doi: 10.3109/09537104.2016.1171304. PMID: 27135356 Abstract Platelet numbers are intricately regulated to avoid spontaneous bleeding or arterial occlusion and organ damage. The growth factor thrombopoietin (TPO) drives platelet biogenesis by inducing megakaryocyte production. A recent study in mice identified a feedback...

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Fulfilling Koch's postulates in glycoscience: HCELL, GPS and translational glycobiology

2017-04-06

Sackstein R. Glycobiology. 2016 Jun;26(6):560-70. doi: 10.1093/glycob/cww026. Review. PMID: 26933169 Abstract Glycoscience-based research that is performed expressly to address medical necessity and improve patient outcomes is called "translational glycobiology". In the 19th century, Robert Koch proposed a set of postulates to rigorously establish causality in microbial pathogenesis, and these...

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Regulating billions of blood platelets: glycans and beyond

2017-04-06

Grozovsky R, Giannini S, Falet H, Hoffmeister KM. Blood. 2015 Oct 15;126(16):1877-84. doi: 10.1182/blood-2015-01-569129. Review. PMID: 26330242 Abstract The human body produces and removes 10(11) platelets daily to maintain a normal steady state platelet count. Platelet production must be regulated to avoid spontaneous bleeding or arterial occlusion and organ...

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2015 proceedings of the National Heart, Lung, and Blood Institute's State of the Science in Transfusion Medicine symposium.

2017-04-06

Spitalnik SL, Triulzi D, Devine DV, Dzik WH, Eder AF, Gernsheimer T, Josephson CD, Kor DJ, Luban NL, Roubinian NH, Mondoro T, Welniak LA, Zou S, Glynn S; State of the Science in Transfusion Medicine Working Groups.. Transfusion. 2015 Sep;55(9):2282-90. doi: 10.1111/trf.13250. PMID: 26260861 Abstract On March 25 and...

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Desialylation is a mechanism of Fc-independent platelet clearance and a therapeutic target in immune thrombocytopenia

2017-04-06

Li J, van der Wal DE, Zhu G, Xu M, Yougbare I, Ma L, Vadasz B, Carrim N, Grozovsky R, Ruan M, Zhu L, Zeng Q, Tao L, Zhai ZM, Peng J, Hou M, Leytin V, Freedman J, Hoffmeister KM, Ni H. Nat Commun. 2015 Jul...

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Novel mechanisms of platelet clearance and thrombopoietin regulation

2017-04-06

Grozovsky R, Giannini S, Falet H, Hoffmeister KM. Curr Opin Hematol. 2015 Sep;22(5):445-51. doi: 10.1097/MOH.0000000000000170. Review. PMID: 26154508 Abstract PURPOSE OF REVIEW: The human body produces and removes 10 platelets daily to maintain a normal steady-state platelet count. Platelet production must be tightly regulated to avoid spontaneous bleeding or arterial...

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Cell surface glycan engineering of neural stem cells augments neurotropism and improves recovery in a murine model of multiple scleros

2017-04-06

Merzaban JS, Imitola J, Starossom SC, Zhu B, Wang Y, Lee J, Ali AJ, Olah M, Abuelela AF, Khoury SJ, Sackstein R. Glycobiology. 2015 Dec;25(12):1392-409. doi: 10.1093/glycob/cwv046. PMID: 26153105 Abstract Neural stem cell (NSC)-based therapies offer potential for neural repair in central nervous system (CNS) inflammatory and degenerative disorders....

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Progress and obstacles towards generating hematopoietic stem cells from pluripotent stem cells

2017-04-06

Lee J, Dykstra B, Sackstein R, Rossi DJ. Curr Opin Hematol. 2015 Jul;22(4):317-23. doi: 10.1097/MOH.0000000000000147. Review. PMID: 26049752 Abstract PURPOSE OF REVIEW: Human pluripotent stem cells (PSCs) have the potential to provide an inexhaustible source of hematopoietic stem cells (HSCs) that could be used in disease modeling and in clinical...

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Integration of systems glycobiology with bioinformatics toolboxes, glycoinformatics resources, and glycoproteomics data

2017-04-06

Liu G, Neelamegham S. Wiley Interdiscip Rev Syst Biol Med. 2015 Jul-Aug;7(4):163-81. doi: 10.1002/wsbm.1296. Review. PMID: 25871730 Abstract The glycome constitutes the entire complement of free carbohydrates and glycoconjugates expressed on whole cells or tissues. 'Systems Glycobiology' is an emerging discipline that aims to quantitatively describe and analyse the...

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FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets

2017-04-06

Begonja AJ, Pluthero FG, Suphamungmee W, Giannini S, Christensen H, Leung R, Lo RW, Nakamura F, Lehman W, Plomann M, Hoffmeister KM, Kahr WH, Hartwig JH, Falet H. Blood. 2015 Jul 2;126(1):80-8. doi: 10.1182/blood-2014-07-587600. PMID: 25838348 Abstract Bin-Amphiphysin-Rvs (BAR) and Fes-CIP4 homology BAR (F-BAR) proteins generate tubular membrane invaginations...

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HCELL Expression on Murine MSC Licenses Pancreatotropism and Confers Durable Reversal of Autoimmune Diabetes in NOD Mice

2017-04-06

Abdi R, Moore R, Sakai S, Donnelly CB, Mounayar M, Sackstein R. Stem Cells. 2015 May;33(5):1523-31. doi: 10.1002/stem.1948. PMID: 25641589 Abstract Type 1 diabetes (T1D) is an immune-mediated disease resulting in destruction of insulin-producing pancreatic beta cells. Mesenchymal stem cells (MSCs) possess potent immunomodulatory properties, garnering increasing attention as...

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The structures of glycophorin C N-glycans, a putative component of the GPC receptor site for Plasmodium falciparum EBA-140 ligand

2017-04-06

Ashline DJ, Duk M, Lukasiewicz J, Reinhold VN, Lisowska E, Jaskiewicz E. Glycobiology. 2015 May;25(5):570-81. doi: 10.1093/glycob/cwu188. PMID: 25552259 Abstract Glycophorins C and D are highly glycosylated integral sialoglycoproteins of human red blood cell membranes carrying the Gerbich blood group antigens. The O- and N-glycosidic chains of the major...

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The glycosylation-dependent interaction of perlecan core protein with LDL: implications for atherosclerosis

2017-04-06

Xu YX, Ashline D, Liu L, Tassa C, Shaw SY, Ravid K, Layne MD, Reinhold V, Robbins PW. J Lipid Res. 2015 Feb;56(2):266-76. doi: 10.1194/jlr.M053017. PMID: 25528754 Abstract Perlecan is a major heparan sulfate (HS) proteoglycan in the arterial wall. Previous studies have linked it to atherosclerosis. Perlecan contains...

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Tools to MSn Sequence and Document the Structures of Glycan Epitopes

2017-04-06

Zhang H, Ashline DJ, Reinhold VN. Discov Subtleties Sugars (2013). 2014 Dec 22;2013:117-131. PMID: 27110600 Abstract Sequential disassembly (MSn) has been applied to fully characterise and document native samples containing glycan epitopes with their synthetic analogues. Both sample types were prepared by methylation, solvent phase extracted, directly infused and...

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ST3Gal-4 is the primary sialyltransferase regulating the synthesis of E-, P-, and L-selectin ligands on human myeloid leukocytes

2017-04-06

Mondal N, Buffone A Jr, Stolfa G, Antonopoulos A, Lau JT, Haslam SM, Dell A, Neelamegham S. Blood. 2015 Jan 22;125(4):687-96. doi: 10.1182/blood-2014-07-588590. PMID: 25498912 Abstract The precise glycosyltransferase enzymes that mediate selectin-ligand biosynthesis in human leukocytes are unknown. This knowledge is important because selectin-mediated cell tethering and rolling...

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The Ashwell-Morell receptor regulates hepatic thrombopoietin production via JAK2-STAT3 signaling

2017-04-06

Grozovsky R, Begonja AJ, Liu K, Visner G, Hartwig JH, Falet H, Hoffmeister KM. Nat Med. 2015 Jan;21(1):47-54. doi: 10.1038/nm.3770. PMID: 25485912 Abstract The hepatic Ashwell-Morell receptor (AMR) can bind and remove desialylated platelets. Here we demonstrate that platelets become desialylated as they circulate and age in blood. Binding...

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Dynamin 2-dependent endocytosis is required for normal megakaryocyte development in mice

2017-04-06

Bender M, Giannini S, Grozovsky R, Jönsson T, Christensen H, Pluthero FG, Ko A, Mullally A, Kahr WH, Hoffmeister KM, Falet H. Blood. 2015 Feb 5;125(6):1014-24. doi: 10.1182/blood-2014-07-587857. PMID: 25468568 Abstract Dynamins are highly conserved large GTPases (enzymes that hydrolyze guanosine triphosphate) involved in endocytosis and vesicle transport, and...

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Protein and site specificity of fucosylation in liver-secreted glycoproteins

2017-04-06

Pompach P, Ashline DJ, Brnakova Z, Benicky J, Sanda M, Goldman R. J Proteome Res. 2014 Dec 5;13(12):5561-9. doi: 10.1021/pr5005482. PMID: 25265424 Abstract Chronic liver diseases are a serious health problem worldwide. One of the frequently reported glycan alterations in liver disease is aberrant fucosylation, which was suggested as...

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Targeted proteomic assays for the verification of global proteomics insights

2017-04-06

Thomas SN, Zhang H. Expert Rev Proteomics. 2016 Sep 1:1-3. [Epub ahead of print] No abstract available. PMID: 27565203  

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Molecular Functions of Glycoconjugates in Autophagy

2017-04-06

Fahie K, Zachara NE. J Mol Biol. 2016 Aug 14;428(16):3305-24. doi: 10.1016/j.jmb.2016.06.011. Review. PMID: 2734566 Abstract Glycoconjugates, glycans, carbohydrates, and sugars: these terms encompass a class of biomolecules that are diverse in both form and function ranging from free oligosaccharides, glycoproteins, and proteoglycans, to glycolipids that make up a...

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Hyaluronan Depolymerization by Megakaryocyte Hyaluronidase-2 Is Required for Thrombopoiesis

2017-03-23

Petrey AC, Obery DR, Kessler SP, Flamion B, de la Motte CA. Am J Pathol. 2016 Sep;186(9):2390-403. doi: 10.1016/j.ajpath.2016.05.004. PMID: 27398974 Abstract Hyaluronan is the predominant glycosaminoglycan component of the extracellular matrix with an emerging role in hematopoiesis. Modulation of hyaluronan polymer size is responsible for its control over...

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Genetic and biochemical evidence that gastrulation defects in Pofut2 mutants result from defects in ADAMTS9 secretion

2017-03-23

Benz BA, Nandadasa S, Takeuchi M, Grady RC, Takeuchi H, LoPilato RK, Kakuda S, Somerville RP, Apte SS, Haltiwanger RS, Holdener BC. Dev Biol. 2016 Aug 1;416(1):111-22. doi: 10.1016/j.ydbio.2016.05.038. PMID: 27297885 Abstract Protein O-fucosyltransferase 2 (POFUT2) adds O-linked fucose to Thrombospondin Type 1 Repeats (TSR) in 49 potential target...

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Immunoregulatory roles of versican proteolysis in the myeloma microenvironment

2017-03-23

Hope C, Foulcer S, Jagodinsky J, Chen SX, Jensen JL, Patel S, Leith C, Maroulakou I, Callander N, Miyamoto S, Hematti P, Apte SS, Asimakopoulos F. Blood. 2016 Aug 4;128(5):680-5. doi: 10.1182/blood-2016-03-705780. PMID: 27259980 Abstract Myeloma immunosurveillance remains incompletely understood. We have demonstrated proteolytic processing of the matrix proteoglycan,...

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Hyaluronidase and Hyaluronan Oligosaccharides Promote Neurological Recovery after Intraventricular Hemorrhage

2017-03-23

Vinukonda G, Dohare P, Arshad A, Zia MT, Panda S, Korumilli R, Kayton R, Hascall VC, Lauer ME, Ballabh P. J Neurosci. 2016 Jan 20;36(3):872-89. doi: 10.1523/JNEUROSCI.3297-15.2016. PMID: 26791217 Abstract Intraventricular hemorrhage (IVH) in premature infants results in inflammation, arrested oligodendrocyte progenitor cell (OPC) maturation, and reduced myelination of...

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Anti-ADAMTS5 monoclonal antibodies: implications for aggrecanase inhibition in osteoarthritis

2017-03-23

Apte SS. Biochem J. 2016 Jan 1;473(1):e1-4. doi: 10.1042/BJ20151072. Review. PMID: 26657033 Abstract The extracellular matrix of articular cartilage is structurally specialized for efficient absorption of mechanical impact. In particular, giant aggregates of the large chondroitin sulfate proteoglycan, aggrecan, with the glycosaminoglycan, hyaluronan, allow cartilage to resist compressive load....

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Hyaluronan Rafts on Airway Epithelial Cells

2017-03-23

Abbadi A, Lauer M, Swaidani S, Wang A, Hascall V. J Biol Chem. 2016 Jan 15;291(3):1448-55. doi: 10.1074/jbc.M115.704288. PMID: 26601955 Abstract Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This...

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Hyaluronan's Role in Fibrosis: A Pathogenic Factor or a Passive Player?

2017-03-23

Albeiroti S, Soroosh A, de la Motte CA. Biomed Res Int. 2015;2015:790203. doi: 10.1155/2015/790203. Review. PMID: 26583132 Abstract Fibrosis is a debilitating condition that can lead to impairment of the affected organ's function. Excessive deposition of extracellular matrix (ECM) molecules is characteristic of most fibrotic tissues. Fibroblasts activated by...

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Hyaluronan Synthase 3 Null Mice Exhibit Decreased Intestinal Inflammation and Tissue Damage in the DSS-Induced Colitis Model

2017-03-23

Kessler SP, Obery DR, de la Motte C. Int J Cell Biol. 2015;2015:745237. doi: 10.1155/2015/745237. PMID: 26448758 Abstract Hyaluronan (HA) overproduction is a hallmark of multiple inflammatory diseases, including inflammatory bowel disease (IBD). Hyaluronan can act as a leukocyte recruitment molecule and in the most common mouse model of...

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The Rise and Fall of Hyaluronan in Respiratory Diseases

2017-03-23

Lauer ME, Dweik RA, Garantziotis S, Aronica MA. Int J Cell Biol. 2015;2015:712507. doi: 10.1155/2015/712507. Review. PMID: 26448757 Abstract In normal airways, hyaluronan (HA) matrices are primarily located within the airway submucosa, pulmonary vasculature walls, and, to a lesser extent, the alveoli. Following pulmonary injury, elevated levels of HA...

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Hyperglycemia-Induced Changes in Hyaluronan Contribute to Impaired Skin Wound Healing in Diabetes: Review and Perspective

2017-03-23

Shakya S, Wang Y, Mack JA, Maytin EV. Int J Cell Biol. 2015;2015:701738. doi: 10.1155/2015/701738. Review. PMID: 26448756 Abstract Ulcers and chronic wounds are a particularly common problem in diabetics and are associated with hyperglycemia. In this targeted review, we summarize evidence suggesting that defective wound healing in diabetics...

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Utilization of Glycosaminoglycans/Proteoglycans as Carriers for Targeted Therapy Delivery

2017-03-23

Misra S, Hascall VC, Atanelishvili I, Moreno Rodriguez R, Markwald RR, Ghatak S. Int J Cell Biol. 2015;2015:537560. doi: 10.1155/2015/537560. Review. PMID: 26448753 Abstract The outcome of patients with cancer has improved significantly in the past decade with the incorporation of drugs targeting cell surface adhesive receptors, receptor tyrosine...

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ADAMTS3 activity is mandatory for embryonic lymphangiogenesis and regulates placental angiogenesis

2017-03-23

Janssen L, Dupont L, Bekhouche M, Noel A, Leduc C, Voz M, Peers B, Cataldo D, Apte SS, Dubail J, Colige A. Angiogenesis. 2016 Jan;19(1):53-65. doi: 10.1007/s10456-015-9488-z. PMID: 26446156 Abstract The only documented activity of a subclass of ADAMTS proteases comprising ADAMTS2, 3 and 14 is the cleavage of...

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The Responses of Hyperglycemic Dividing Mesangial Cells to Heparin Are Mediated by the Non-reducing Terminal Trisaccharide

2017-03-23

Wang CP, Hascall VC, Zhang F, Linhardt RJ, Abbadi A, Wang A. J Biol Chem. 2015 Nov 27;290(48):29045-50. doi: 10.1074/jbc.M115.677401. PMID: 26378235 Abstract Our previous studies showed: (i) that growth-arrested G0/G1 rat mesangial cells stimulated to divide in hyperglycemic medium initiate intracellular hyaluronan synthesis that induces autophagy and the...

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Hyaluronan and Its Heavy Chain Modification in Asthma Severity and Experimental Asthma Exacerbation

2017-03-23

Lauer ME, Majors AK, Comhair S, Ruple LM, Matuska B, Subramanian A, Farver C, Dworski R, Grandon D, Laskowski D, Dweik RA, Erzurum SC, Hascall VC, Aronica MA. J Biol Chem. 2015 Sep 18;290(38):23124-34. doi: 10.1074/jbc.M115.663823. PMID: 26209637 Abstract Hyaluronan (HA) is a large (>1500 kDa) polysaccharide of the...

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Elevated pulmonary pressure: A novel risk marker in kidney disease?

2017-03-23

Navaneethan SD, Dweik RA. Kidney Int. 2015 Jul;88(1):7-9. doi: 10.1038/ki.2015.111. PMID: 26126087 Abstract Pulmonary hypertension is associated with adverse cardiac outcomes and death in the general population. Bolignano and colleagues studied the prevalence of higher estimated pulmonary artery systolic pressure (⩾35 mm Hg) assessed using echocardiogram among patients with stage 1-4...

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ADAMTS9-Mediated Extracellular Matrix Dynamics Regulates Umbilical Cord Vascular Smooth Muscle Differentiation and Rotation

2017-03-23

Nandadasa S, Nelson CM, Apte SS. Cell Rep. 2015 Jun 16;11(10):1519-28. doi: 10.1016/j.celrep.2015.05.005. PMID: 26027930 Abstract Despite the significance for fetal nourishment in mammals, mechanisms of umbilical cord vascular growth remain poorly understood. Here, the secreted metalloprotease ADAMTS9 is shown to be necessary for murine umbilical cord vascular development....

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Outcomes of β-blocker use in pulmonary arterial hypertension: a propensity-matched analysis

2017-03-23

Bandyopadhyay D, Bajaj NS, Zein J, Minai OA, Dweik RA. Eur Respir J. 2015 Sep;46(3):750-60. doi: 10.1183/09031936.00215514. PMID: 26022959 Abstract The utility and safety of β-blockers in pulmonary hypertension is controversial. Anecdotal reports suggest that β-blockers may be harmful in these patients. The aim of our study was to...

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Interactions between Hyaluronan and Its Receptors (CD44, RHAMM) Regulate the Activities of Inflammation and Cancer

2017-03-23

Misra S, Hascall VC, Markwald RR, Ghatak S. Front Immunol. 2015 May 6;6:201. doi: 10.3389/fimmu.2015.00201. Review. PMID: 25999946 Abstract The glycosaminoglycan hyaluronan (HA), a major component of extracellular matrices, and cell surface receptors of HA have been proposed to have pivotal roles in cell proliferation, migration, and invasion, which...

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The role of hyaluronan in innate defense responses of the intestine

2017-03-23

de la Motte CA, Kessler SP. Int J Cell Biol. 2015;2015:481301. doi: 10.1155/2015/481301. Review. PMID: 25922605 Abstract Hyaluronan is an abundant extracellular matrix component prevalent in the vertebrate intestinal tract. Here we discuss what is known about hyaluronan distribution during homeostasis and inflammatory diseases of the gut and discuss...

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Analysis of breath volatile organic compounds in children with chronic liver disease compared to healthy controls

2017-03-23

Eng K, Alkhouri N, Cikach F, Patel N, Yan C, Grove D, Lopez R, Rome E, Dweik RA. J Breath Res. 2015 Apr 20;9(2):026002. doi: 10.1088/1752-7155/9/2/026002. PMID: 25891513 Abstract Breath testing is increasingly being used as a non-invasive diagnostic tool for disease states across medicine. The purpose of this...

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The epithelial danger signal IL-1α is a potent activator of fibroblasts and reactivator of intestinal inflammation

2017-03-23

Scarpa M, Kessler S, Sadler T, West G, Homer C, McDonald C, de la Motte C, Fiocchi C, Stylianou E. Am J Pathol. 2015 Jun;185(6):1624-37. doi: 10.1016/j.ajpath.2015.02.018. PMID: 25864926 Abstract Intestinal epithelial cell (IEC) death is typical of inflammatory bowel disease (IBD). We investigated: i) whether IEC-released necrotic cell...

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Deletion of ADAMTS5 does not affect aggrecan or versican degradation but promotes glucose uptake and proteoglycan synthesis in murine adipose derived stromal cells

2017-03-23

Gorski DJ, Xiao W, Li J, Luo W, Lauer M, Kisiday J, Plaas A, Sandy J. Matrix Biol. 2015 Sep;47:66-84. doi: 10.1016/j.matbio.2015.03.008. PMID: 25840345 Abstract ADAMTS5 (TS5), a member of the aggrecanase clade (TS1, 4, 5, 8, 9, 15) of ADAMTS-proteases, has been considered largely responsible for the proteolysis...

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Insights on ADAMTS proteases and ADAMTS-like proteins from mammalian genetics

2017-03-23

Dubail J, Apte SS. Matrix Biol. 2015 May-Jul;44-46:24-37. doi: 10.1016/j.matbio.2015.03.001. Review. PMID: 25770910 Abstract The mammalian ADAMTS superfamily comprises 19 secreted metalloproteinases and 7 ADAMTS-like proteins, each the product of a distinct gene. Thus far, all appear to be relevant to extracellular matrix function or to cell-matrix interactions. Most...

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Shot-noise limited Faraday rotation spectroscopy for detection of nitric oxide isotopes in breath, urine, and blood

2017-03-23

Wang Y, Nikodem M, Zhang E, Cikach F, Barnes J, Comhair S, Dweik RA, Kao C, Wysocki G. Sci Rep. 2015 Mar 13;5:9096. doi: 10.1038/srep09096. PMID: 25767064 Abstract Measurement of NO and/or its metabolites in the various body compartments has transformed our understanding of biology. The inability of the...

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Volatile Organic Compounds in Urine for Noninvasive Diagnosis of Malignant Biliary Strictures: A Pilot Study

2017-03-23

Navaneethan U, Parsi MA, Lourdusamy D, Grove D, Sanaka MR, Hammel JP, Vargo JJ, Dweik RA. Dig Dis Sci. 2015 Jul;60(7):2150-7. doi: 10.1007/s10620-015-3596-x. PMID: 25708900 Abstract BACKGROUND: The use of volatile organic compounds (VOCs) in bile was recently studied and appeared promising for diagnosis of malignancy. Noninvasive diagnosis of malignant...

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Chromosome-associated protein D3 promotes bacterial clearance in human intestinal epithelial cells by repressing expression of amino acid transporters

2017-03-23

Schuster AT, Homer CR, Kemp JR, Nickerson KP, Deutschman E, Kim Y, West G, Sadler T, Stylianou E, Krokowski D, Hatzoglou M, de la Motte C, Rubin BP, Fiocchi C, McDonald C, Longworth MS. Gastroenterology. 2015 Jun;148(7):1405-16.e3. doi: 10.1053/j.gastro.2015.02.013. PMID: 25701737 Abstract BACKGROUND & AIMS: Defects in colonic epithelial barrier...

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Hyperglycemia diverts dividing stem cells to pathological adipogenesis

2017-03-23

Hascall V, Wang A. Stem Cell Res Ther. 2014 Nov 17;5(6):128. doi: 10.1186/scrt518. PMID: 25689074 Abstract This commentary proposes a mechanism for why murine diabetic adipose tissue contains very few remaining stem cells compared with normal adipose tissue. The mechanism involves the diversion of stem cells to pathological adipocytes...

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O-linked β-N-acetylglucosamine transferase directs cell proliferation in idiopathic pulmonary arterial hypertension

2017-03-23

Barnes JW, Tian L, Heresi GA, Farver CF, Asosingh K, Comhair SA, Aulak KS, Dweik RA. Circulation. 2015 Apr 7;131(14):1260-8. doi: 10.1161/CIRCULATIONAHA.114.013878. PMID: 25663381 Abstract BACKGROUND: Idiopathic pulmonary arterial hypertension (IPAH) is a cardiopulmonary disease characterized by cellular proliferation and vascular remodeling. A more recently recognized characteristic of the disease...

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Are transcutaneous oxygen and carbon dioxide determinations of value in pulmonary arterial hypertension?

2017-03-23

Tonelli AR, Alkukhun L, Cikach F, Ahmed M, Dweik RA. Microcirculation. 2015 May;22(4):249-56. doi: 10.1111/micc.12191. PMID: 25641509 Abstract BACKGROUND: We hypothesized that transcutaneous gas determinations of O2 and CO2 (TcPO2 and TcPCO2 ) are associated with the severity of PAH. METHODS: In this cross-sectional study, we included consecutive patients with PAH (group...

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Determination of hyaluronan molecular mass distribution in human breast milk

2017-03-23

Yuan H, Amin R, Ye X, de la Motte CA, Cowman MK. Anal Biochem. 2015 Apr 1;474:78-88. doi: 10.1016/j.ab.2014.12.020. PMID: 25579786 Abstract Hyaluronan (HA) in human milk mediates host responses to microbial infection via TLR4- and CD44-dependent signaling. Signaling by HA is generally size specific. Because pure HA with...

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Review article: breath analysis in inflammatory bowel diseases

2017-03-23

Kurada S, Alkhouri N, Fiocchi C, Dweik R, Rieder F. Aliment Pharmacol Ther. 2015 Feb;41(4):329-41. doi: 10.1111/apt.13050. Review. PMID: 25523187 Abstract BACKGROUND: There is an urgent need for cheap, reproducible, easy to perform and specific biomarkers for diagnosis, differentiation and stratification of inflammatory bowel disease (IBD) patients. Technical advances allow...

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Volatile organic compounds in bile for early diagnosis of cholangiocarcinoma in patients with primary sclerosing cholangitis: a pilot study

2017-03-23

Navaneethan U, Parsi MA, Lourdusamy V, Bhatt A, Gutierrez NG, Grove D, Sanaka MR, Hammel JP, Stevens T, Vargo JJ, Dweik RA. Gastrointest Endosc. 2015 Apr;81(4):943-9.e1. doi: 10.1016/j.gie.2014.09.041. PMID: 25500329 Abstract BACKGROUND: The diagnosis of cholangiocarcinoma (CCA) in patients with primary sclerosing cholangitis (PSC) is particularly difficult. The role of...

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Platelet hyaluronidase-2: an enzyme that translocates to the surface upon activation to function in extracellular matrix degradation

2017-03-23

Albeiroti S, Ayasoufi K, Hill DR, Shen B, de la Motte CA. Blood. 2015 Feb 26;125(9):1460-9. doi: 10.1182/blood-2014-07-590513. PMID: 25411425 Abstract Following injury, platelets rapidly interact with the exposed extracellular matrix (ECM) of the vessel wall and the surrounding tissues. Hyaluronan (HA) is a major glycosaminoglycan component of the...

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Analysis of the heavy-chain modification and TSG-6 activity in pathological hyaluronan matrices

2017-03-23

Lauer ME, Loftis J, de la Motte C, Hascall VC. Methods Mol Biol. 2015;1229:543-8. doi: 10.1007/978-1-4939-1714-3_42. PMID: 25325979 Abstract During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred to hyaluronan (HA) via the enzyme tumor-necrosis-factor-stimulated-gene 6 (TSG-6) to form a HC-HA complex. In this...

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A new Adamts9 conditional mouse allele identifies its non-redundant role in interdigital web regression

2017-03-23

Dubail J, Aramaki-Hattori N, Bader HL, Nelson CM, Katebi N, Matuska B, Olsen BR, Apte SS. Genesis. 2014 Jul;52(7):702-12. doi: 10.1002/dvg.22784. PMID: 24753090 Abstract ADAMTS9 is the most conserved member of a large family of secreted metalloproteases having diverse functions. Adamts9 null mice die before gastrulation, precluding investigations of...

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Breathprints of childhood obesity: changes in volatile organic compounds in obese children compared with lean controls

2017-03-23

Alkhouri N, Eng K, Cikach F, Patel N, Yan C, Brindle A, Rome E, Hanouneh I, Grove D, Lopez R, Hazen SL, Dweik RA. Pediatr Obes. 2015 Feb;10(1):23-9. doi: 10.1111/j.2047-6310.2014.221.x. PMID: 24677760 Abstract OBJECTIVE: The objective of this study was to investigate changes in volatile organic compounds (VOCs) in exhaled...

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Subcutaneous to intravenous prostacyclin analog transition in pulmonary hypertension

2017-03-23

Alkukhun L, Bair ND, Dweik RA, Tonelli AR. J Cardiovasc Pharmacol. 2014 Jan;63(1):4-8. doi: 10.1097/FJC.0000000000000018. PMID: 24084219 Abstract INTRODUCTION: Prostacyclin analogs are Food and Drug Administration-approved therapies for the treatment of pulmonary arterial hypertension and can be administered by inhalational, intravenous (IV), or subcutaneous (SQ) routes. Because there are limited...

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Broad Spectrum Anti-Influenza Agents by Inhibiting Self-Association of Matrix Protein 1

2017-03-23

Mosier PD, Chiang MJ, Lin Z, Gao Y, Althufairi B, Zhou Q, Musayev F, Safo MK, Xie H, Desai UR. Sci Rep. 2016 Aug 30;6:32340. doi: 10.1038/srep32340. PMID: 27573445 Abstract The matrix protein 1 (M1) of influenza A virus (IAV) exists as a three-dimensional oligomeric structure in mature virions...

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Thyroid hormone: relevance to xenotransplantation

2017-03-23

Iwase H, Ekser B, Hara H, Ezzelarab MB, Long C, Thomson AW, Ayares D, Cooper DK. Xenotransplantation. 2016 Jul;23(4):293-9. doi: 10.1111/xen.12243. PMID: 27374212 Abstract BACKGROUND: It has been well documented that the level of serum/plasma free triiodothyronine (fT3) falls rapidly following brain death or during certain surgical procedures, for example,...

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Luminal Extracellular Vesicles (EVs) in Inflammatory Bowel Disease (IBD) Exhibit Proinflammatory Effects on Epithelial Cells and Macrophages

2017-03-23

Mitsuhashi S, Feldbrügge L, Csizmadia E, Mitsuhashi M, Robson SC, Moss AC. Inflamm Bowel Dis. 2016 Jul;22(7):1587-95. doi: 10.1097/MIB.0000000000000840. PMID: 27271497 Abstract BACKGROUND: Extracellular vesicles (EVs) are membrane-enclosed particles released by cells as a means of intercellular communication. They are potential novel biomarkers, as they are readily isolated from body...

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Allosteric Partial Inhibition of Monomeric Proteases. Sulfated Coumarins Induce Regulation, not just Inhibition, of Thrombin

2017-03-23

Verespy S 3rd, Mehta AY, Afosah D, Al-Horani RA, Desai UR. Sci Rep. 2016 Apr 7;6:24043. doi: 10.1038/srep24043. PMID: 27053426 Abstract Allosteric partial inhibition of soluble, monomeric proteases can offer major regulatory advantages, but remains a concept on paper to date; although it has been routinely documented for receptors...

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Factor XIa inhibitors: A review of the patent literature

2017-03-23

Al-Horani RA, Desai UR. Expert Opin Ther Pat. 2016;26(3):323-45. doi: 10.1517/13543776.2016.1154045. Review. PMID: 26881476 Abstract INTRODUCTION: Anticoagulants are the mainstay for prevention and/or treatment of thrombotic disorders. Each clinically used anticoagulant is associated with significant adverse consequences, especially bleeding. Factor XIa (FXIa), a key factor involved in the amplification of...

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Allosterism-based simultaneous, dual anticoagulant and antiplatelet action: allosteric inhibitor targeting the glycoprotein Ibα-binding and heparin-binding site of thrombin

2017-03-23

Mehta AY, Mohammed BM, Martin EJ, Brophy DF, Gailani D, Desai UR. J Thromb Haemost. 2016 Apr;14(4):828-38. doi: 10.1111/jth.13254. PMID: 26748875 Abstract BACKGROUND: Allosteric inhibition is a promising approach for developing a new group of anticoagulants with potentially reduced bleeding consequences. Recently, we designed sulfated β-O4 lignin (SbO4L) as an...

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CXCL1/MGSA Is a Novel Glycosaminoglycan (GAG)-binding Chemokine: STRUCTURAL EVIDENCE FOR TWO DISTINCT NON-OVERLAPPING BINDING DOMAINS

2017-03-23

Sepuru KM, Rajarathnam K. J Biol Chem. 2016 Feb 19;291(8):4247-55. doi: 10.1074/jbc.M115.697888. PMID: 26721883 Abstract In humans, the chemokine CXCL1/MGSA (hCXCL1) plays fundamental and diverse roles in pathophysiology, from microbial killing to cancer progression, by orchestrating the directed migration of immune and non-immune cells. Cellular trafficking is highly regulated...

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Recent advances in understanding xenotransplantation: implications for the clinic

2017-03-23

Cooper DK, Bottino R. Expert Rev Clin Immunol. 2015;11(12):1379-90. doi: 10.1586/1744666X.2015.1083861. PMID: 26548357 Abstract The results of organ and cell allotransplantation continue to improve, but the field remains limited by a lack of deceased donor organs. Xenotransplantation, for example, between pig and human, offers unlimited organs and cells for...

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A Simple Method for Discovering Druggable, Specific Glycosaminoglycan-Protein Systems. Elucidation of Key Principles from Heparin/Heparan Sulfate-Binding Proteins

2017-03-23

Sarkar A, Desai UR. PLoS One. 2015 Oct 21;10(10):e0141127. doi: 10.1371/journal.pone.0141127. PMID: 26488293 Abstract Glycosaminoglycans (GAGs) affect human physiology and pathology by modulating more than 500 proteins. GAG-protein interactions are generally assumed to be ionic and nonspecific, but specific interactions do exist. Here, we present a simple method to...

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In vitro testing of an anti-CD40 monoclonal antibody, clone 2C10, in primates and pigs

2017-03-23

Lee W, Satyananda V, Iwase H, Tanaka T, Miyagawa Y, Long C, Ayares D, Cooper DK, Hara H. Transpl Immunol. 2015 Nov;33(3):185-91. doi: 10.1016/j.trim.2015.09.007. PMID: 26458513 Abstract BACKGROUND: The CD40/CD154 and CD28/B7 pathways are important in allo- and xeno-transplantation. Owing to the thrombotic complications of anti-CD154mAb, anti-CD40mAb has emerged as...

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Solution NMR characterization of chemokine CXCL8/IL-8 monomer and dimer binding to glycosaminoglycans: structural plasticity mediates differential binding interactions

2017-03-23

Joseph PR, Mosier PD, Desai UR, Rajarathnam K. Biochem J. 2015 Nov 15;472(1):121-33. doi: 10.1042/BJ20150059. PMID: 26371375 Abstract Chemokine CXCL8/interleukin-8 (IL-8) plays a crucial role in directing neutrophils and oligodendrocytes to combat infection/injury and tumour cells in metastasis development. CXCL8 exists as monomers and dimers and interaction of both...

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Further evidence for sustained systemic inflammation in xenograft recipients (SIXR)

2017-03-23

Iwase H, Ekser B, Zhou H, Liu H, Satyananda V, Humar R, Humar P, Hara H, Long C, Bhama JK, Bajona P, Wang Y, Wijkstrom M, Ayares D, Ezzelarab MB, Cooper DK. Xenotransplantation. 2015 Sep-Oct;22(5):399-405. doi: 10.1111/xen.12182. PMID: 26292982 Abstract INTRODUCTION: In pig-to-baboon heart/artery patch transplantation models, adequate costimulation blockade...

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Flexible Linker Modulates Glycosaminoglycan Affinity of Decorin Binding Protein A

2017-03-23

Morgan A, Sepuru KM, Feng W, Rajarathnam K, Wang X. Biochemistry. 2015 Aug 18;54(32):5113-9. doi: 10.1021/acs.biochem.5b00253. PMID: 26223367 Abstract Decorin binding protein A (DBPA) is a glycosaminoglycan (GAG)-binding adhesin found on the surface of the bacterium Borrelia burgdorferi (B. burgdorferi), the causative agent of Lyme disease. DBPA facilitates bacterial...

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Chemokine CXCL1-Mediated Neutrophil Trafficking in the Lung: Role of CXCR2 Activation

2017-03-23

Sawant KV, Xu R, Cox R, Hawkins H, Sbrana E, Kolli D, Garofalo RP, Rajarathnam K. J Innate Immun. 2015;7(6):647-58. doi: 10.1159/000430914. PMID: 26138727 Abstract The chemokine CXCL1 and its receptor CXCR2 play a crucial role in host immune response by recruiting and activating neutrophils for microbial killing at...

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Experimental hepatocyte xenotransplantation--a comprehensive review of the literature

2017-03-23

Zhou H, Liu H, Ezzelarab M, Schmelzer E, Wang Y, Gerlach J, Gridelli B, Cooper DK. Xenotransplantation. 2015 Jul-Aug;22(4):239-48. doi: 10.1111/xen.12170. Review. PMID: 25950141 Abstract Hepatocyte transplantation (Tx) is a potential therapy for certain diseases of the liver, including hepatic failure. However, there is a limited supply of human...

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Four-dimensional characterization of thrombosis in a live-cell, shear-flow assay: development and application to xenotransplantation

2017-03-23

Harris DG, Benipal PK, Cheng X, Burdorf L, Azimzadeh AM, Pierson RN 3rd. PLoS One. 2015 Apr 1;10(4):e0123015. doi: 10.1371/journal.pone.0123015. PMID: 25830912 Abstract BACKGROUND: Porcine xenografts are a promising source of scarce transplantable organs, but stimulate intense thrombosis of human blood despite targeted genetic and pharmacologic interventions. Current experimental models...

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The case for xenotransplantation

2017-03-23

Cooper DK. Clin Transplant. 2015 Apr;29(4):288-93. doi: 10.1111/ctr.12522. PMID: 25728841 Abstract The availability of organs and cells from deceased humans for transplantation is not meeting the demand. Xenotransplantation, specifically the transplantation of organs and cells from genetically engineered pigs, could resolve this problem. Diabetic monkeys have remained normoglycemic and...

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Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs

2017-03-23

Iwase H, Ekser B, Satyananda V, Zhou H, Hara H, Bajona P, Wijkstrom M, Bhama JK, Long C, Veroux M, Wang Y, Dai Y, Phelps C, Ayares D, Ezzelarab MB, Cooper DK. Transpl Immunol. 2015 Mar;32(2):99-108. doi: 10.1016/j.trim.2015.02.003. PMID: 25687023 Abstract BACKGROUND: In the pig-to-nonimmunosuppressed baboon artery patch model, a...

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Investigation of the heparin-thrombin interaction by dynamic force spectroscopy

2017-03-23

Wang C, Jin Y, Desai UR, Yadavalli VK. Biochim Biophys Acta. 2015 Jun;1850(6):1099-106. doi: 10.1016/j.bbagen.2015.01.016. PMID: 25647100 Abstract BACKGROUND: The interaction between heparin and thrombin is a vital step in the blood (anti)coagulation process. Unraveling the molecular basis of the interactions is therefore extremely important in understanding the mechanisms of...

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Solution NMR characterization of WT CXCL8 monomer and dimer binding to CXCR1 N-terminal domain

2017-03-23

Joseph PR, Rajarathnam K. Protein Sci. 2015 Jan;24(1):81-92. doi: 10.1002/pro.2590. PMID: 25327289 Abstract Chemokine CXCL8 and its receptor CXCR1 are key mediators in combating infection and have also been implicated in the pathophysiology of various diseases including chronic obstructive pulmonary disease (COPD) and cancer. CXCL8 exists as monomers and...

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Synthesis and biomedical applications of xylosides

2017-03-23

Kalita M, Quintero MV, Raman K, Tran VM, Kuberan B. Methods Mol Biol. 2015;1229:517-28. doi: 10.1007/978-1-4939-1714-3_40. PMID: 25325977 Abstract Xylosides modulate the biosynthesis of sulfated glycosaminoglycans (GAGs) in various cell types. A new class of xylosides called "click-xylosides" has been synthesized for their biostability, ease of chemical synthesis, and...

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Murine models in the evaluation of heparan sulfate-based anticoagulants

2017-03-23

Gailani D, Cheng Q, Ivanov IS. Methods Mol Biol. 2015;1229:483-96. doi: 10.1007/978-1-4939-1714-3_37. PMID: 25325974 Abstract Evaluating anticoagulants in animal thrombosis models is a standard component of preclinical drug testing. Mice are frequently used for these initial evaluations because a variety of thrombosis models have been developed and are well...

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Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy

2017-03-23

Joseph PR, Poluri KM, Sepuru KM, Rajarathnam K. Methods Mol Biol. 2015;1229:325-33. doi: 10.1007/978-1-4939-1714-3_26. PMID: 25325963 Abstract Solution nuclear magnetic resonance (NMR) spectroscopy and, in particular, chemical shift perturbation (CSP) titration experiments are ideally suited for characterizing the binding interface of macromolecular complexes. (1)H-(15) N-HSQC-based CSP studies have become the...

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Using isothermal titration calorimetry to determine thermodynamic parameters of protein-glycosaminoglycan interactions

2017-03-23

Dutta AK, Rösgen J, Rajarathnam K. Methods Mol Biol. 2015;1229:315-24. doi: 10.1007/978-1-4939-1714-3_25. Erratum in: Methods Mol Biol. 2015;1229:E1. PMID: 25325962 Abstract It has now become increasingly clear that a complete atomic description of how biomacromolecules recognize each other requires knowledge not only of the structures of the complexes but...

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A rapid, nonradioactive assay for measuring heparan sulfate C-5 epimerase activity using hydrogen/deuterium exchange-mass spectrometry

2017-03-23

Babu P, Victor XV, Raman K, Kuberan B. Methods Mol Biol. 2015;1229:209-19. doi: 10.1007/978-1-4939-1714-3_19. PMID: 25325956 Abstract Heparin and heparan sulfate (HS) glycosaminoglycans have important roles in anticoagulation, human development, and human diseases. HS C5-epimerase, which catalyzes the epimerization of GlcA to IdoA, is a crucial enzyme involved in...

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Synthesis of selective inhibitors of heparan sulfate and chondroitin sulfate proteoglycan biosynthesis

2017-03-23

Mencio C, Garud DR, Kuberan B, Koketsu M. Methods Mol Biol. 2015;1229:69-78. doi: 10.1007/978-1-4939-1714-3_8. PMID: 25325945 Abstract Glycosaminoglycan (GAG) side chains of proteoglycans are involved in a wide variety of developmental and pathophysiological functions. Similar to a gene knockout, the ability to inhibit GAG biosynthesis would allow us to...

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Preparation of isotope-enriched heparan sulfate precursors for structural biology studies

2017-03-23

Victor XV, Tran VM, Kuberan B, Nguyen TK. Methods Mol Biol. 2015;1229:43-8. doi: 10.1007/978-1-4939-1714-3_6. PMID: 25325943 Abstract Heparan sulfate (HS) plays numerous important roles in biological systems through their interactions with a wide array of proteins. Structural biology studies of heparan sulfate are often challenging due to the heterogeneity...

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Synthesis of sulfur isotope-labeled sulfate donor, 3'-phosphoadenosine-5'-phosphosulfate, for studying glycosaminoglycan functions

2017-03-23

Mencio C, Swarup VP, Soliai M, Kuberan B. Methods Mol Biol. 2015;1229:37-42. doi: 10.1007/978-1-4939-1714-3_5. PMID: 25325942 Abstract The biological activity of glycosaminoglycans (GAGs) depends greatly on the sulfation pattern present within the GAG chain. Chemical biology of GAGs can be further advanced by preparation of sulfur-isotope-enriched sulfated GAGs. 3'-Phosphoadenosine-5'-phosphosulfate...

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Chemical modification of heparin and heparosan

2017-03-23

Raman K, Kuberan B, Arungundram S. Methods Mol Biol. 2015;1229:31-6. doi: 10.1007/978-1-4939-1714-3_4. PMID: 25325941 Abstract Heparin is a potent clinically used anticoagulant. It is a heterogeneous mixture of polymers that contain a variety of sulfation patterns. However, only 3-O sulfonated heparin pentasaccharide units have been proven to bind to...

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Production of size-defined heparosan, heparan sulfate, and heparin oligosaccharides by enzymatic depolymerization

2017-03-23

Brown S, Kuberan B. Methods Mol Biol. 2015;1229:21-9. doi: 10.1007/978-1-4939-1714-3_3. PMID: 25325940 Abstract Glycosaminoglycans (GAG) are most commonly isolated as large polymers from various animal origins, the functional units of which are oligosaccharides, which bind their target proteins to induce conformational changes, compete with other ligands, or facilitate the...

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Enzymatic synthesis of heparan sulfate and heparin

2017-03-23

Joice A, Raman K, Mencio C, Quintero MV, Brown S, Nguyen TK, Kuberan B. Methods Mol Biol. 2015;1229:11-9. doi: 10.1007/978-1-4939-1714-3_2. PMID: 25325939 Abstract Heparan sulfate (HS) polysaccharide chains have been shown to orchestrate distinct biological functions in several systems. Study of HS structure-function relations is, however, hampered due to...

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Substantial non-electrostatic forces are needed to induce allosteric disruption of thrombin's active site through exosite 2

2017-03-23

Mehta AY, Desai UR. Biochem Biophys Res Commun. 2014 Sep 26;452(3):813-6. doi: 10.1016/j.bbrc.2014.09.003. PMID: 25201728 Abstract Sulfated β-O4 lignin (SbO4L), a non-saccharide glycosaminoglycan mimetic, was recently disclosed as a novel exosite 2-directed thrombin inhibitor with the capability of mimicking sulfated tyrosine sequences of glycoprotein Ibα resulting in dual anticoagulant...

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A comparison of three methods of decellularization of pig corneas to reduce immunogenicity

2017-03-23

Lee W, Miyagawa Y, Long C, Cooper DK, Hara H. Int J Ophthalmol. 2014 Aug 18;7(4):587-93. doi: 10.3980/j.issn.2222-3959.2014.04.01. PMID: 25161926 Abstract AIM: To investigate whether decellularization using different techniques can reduce immunogenicity of the cornea, and to explore the decellularized cornea as a scaffold for cultured corneal endothelial cells (CECs)....

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Histopathologic insights into the mechanism of anti-non-Gal antibody-mediated pig cardiac xenograft rejection

2017-03-23

Byrne GW, Azimzadeh AM, Ezzelarab M, Tazelaar HD, Ekser B, Pierson RN, Robson SC, Cooper DK, McGregor CG. Xenotransplantation. 2013 Sep-Oct;20(5):292-307. doi: 10.1111/xen.12050. Review. PMID: 25098626 Abstract The histopathology of cardiac xenograft rejection has evolved over the last 20 yr with the development of new modalities for limiting antibody-mediated injury,...

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Identification of prognostic biomarkers in hepatitis B virus-related hepatocellular carcinoma and stratification by integrative multi-omics analysis

2017-03-23

Miao R, Luo H, Zhou H, Li G, Bu D, Yang X, Zhao X, Zhang H, Liu S, Zhong Y, Zou Z, Zhao Y, Yu K, He L, Sang X, Zhong S, Huang J, Wu Y, Miksad RA, Robson SC, Jiang C, Zhao Y, Zhao...

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Deficiency of Plasminogen Receptor, Plg-RKT, Causes Defects in Plasminogen Binding and Inflammatory Macrophage Recruitment in vivo

2017-03-23

Miles LA, Baik N, Lighvani S, Khaldoyanidi S, Varki NM, Bai H, Mueller BM, Parmer RJ. J Thromb Haemost. 2016 Oct 7. doi: 10.1111/jth.13532. [Epub ahead of print] PMID: 27714956 Abstract Essentials Plg-RKT is a novel integral membrane plasminogen receptor. The functions of Plg-RKT in vivo are not known. Plg-RKT is a...

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Biological Roles of Glycans

2017-03-23

Varki A. Glycobiology. 2016 Aug 24. pii: cww086. [Epub ahead of print] Review. PMID: 27558841 Abstract Simple and complex carbohydrates (glycans) have long been known to play major metabolic, structural and physical roles in biological systems. Targeted microbial binding to host glycans has also been studied for decades. But...

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Antimicrobial Peptide LL37 and MAVS Signaling Drive Interferon-β Production by Epidermal Keratinocytes during Skin Injury

2017-03-23

Zhang LJ, Sen GL, Ward NL, Johnston A, Chun K, Chen Y, Adase C, Sanford JA, Gao N, Chensee M, Sato E, Fritz Y, Baliwag J, Williams MR, Hata T, Gallo RL. Immunity. 2016 Jul 19;45(1):119-30. doi: 10.1016/j.immuni.2016.06.021. PMID: 27438769 Abstract Type 1 interferons (IFNs) promote inflammation in the...

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ApoC-III inhibits clearance of triglyceride-rich lipoproteins through LDL family receptors

2017-03-23

Gordts PL, Nock R, Son NH, Ramms B, Lew I, Gonzales JC, Thacker BE, Basu D, Lee RG, Mullick AE, Graham MJ, Goldberg IJ, Crooke RM, Witztum JL, Esko JD. J Clin Invest. 2016 Aug 1;126(8):2855-66. doi: 10.1172/JCI86610. PMID: 27400128 Abstract Hypertriglyceridemia is an independent risk factor for cardiovascular...

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Evolutionary inactivation of a sialidase in group B Streptococcus

2017-03-23

Yamaguchi M, Hirose Y, Nakata M, Uchiyama S, Yamaguchi Y, Goto K, Sumitomo T, Lewis AL, Kawabata S, Nizet V. Sci Rep. 2016 Jun 29;6:28852. doi: 10.1038/srep28852. PMID: 27352769 Abstract Group B Streptococcus (GBS) is a leading cause of bacterial sepsis and meningitis in newborns. GBS possesses a protein...

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Expanding the 3-O-Sulfate Proteome--Enhanced Binding of Neuropilin-1 to 3-O-Sulfated Heparan Sulfate Modulates Its Activity

2017-03-23

Thacker BE, Seamen E, Lawrence R, Parker MW, Xu Y, Liu J, Vander Kooi CW, Esko JD. ACS Chem Biol. 2016 Apr 15;11(4):971-80. doi: 10.1021/acschembio.5b00897. PMID: 26731579 Abstract Binding of proteins to heparan sulfate is driven predominantly by electrostatic interactions between positively charged amino acid residues in the protein...

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Utilizing CMP-Sialic Acid Analogs to Unravel Neisseria gonorrhoeae Lipooligosaccharide-Mediated Complement Resistance and Design Novel Therapeutics

2017-03-23

Gulati S, Schoenhofen IC, Whitfield DM, Cox AD, Li J, St Michael F, Vinogradov EV, Stupak J, Zheng B, Ohnishi M, Unemo M, Lewis LA, Taylor RE, Landig CS, Diaz S, Reed GW, Varki A, Rice PA, Ram S. PLoS Pathog. 2015 Dec 2;11(12):e1005290. doi: 10.1371/journal.ppat.1005290. PMID:...

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Hepatocyte Heparan Sulfate Is Required for Adeno-Associated Virus 2 but Dispensable for Adenovirus 5 Liver Transduction In Vivo

2017-03-23

Zaiss AK, Foley EM, Lawrence R, Schneider LS, Hoveida H, Secrest P, Catapang AB, Yamaguchi Y, Alemany R, Shayakhmetov DM, Esko JD, Herschman HR. J Virol. 2015 Oct 21;90(1):412-20. doi: 10.1128/JVI.01939-15. PMID: 26491162 Abstract Adeno-associated virus 2 (AAV2) and adenovirus 5 (Ad5) are promising gene therapy vectors. Both display...

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Host and pathogen hyaluronan signal through human siglec-9 to suppress neutrophil activation

2017-03-23

Secundino I, Lizcano A, Roupé KM, Wang X, Cole JN, Olson J, Ali SR, Dahesh S, Amayreh LK, Henningham A, Varki A, Nizet V. J Mol Med (Berl). 2016 Feb;94(2):219-33. doi: 10.1007/s00109-015-1341-8. PMID: 26411873 Abstract Inhibitory CD33-related Siglec receptors regulate immune cell activation upon engaging ubiquitous sialic acids (Sias)...

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Heparan Sulfate Modulates Neutrophil and Endothelial Function in Antibacterial Innate Immunity

2017-03-23

Xu D, Olson J, Cole JN, van Wijk XM, Brinkmann V, Zychlinsky A, Nizet V, Esko JD, Chang YC. Infect Immun. 2015 Sep;83(9):3648-56. doi: 10.1128/IAI.00545-15. PMID: 26150541 Abstract Recently, we showed that endothelial heparan sulfate facilitates entry of a bacterial pathogen into the central nervous system. Here, we show...

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Sugar-coated sperm: Unraveling the functions of the mammalian sperm glycocalyx

2017-03-23

Tecle E, Gagneux P. Mol Reprod Dev. 2015 Sep;82(9):635-50. doi: 10.1002/mrd.22500. Review. PMID: 26061344 Abstract Mammalian spermatozoa are coated with a thick glycocalyx that is assembled during sperm development, maturation, and upon contact with seminal fluid. The sperm glycocalyx is critical for sperm survival in the female reproductive tract...

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A red meat-derived glycan promotes inflammation and cancer progression

2017-03-23

Samraj AN, Pearce OM, Läubli H, Crittenden AN, Bergfeld AK, Banda K, Gregg CJ, Bingman AE, Secrest P, Diaz SL, Varki NM, Varki A. Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):542-7. doi: 10.1073/pnas.1417508112. PMID: 25548184 Abstract A well known, epidemiologically reproducible risk factor for human carcinomas...

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Oral streptococci utilize a Siglec-like domain of serine-rich repeat adhesins to preferentially target platelet sialoglycans in human blood

2017-03-23

Deng L, Bensing BA, Thamadilok S, Yu H, Lau K, Chen X, Ruhl S, Sullam PM, Varki A. PLoS Pathog. 2014 Dec 4;10(12):e1004540. doi: 10.1371/journal.ppat.1004540. PMID: 25474103 Abstract Damaged cardiac valves attract blood-borne bacteria, and infective endocarditis is often caused by viridans group streptococci. While such bacteria use multiple...

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Loss of function in heparan sulfate elongation genes EXT1 and EXT 2 results in improved nitric oxide bioavailability and endothelial function

2017-03-23

Mooij HL, Cabrales P, Bernelot Moens SJ, Xu D, Udayappan SD, Tsai AG, van der Sande MA, de Groot E, Intaglietta M, Kastelein JJ, Dallinga-Thie GM, Esko JD, Stroes ES, Nieuwdorp M. J Am Heart Assoc. 2014 Dec 2;3(6):e001274. doi: 10.1161/JAHA.114.001274. PMID: 25468659 Abstract BACKGROUND: Heparanase is the major enzyme...

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Toll-like receptor 3 activation is required for normal skin barrier repair following UV damage

2017-03-23

Borkowski AW, Kuo IH, Bernard JJ, Yoshida T, Williams MR, Hung NJ, Yu BD, Beck LA, Gallo RL. J Invest Dermatol. 2015 Feb;135(2):569-78. doi: 10.1038/jid.2014.354. PMID: 25118157 Abstract UV damage to the skin leads to the release of noncoding RNA (ncRNA) from necrotic keratinocytes that activates Toll-like receptor 3...

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Vaccinia virus binds to the scavenger receptor MARCO on the surface of keratinocytes

2017-03-23

MacLeod DT, Nakatsuji T, Wang Z, di Nardo A, Gallo RL. J Invest Dermatol. 2015 Jan;135(1):142-50. doi: 10.1038/jid.2014.330. PMID: 25089661 Abstract Patients with altered skin immunity, such as individuals with atopic dermatitis (AD), can have a life-threatening disruption of the epidermis known as eczema vaccinatum after vaccinia virus (VV)...

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Subterfuge and sabotage: evasion of host innate defenses by invasive gram-positive bacterial pathogens

2017-03-23

Okumura CY, Nizet V. Annu Rev Microbiol. 2014;68:439-58. doi: 10.1146/annurev-micro-092412-155711. Review. PMID: 25002085 Abstract The development of a severe invasive bacterial infection in an otherwise healthy individual is one of the most striking and fascinating aspects of human medicine. A small cadre of gram-positive pathogens of the genera Streptococcus...

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The classical lancefield antigen of group a Streptococcus is a virulence determinant with implications for vaccine design

2017-03-23

van Sorge NM, Cole JN, Kuipers K, Henningham A, Aziz RK, Kasirer-Friede A, Lin L, Berends ET, Davies MR, Dougan G, Zhang F, Dahesh S, Shaw L, Gin J, Cunningham M, Merriman JA, Hütter J, Lepenies B, Rooijakkers SH, Malley R, Walker MJ, Shattil SJ,...

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Endothelial and leukocyte heparan sulfates regulate the development of allergen-induced airway remodeling in a mouse model

2017-03-23

Ge XN, Ha SG, Rao A, Greenberg YG, Rushdi MN, Esko JD, Rao SP, Sriramarao P. Glycobiology. 2014 Aug;24(8):715-27. doi: 10.1093/glycob/cwu035. PMID: 24794009 Abstract Heparan sulfate (HS) proteoglycans (HSPGs) participate in several aspects of inflammation because of their ability to bind to growth factors, chemokines, interleukins and extracellular matrix...

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A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

2017-03-23

McBride R, Paulson JC, de Vries RP. J Vis Exp. 2016 May 29;(111). doi: 10.3791/53847. PMID: 27284789 Abstract Influenza A virus (IAV) hemagglutinins recognize sialic acids on the cell surface as functional receptors to gain entry into cells. Wild waterfowl are the natural reservoir for IAV, but IAV can...

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Gangliosides of the Vertebrate Nervous System

2017-03-23

Schnaar RL. J Mol Biol. 2016 Aug 14;428(16):3325-36. doi: 10.1016/j.jmb.2016.05.020. Review. PMID: 27261254 Abstract Gangliosides, sialylated glycosphingolipids, found on all vertebrate cells and tissues, are major molecular determinants on the surfaces of vertebrate nerve cells. Composed of a sialylated glycan attached to a ceramide lipid, the same four structures-GM1,...

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Mucins and Their Sugars. Critical Mediators of Hyperreactivity and Inflammation

2017-03-23

Raclawska DS, Ttofali F, Fletcher AA, Harper DN, Bochner BS, Janssen WJ, Evans CM. Ann Am Thorac Soc. 2016 Mar;13 Suppl 1:S98-9. doi: 10.1513/AnnalsATS.201511-743MG. PMID: 27027967 Abstract Excessive mucus causes severe airflow obstruction in fatal asthma. It is also present in mild to moderate disease, but is poorly understood...

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Glycobiology simplified: diverse roles of glycan recognition in inflammation

2017-03-23

Schnaar RL. J Leukoc Biol. 2016 Jun;99(6):825-38. doi: 10.1189/jlb.3RI0116-021R. Review. PMID: 27004978 Abstract Glycans and complementary glycan-binding proteins are essential components in the language of cell-cell interactions in immunity. The study of glycan function is the purview of glycobiology, which has often been presented as an unusually complex discipline....

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"Siglec"ting the allergic response for therapeutic targeting

2017-03-23

Bochner BS. Glycobiology. 2016 Jun;26(6):546-52. doi: 10.1093/glycob/cww024. Review. PMID: 26911285 Abstract As a physician-scientist, I have pursued research related to translational immunology with the goal of improving our ability to diagnose and treat allergic, immunologic and other diseases involving eosinophils, basophils and mast cells. We have tried to delineate...

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Regulation of airway inflammation by Siglec-8 and Siglec-9 sialoglycan ligand expression

2017-03-23

Schleimer RP, Schnaar RL, Bochner BS. Curr Opin Allergy Clin Immunol. 2016 Feb;16(1):24-30. doi: 10.1097/ACI.0000000000000234. Review. PMID: 26694037 Abstract PURPOSE OF REVIEW: Inflammatory cells involved in the allergic response, including eosinophils, mast cells, basophils, and neutrophils, express sialoglycan-binding proteins such as Siglec-8 and Siglec-9, which inhibit cell function and survival....

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Therapeutic Targeting of Siglecs using Antibody- and Glycan-Based Approaches

2017-03-23

Angata T, Nycholat CM, Macauley MS. Trends Pharmacol Sci. 2015 Oct;36(10):645-60. doi: 10.1016/j.tips.2015.06.008. Review. PMID: 26435210 Abstract The sialic acid-binding immunoglobulin-like lectins (Siglecs) are a family of immunomodulatory receptors whose functions are regulated by their glycan ligands. Siglecs are attractive therapeutic targets because of their cell type-specific expression pattern,...

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Mouse Siglec-1 Mediates trans-Infection of Surface-bound Murine Leukemia Virus in a Sialic Acid N-Acyl Side Chain-dependent Manner

2017-03-23

Erikson E, Wratil PR, Frank M, Ambiel I, Pahnke K, Pino M, Azadi P, Izquierdo-Useros N, Martinez-Picado J, Meier C, Schnaar RL, Crocker PR, Reutter W, Keppler OT. J Biol Chem. 2015 Nov 6;290(45):27345-59. doi: 10.1074/jbc.M115.681338. PMID: 26370074 Abstract Siglec-1 (sialoadhesin, CD169) is a surface receptor on human cells...

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Novel Therapies for Eosinophilic Disorders

2017-03-23

Bochner BS.Immunol Allergy Clin North Am. 2015 Aug;35(3):577-98. doi: 10.1016/j.iac.2015.05.007. Review. PMID: 26209901 Abstract Current therapies for eosinophilic disorders are limited. Most treatment approaches remain empirical, are not supported by data from controlled clinical trials, involve the off-label use of agents developed for treatment of other diseases, and...

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B7-H3 Promotes Pathogenesis of Autoimmune Disease and Inflammation by Regulating the Activity of Different T Cell Subsets

2017-03-23

Luo L, Zhu G, Xu H, Yao S, Zhou G, Zhu Y, Tamada K, Huang L, Flies AD, Broadwater M, Ruff W, van Deursen JM, Melero I, Zhu Z, Chen L. PLoS One. 2015 Jun 11;10(6):e0130126. doi: 10.1371/journal.pone.0130126. PMID: 26065426 Abstract B7-H3 is a cell surface molecule in the...

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Efficient Mapping of Sulfated Glycotopes by Negative Ion Mode nanoLC-MS/MS-Based Sulfoglycomic Analysis of Permethylated Glycans

2017-03-23

Cheng CW, Chou CC, Hsieh HW, Tu Z, Lin CH, Nycholat C, Fukuda M, Khoo KH. Anal Chem. 2015 Jun 16;87(12):6380-8. doi: 10.1021/acs.analchem.5b01409. PMID: 26016788 Abstract We have previously developed the enabling techniques for sulfoglycomics based on mass spectrometry (MS) analysis of permethylated glycans, which preserves the attractive features...

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The Role of Vitamin D in Pediatric Asthma

2017-03-23

Bantz SK, Zhu Z, Zheng T. Ann Pediatr Child Health. 2015;3(1). pii: 1032. PMID: 25938135 Abstract The detrimental effects of vitamin D deficiency in pediatrics have become increasingly apparent and extend beyond skeletal health. Unfortunately, vitamin D deficiency is highly prevalent in atopic pediatric patients, in whom it may...

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Reply: To PMID 25497369

2017-03-23

Bochner BS, Kiwamoto T, Katoh T, Zhu Z, Tiemeyer M. J Allergy Clin Immunol. 2015 Jun;135(6):1662-3. doi: 10.1016/j.jaci.2015.02.028. No abstract available. PMID: 25863979 Abstract

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Endogenous airway mucins carry glycans that bind Siglec-F and induce eosinophil apoptosis

2017-03-23

Kiwamoto T, Katoh T, Evans CM, Janssen WJ, Brummet ME, Hudson SA, Zhu Z, Tiemeyer M, Bochner BS. J Allergy Clin Immunol. 2015 May;135(5):1329-40.e1-9. doi: 10.1016/j.jaci.2014.10.027. PMID: 25497369 Abstract BACKGROUND: Sialic acid-binding, immunoglobulin-like lectin (Siglec) F is a glycan-binding protein selectively expressed on mouse eosinophils. Its engagement induces apoptosis, suggesting...

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Systemic blockade of sialylation in mice with a global inhibitor of sialyltransferases

2017-03-23

Macauley MS, Arlian BM, Rillahan CD, Pang PC, Bortell N, Marcondes MC, Haslam SM, Dell A, Paulson JC. J Biol Chem. 2014 Dec 19;289(51):35149-58. doi: 10.1074/jbc.M114.606517. PMID: 25368325 Abstract Sialic acid terminates glycans of glycoproteins and glycolipids that play numerous biological roles in health and disease. Although genetic tools...

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Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation

2017-03-23

Fang P, Zhou L, Zhou Y, Kolls JK, Zheng T, Zhu Z. PLoS One. 2014 Sep 25;9(9):e107454. doi: 10.1371/journal.pone.0107454. PMID: 25254361 Abstract IL-22 is a Th17/Th22 cytokine that is increased in asthma. However, recent animal studies showed controversial findings in the effects of IL-22 in allergic asthma. To determine...

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Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing

2017-03-23

Fang P, Zhou L, Zhou Y, Kolls JK, Zheng T, Zhu Z. PLoS One. 2014 Sep 25;9(9):e107454. doi: 10.1371/journal.pone.0107454. PMID: 25254361 Abstract IL-22 is a Th17/Th22 cytokine that is increased in asthma. However, recent animal studies showed controversial findings in the effects of IL-22 in allergic asthma. To determine...

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TRPA1-dependent pruritus in IL-13-induced chronic atopic dermatitis

2017-03-23

Oh MH, Oh SY, Lu J, Lou H, Myers AC, Zhu Z, Zheng T. J Immunol. 2013 Dec 1;191(11):5371-82. doi: 10.4049/jimmunol.1300300. PMID: 24140646 Abstract Chronic debilitating pruritus is a cardinal feature of atopic dermatitis (AD). Little is known about the underlying mechanisms. Antihistamines lack efficacy in treating itch in...

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Nutrient regulation of gene expression by O-GlcNAcylation of chromatin

2017-03-23

Hardivillé S, Hart GW. Curr Opin Chem Biol. 2016 Aug;33:88-94. doi: 10.1016/j.cbpa.2016.06.005. Review. PMID: 27322399 Abstract O-GlcNAcylation is a dynamic post-translational modification that is responsive to nutrient availably via the hexosamine biosynthetic pathway and its endproduct UDP-GlcNAc. O-GlcNAcylation serves as a nutrient sensor to regulate the activities of many...

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Hijacking the Hexosamine Biosynthetic Pathway to Promote EMT-Mediated Neoplastic Phenotypes

2017-03-23

Taparra K, Tran PT, Zachara NE. Front Oncol. 2016 Apr 18;6:85. doi: 10.3389/fonc.2016.00085. Review. PMID: 27148477 Abstract The epithelial-mesenchymal transition (EMT) is a highly conserved program necessary for orchestrating distant cell migration during embryonic development. Multiple studies in cancer have demonstrated a critical role for EMT during the initial...

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The Translational Repressor 4E-BP1 Contributes to Diabetes-Induced Visual Dysfunction

2017-03-23

Miller WP, Mihailescu ML, Yang C, Barber AJ, Kimball SR, Jefferson LS, Dennis MD. Invest Ophthalmol Vis Sci. 2016 Mar;57(3):1327-37. doi: 10.1167/iovs.15-18719. PMID: 26998719 Abstract PURPOSE: The translational repressor 4E-BP1 interacts with the mRNA cap-binding protein eIF4E and thereby promotes cap-independent translation of mRNAs encoding proteins that contribute to diabetic...

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The nutrient sensor OGT in PVN neurons regulates feeding

2017-03-23

Lagerlöf O, Slocomb JE, Hong I, Aponte Y, Blackshaw S, Hart GW, Huganir RL. Science. 2016 Mar 18;351(6279):1293-6. doi: 10.1126/science.aad5494. PMID: 26989246 Abstract Maintaining energy homeostasis is crucial for the survival and health of organisms. The brain regulates feeding by responding to dietary factors and metabolic signals from peripheral...

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Mass Spectrometry-Based Quantitative O-GlcNAcomic Analysis

2017-03-23

Ma J, Hart GW. Methods Mol Biol. 2016;1410:91-103. doi: 10.1007/978-1-4939-3524-6_6. PMID: 26867740 Abstract The dynamic co- and post-translational modification (PTM) of proteins, O-linked β-D-N-acetylglucosamine modification (O-GlcNAcylation) of serine/threonine residues is critical in many cellular processes, contributing to multiple physiological and pathological events. The term "O-GlcNAcome" refers to not only...

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Glucosamine induces REDD1 to suppress insulin action in retinal Müller cells

2017-03-23

Moore JA, Miller WP, Dennis MD. Cell Signal. 2016 May;28(5):384-90. doi: 10.1016/j.cellsig.2016.01.017. PMID: 26852666 Abstract Resistance to insulin action is a key cause of diabetic complications, yet much remains unknown about the molecular mechanisms that contribute to the defect. Glucose-induced insulin resistance in peripheral tissues such as the retina...

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MRMPlus: an open source quality control and assessment tool for SRM/MRM assay development

2017-03-23

Aiyetan P, Thomas SN, Zhang Z, Zhang H. BMC Bioinformatics. 2015 Dec 12;16:411. doi: 10.1186/s12859-015-0838-z. PMID: 26652794 Abstract BACKGROUND: Selected and multiple reaction monitoring involves monitoring a multiplexed assay of proteotypic peptides and associated transitions in mass spectrometry runs. To describe peptide and associated transitions as stable, quantifiable, and reproducible...

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QUANTITY: An Isobaric Tag for Quantitative Glycomics

2017-03-23

Yang S, Wang M, Chen L, Yin B, Song G, Turko IV, Phinney KW, Betenbaugh MJ, Zhang H, Li S. Sci Rep. 2015 Nov 30;5:17585. doi: 10.1038/srep17585. PMID: 26616285 Abstract Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics--analysis of glycans at global level--however,...

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Identification and Validation of Atypical N-Glycosylation Sites

2017-03-23

Sun S, Zhang H. Anal Chem. 2015 Dec 15;87(24):11948-51. doi: 10.1021/acs.analchem.5b03886. PMID: 26593774 Abstract It is well-known that N-linked glycans usually attach to asparagine residues in the N-X-S/T motifs of proteins. However, accumulating evidence indicates that N-glycosylation could also possibly occur at other atypical motifs. In this study, we...

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Transcriptional Landscape of Cardiomyocyte Maturation

2017-03-23

Uosaki H, Cahan P, Lee DI, Wang S, Miyamoto M, Fernandez L, Kass DA, Kwon C. Cell Rep. 2015 Nov 24;13(8):1705-16. doi: 10.1016/j.celrep.2015.10.032. PMID: 26586429 Abstract Decades of progress in developmental cardiology has advanced our understanding of the early aspects of heart development, including cardiomyocyte (CM) differentiation. However, control...

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CD36 Is a Matrix Metalloproteinase-9 Substrate That Stimulates Neutrophil Apoptosis and Removal During Cardiac Remodeling

2017-03-23

DeLeon-Pennell KY, Tian Y, Zhang B, Cates CA, Iyer RP, Cannon P, Shah P, Aiyetan P, Halade GV, Ma Y, Flynn E, Zhang Z, Jin YF, Zhang H, Lindsey ML. Circ Cardiovasc Genet. 2016 Feb;9(1):14-25. doi: 10.1161/CIRCGENETICS.115.001249. PMID: 26578544 Abstract BACKGROUND: After myocardial infarction, the left ventricle undergoes a wound...

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Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides

2017-03-23

Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Höti N, Zhang Z, Chan DW, Zhang H. Nat Biotechnol. 2016 Jan;34(1):84-8. doi: 10.1038/nbt.3403. PMID: 26571101 Abstract Comprehensive characterization of protein glycosylation is critical for understanding the structure and function of glycoproteins. However,...

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Multiplexed Targeted Mass Spectrometry-Based Assays for the Quantification of N-Linked Glycosite-Containing Peptides in Serum

2017-03-23

Thomas SN, Harlan R, Chen J, Aiyetan P, Liu Y, Sokoll LJ, Aebersold R, Chan DW, Zhang H. Anal Chem. 2015 Nov 3;87(21):10830-8. doi: 10.1021/acs.analchem.5b02063. PMID: 26451657 Abstract Protein glycosylation is one of the most common protein modifications, and the quantitative analysis of glycoproteins has the potential to reveal...

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O-GlcNAcomic Profiling Identifies Widespread O-Linked β-N-Acetylglucosamine Modification (O-GlcNAcylation) in Oxidative Phosphorylation System Regulating Cardiac Mitochondrial Function

2017-03-23

Ma J, Liu T, Wei AC, Banerjee P, O'Rourke B, Hart GW. J Biol Chem. 2015 Dec 4;290(49):29141-53. doi: 10.1074/jbc.M115.691741. PMID: 26446791 Abstract Dynamic cycling of O-linked β-N-acetylglucosamine (O-GlcNAc) on nucleocytoplasmic proteins serves as a nutrient sensor to regulate numerous biological processes. However, mitochondrial protein O-GlcNAcylation and its effects...

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Elucidation of the CHO Super-Ome (CHO-SO) by Proteoinformatics

2017-03-23

Kumar A, Baycin-Hizal D, Wolozny D, Pedersen LE, Lewis NE, Heffner K, Chaerkady R, Cole RN, Shiloach J, Zhang H, Bowen MA, Betenbaugh MJ. J Proteome Res. 2015 Nov 6;14(11):4687-703. doi: 10.1021/acs.jproteome.5b00588. PMID: 26418914 Abstract Chinese hamster ovary (CHO) cells are the preferred host cell line for manufacturing a...

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Chemoenzymatic method for glycomics: Isolation, identification, and quantitation

2017-03-23

Yang S, Rubin A, Eshghi ST, Zhang H. Proteomics. 2016 Jan;16(2):241-56. doi: 10.1002/pmic.201500266. Review. PMID: 26390280 Abstract Over the past decade, considerable progress has been made with respect to the analytical methods for analysis of glycans from biological sources. Regardless of the specific methods that are used, glycan analysis...

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Integrated glycoprotein immobilization method for glycopeptide and glycan analysis of cardiac hypertrophy

2017-03-23

Yang S, Mishra S, Chen L, Zhou JY, Chan DW, Chatterjee S, Zhang H. Anal Chem. 2015 Oct 6;87(19):9671-8. doi: 10.1021/acs.analchem.5b01663. PMID: 26378618 Abstract Post-translational modifications of proteins can have a major role in disease initiation and progression. Incredible efforts have recently been made to study the regulation of...

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Identification and biological consequences of the O-GlcNAc modification of the human innate immune receptor, Nod2

2017-03-23

Hou CW, Mohanan V, Zachara NE, Grimes CL. Glycobiology. 2016 Jan;26(1):13-8. doi: 10.1093/glycob/cwv076. PMID: 26369908 Abstract Nucleotide-binding oligomerization domain 2 (Nod2) is an intracellular receptor that can sense the bacterial peptidoglycan component, muramyl dipeptide. Upon activation, Nod2 induces the production of various inflammatory molecules such as cytokines and chemokines....

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Dual Labeling Biotin Switch Assay to Reduce Bias Derived From Different Cysteine Subpopulations: A Method to Maximize S-Nitrosylation Detection

2017-03-23

Chung HS, Murray CI, Venkatraman V, Crowgey EL, Rainer PP, Cole RN, Bomgarden RD, Rogers JC, Balkan W, Hare JM, Kass DA, Van Eyk JE. Circ Res. 2015 Oct 23;117(10):846-57. doi: 10.1161/CIRCRESAHA.115.307336. PMID: 26338901 Abstract RATIONALE: S-nitrosylation (SNO), an oxidative post-translational modification of cysteine residues, responds to changes in the...

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Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation

2017-03-23

Shah P, Wang X, Yang W, Toghi Eshghi S, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A, Zhang H. Mol Cell Proteomics. 2015 Oct;14(10):2753-63. doi: 10.1074/mcp.M115.047928. PMID: 26256267 Abstract Prostate cancer is the most common cancer among men in the U.S. and worldwide, and androgen-deprivation...

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Prevention of cardiac hypertrophy by the use of a glycosphingolipid synthesis inhibitor in ApoE-/- mice

2017-03-23

Mishra S, Bedja D, Amuzie C, Avolio A, Chatterjee S. Biochem Biophys Res Commun. 2015 Sep 11;465(1):159-64. doi: 10.1016/j.bbrc.2015.07.159. PMID: 26253472 Abstract ApoE-/- mice fed a high fat and high cholesterol (HFHC) diet (20% fat and 1.25% cholesterol) from 12 weeks of age to 36 weeks revealed an age-dependent...

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Improved intervention of atherosclerosis and cardiac hypertrophy through biodegradable polymer-encapsulated delivery of glycosphingolipid inhibitor

2017-03-23

Mishra S, Bedja D, Amuzie C, Foss CA, Pomper MG, Bhattacharya R, Yarema KJ, Chatterjee S. Biomaterials. 2015 Sep;64:125-35. doi: 10.1016/j.biomaterials.2015.06.001. PMID: 26111596 Abstract D-Threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP), a glycosphingolipid synthesis inhibitor, holds promise for the treatment of atherosclerosis and cardiac hypertrophy but rapid in vivo clearance has severely hindered translation...

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Removal of Abnormal Myofilament O-GlcNAcylation Restores Ca2+ Sensitivity in Diabetic Cardiac Muscle

2017-03-23

Ramirez-Correa GA, Ma J, Slawson C, Zeidan Q, Lugo-Fagundo NS, Xu M, Shen X, Gao WD, Caceres V, Chakir K, DeVine L, Cole RN, Marchionni L, Paolocci N, Hart GW, Murphy AM. Diabetes. 2015 Oct;64(10):3573-87. doi: 10.2337/db14-1107. PMID: 26109417 Abstract Contractile dysfunction and increased deposition of O-linked β-N-acetyl-d-glucosamine (O-GlcNAc)...

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Large-Scale Measurement of Absolute Protein Glycosylation Stoichiometry

2017-03-23

Sun S, Zhang H. Anal Chem. 2015 Jul 7;87(13):6479-82. doi: 10.1021/acs.analchem.5b01679. PMID: 26066578 Abstract Protein glycosylation is one of the most important protein modifications. Glycosylation site occupancy alteration has been implicated in human diseases and cancers. However, current glycoproteomic methods focus on the identification and quantification of glycosylated peptides...

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Structures of Capsular Polysaccharide Serotypes 35F and 35C of Streptococcus pneumoniae Determined by Nuclear Magnetic Resonance and Their Relation to Other Cross-Reactive Serotypes

2017-03-23

Bush CA, Cisar JO, Yang J. J Bacteriol. 2015 Sep;197(17):2762-9. doi: 10.1128/JB.00207-15. PMID: 26055112 Abstract The structures of Streptococcus pneumoniae capsular polysaccharides (CPSs) are essential for defining the antigenic as well as genetic relationships between CPS serotypes. The four serotypes that comprise CPS serogroup 35 (i.e., types 35F, 35A,...

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Correction: Overexpression of periostin in stroma positively associated with aggressive prostate cancer

2017-03-23

Tian Y, Choi CH, Li QK, Rahmatpanah FB, Chen X, Kim SR, Veltri R, Chia D, Zhang Z, Mercola D, Zhang H. PLoS One. 2015 Jun 4;10(6):e0130333. doi: 10.1371/journal.pone.0130333. No abstract available. PMID: 26043260  

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Post-translational O-GlcNAcylation is essential for nuclear pore integrity and maintenance of the pore selectivity filter

2017-03-23

Zhu Y, Liu TW, Madden Z, Yuzwa SA, Murray K, Cecioni S, Zachara N, Vocadlo DJ. J Mol Cell Biol. 2016 Feb;8(1):2-16. doi: 10.1093/jmcb/mjv033. PMID: 26031751 Abstract O-glycosylation of the nuclear pore complex (NPC) by O-linked N-acetylglucosamine (O-GlcNAc) is conserved within metazoans. Many nucleoporins (Nups) comprising the NPC are...

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Application of quantitative proteomics to the integrated analysis of the ubiquitylated and global proteomes of xenograft tumor tissues

2017-03-23

Thomas SN, Zhang H, Cotter RJ. Clin Proteomics. 2015 May 20;12(1):14. doi: 10.1186/s12014-015-9086-5. PMID: 26019700 Abstract BACKGROUND: Post-translational modification by ubiquitin is a fundamental regulatory mechanism that is implicated in many cellular processes including the cell cycle, apoptosis, cell adhesion, angiogenesis, and tumor growth. The low stoichiometry of ubiquitylation presents...

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Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells

2017-03-23

Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H. Clin Proteomics. 2015 Apr 11;12(1):11. doi: 10.1186/s12014-015-9083-8. PMID: 25987888 Abstract In this study, we investigated the use of metabolic oligosaccharide engineering and bio-orthogonal ligation reactions combined with lectin microarray...

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GPQuest: A Spectral Library Matching Algorithm for Site-Specific Assignment of Tandem Mass Spectra to Intact N-glycopeptides

2017-03-23

Toghi Eshghi S, Shah P, Yang W, Li X, Zhang H. Anal Chem. 2015;87(10):5181-8. doi: 10.1021/acs.analchem.5b00024. PMID: 25945896 Abstract Glycoprotein changes occur in not only protein abundance but also the occupancy of each glycosylation site by different glycoforms during biological or pathological processes. Recent advances in mass spectrometry instrumentation...

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Prevention of PKG1α oxidation augments cardioprotection in the stressed heart

2017-03-23

Nakamura T, Ranek MJ, Lee DI, Shalkey Hahn V, Kim C, Eaton P, Kass DA. J Clin Invest. 2015 Jun;125(6):2468-72. doi: 10.1172/JCI80275. PMID: 25938783 Abstract The cGMP-dependent protein kinase-1α (PKG1α) transduces NO and natriuretic peptide signaling; therefore, PKG1α activation can benefit the failing heart. Disease modifiers such as oxidative...

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Diabetes-associated dysregulation of O-GlcNAcylation in rat cardiac mitochondria

2017-03-23

Banerjee PS, Ma J, Hart GW. Proc Natl Acad Sci U S A. 2015 May 12;112(19):6050-5. doi: 10.1073/pnas.1424017112. PMID: 25918408 Abstract Elevated mitochondrial O-GlcNAcylation caused by hyperglycemia, as occurs in diabetes, significantly contributes to mitochondrial dysfunction and to diabetic cardiomyopathy. However, little is known about the enzymology of mitochondrial...

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Molecular imaging of inflammation in the ApoE -/- mouse model of atherosclerosis with IodoDPA

2017-03-23

Foss CA, Bedja D, Mease RC, Wang H, Kass DA, Chatterjee S, Pomper MG. Biochem Biophys Res Commun. 2015 May 22;461(1):70-5. doi: 10.1016/j.bbrc.2015.03.171. PMID: 25858322 Abstract BACKGROUND: Atherosclerosis is a common and serious vascular disease predisposing individuals to myocardial infarction and stroke. Intravascular plaques, the pathologic lesions of atherosclerosis, are...

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Identification of glycoproteins containing specific glycans using a lectin-chemical method

2017-03-23

Li Y, Shah P, De Marzo AM, Van Eyk JE, Li Q, Chan DW, Zhang H. Anal Chem. 2015;87(9):4683-7. doi: 10.1021/ac504304v. PMID: 25837443 Abstract Glycosylation is one of the most common protein modifications. Each glycoprotein can be glycosylated at multiple glycosites, and each glycosites can be modified by different...

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Phosphodiesterase 9A controls nitric-oxide-independent cGMP and hypertrophic heart disease

2017-03-23

Lee DI, Zhu G, Sasaki T, Cho GS, Hamdani N, Holewinski R, Jo SH, Danner T, Zhang M, Rainer PP, Bedja D, Kirk JA, Ranek MJ, Dostmann WR, Kwon C, Margulies KB, Van Eyk JE, Paulus WJ, Takimoto E, Kass DA. Nature. 2015 Mar 26;519(7544):472-6. doi:...

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Overexpression of periostin in stroma positively associated with aggressive prostate cancer

2017-03-23

Tian Y, Choi CH, Li QK, Rahmatpanah FB, Chen X, Kim SR, Veltri R, Chia D, Zhang Z, Mercola D, Zhang H.PLoS One. 2015 Mar 17;10(3):e0121502. doi: 10.1371/journal.pone.0121502. Erratum in: PLoS One. 2015;10(6):e0130333. PMID: 25781169 Abstract BACKGROUND: Periostin is an important extracellular matrix protein involved in cell development and...

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O-linked β-N-acetylglucosamine transferase directs cell proliferation in idiopathic pulmonary arterial hypertension

2017-03-23

Barnes JW, Tian L, Heresi GA, Farver CF, Asosingh K, Comhair SA, Aulak KS, Dweik RA. Circulation. 2015 Apr 7;131(14):1260-8. doi: 10.1161/CIRCULATIONAHA.114.013878. PMID: 25663381 Abstract BACKGROUND: Idiopathic pulmonary arterial hypertension (IPAH) is a cardiopulmonary disease characterized by cellular proliferation and vascular remodeling. A more recently recognized characteristic of the disease...

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Emerging proteomic technologies for elucidating context-dependent cellular signaling events: A big challenge of tiny proportions

2017-03-23

Parker SJ, Raedschelders K, Van Eyk JE. Proteomics. 2015 May;15(9):1486-502. doi: 10.1002/pmic.201400448. Review. PMID: 25545106 Abstract Aberrant cell signaling events either drive or compensate for nearly all pathologies. A thorough description and quantification of maladaptive signaling flux in disease is a critical step in drug development, and complex proteomic...

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Three Decades of Research on O-GlcNAcylation - A Major Nutrient Sensor That Regulates Signaling, Transcription and Cellular Metabolism

2017-03-23

Hart GW. Front Endocrinol (Lausanne). 2014 Oct 27;5:183. doi: 10.3389/fendo.2014.00183. Review. PMID: 25386167 Abstract Even though the dynamic modification of polypeptides by the monosaccharide, O-linked N-acetylglucosamine (O-GlcNAcylation) was discovered over 30 years ago, its physiological significance as a major nutrient sensor that regulates myriad cellular processes has only recently been...

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Parkin-independent mitophagy requires Drp1 and maintains the integrity of mammalian heart and brain

2017-03-23

Kageyama Y, Hoshijima M, Seo K, Bedja D, Sysa-Shah P, Andrabi SA, Chen W, Höke A, Dawson VL, Dawson TM, Gabrielson K, Kass DA, Iijima M, Sesaki H. EMBO J. 2014 Dec 1;33(23):2798-813. doi: 10.15252/embj.201488658. PMID: 25349190 Abstract Mitochondrial dynamics and mitophagy have been linked to cardiovascular and neurodegenerative...

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Minireview series on the thirtieth anniversary of research on O-GlcNAcylation of nuclear and cytoplasmic proteins: Nutrient regulation of cellular metabolism and physiology by O-GlcNAcylation

2017-03-23

Hart GW. J Biol Chem. 2014 Dec 12;289(50):34422-3. doi: 10.1074/jbc.R114.609776. PMID: 25336646 Abstract The dynamic cycling of N-acetylglucosamine (termed O-GlcNAcylation) on serine or threonine residues of nuclear or cytoplasmic proteins serves as a nutrient sensor, both independently and also via its interplay with other post-translational modifications, to regulate signaling,...

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Glycoproteomic study reveals altered plasma proteins associated with HIV elite suppressors

2017-03-23

Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou JY, Ao M, Moore RD, Jackson JB, Zhang H. Theranostics. 2014 Sep 18;4(12):1153-63. doi: 10.7150/thno.9510. PMID: 25285165 Abstract HIV elite suppressors (ES) or controllers are individuals achieving control of viremia by their natural immunological mechanisms without highly active...

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Tissue proteomics using chemical immobilization and mass spectrometry

2017-03-23

Shah P, Zhang B, Choi C, Yang S, Zhou J, Harlan R, Tian Y, Zhang Z, Chan DW, Zhang H. Anal Biochem. 2015 Jan 15;469:27-33. doi: 10.1016/j.ab.2014.09.017. PMID: 25283129 Abstract Proteomics analysis is important for characterizing tissues to gain biological and pathological insights, which could lead to the identification...

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Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway

2017-03-23

Zhong J, Martinez M, Sengupta S, Lee A, Wu X, Chaerkady R, Chatterjee A, O'Meally RN, Cole RN, Pandey A, Zachara NE. Proteomics. 2015 Jan;15(2-3):591-607. doi: 10.1002/pmic.201400339. PMID: 25263469 Abstract The modification of intracellular proteins by monosaccharides of O-linked β-N-acetylglucosamine (O-GlcNAc) is an essential and dynamic PTM of metazoans....

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Glycoproteins identified from heart failure and treatment models

2017-03-23

Yang S, Chen L, Sun S, Shah P, Yang W, Zhang B, Zhang Z, Chan DW, Kass DA, van Eyk JE, Zhang H. Proteomics. 2015 Jan;15(2-3):567-79. doi: 10.1002/pmic.201400151. PMID: 25141849 Abstract Conduction abnormalities can lead to dyssynchronous contraction, which significantly worsens morbidity and mortality of heart failure. Cardiac resynchronization...

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Nutrient regulation of signaling, transcription, and cell physiology by O-GlcNAcylation

2017-03-23

Hardivillé S, Hart GW. Cell Metab. 2014 Aug 5;20(2):208-13. doi: 10.1016/j.cmet.2014.07.014. Review. PMID: 25100062 Abstract The nutrient sensor, O-linked N-acetylglucosamine (O-GlcNAc), cycles on and off nuclear and cytosolic proteins to regulate many cellular processes, including transcription and signaling. Dysregulated O-GlcNAcylation and its interplay with phosphorylation contribute to the etiology of diabetes,...

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Imaging of N-linked glycans from formalin-fixed paraffin-embedded tissue sections using MALDI mass spectrometry

2017-03-23

Toghi Eshghi S, Yang S, Wang X, Shah P, Li X, Zhang H. ACS Chem Biol. 2014 Sep 19;9(9):2149-56. doi: 10.1021/cb500405h. PMID: 25029481 Abstract Aberrant glycosylation is associated with most of the diseases. Direct imaging and profiling of N-glycans on tissue sections can reveal tissue-specific and/or disease-associated N-glycans, which...

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Chemical structures of Streptococcus pneumoniae capsular polysaccharide type 39 (CPS39), CPS47F, and CPS34 characterized by nuclear magnetic resonance spectroscopy and their relation to CPS10A

2017-03-23

Bush CA, Yang J, Yu B, Cisar JO. J Bacteriol. 2014 Sep;196(18):3271-8. doi: 10.1128/JB.01731-14. PMID: 25002537 Abstract Structural characterization of Streptococcus pneumoniae capsular polysaccharides (CPS) is a prerequisite for unraveling both antigenic and genetic relationships that exist between different serotypes. In the current study, comparative structural studies of S....

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Physiologic and pathophysiologic consequences of altered sialylation and glycosylation on ion channel function

2017-03-23

Baycin-Hizal D, Gottschalk A, Jacobson E, Mai S, Wolozny D, Zhang H, Krag SS, Betenbaugh MJ. Biochem Biophys Res Commun. 2014 Oct 17;453(2):243-53. doi: 10.1016/j.bbrc.2014.06.067. Review. PMID: 24971539 Abstract Voltage-gated ion channels are transmembrane proteins that regulate electrical excitability in cells and are essential components of the electrically active...

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Glycoform analysis of recombinant and human immunodeficiency virus envelope protein gp120 via higher energy collisional dissociation and spectral-aligning strategy

2017-03-23

Yang W, Shah P, Toghi Eshghi S, Yang S, Sun S, Ao M, Rubin A, Jackson JB, Zhang H. Anal Chem. 2014 Jul 15;86(14):6959-67. doi: 10.1021/ac500876p. PMID: 24941220 Abstract Envelope protein gp120 of human immunodeficiency virus (HIV) is armored with a dense glycan shield, which plays critical roles in...

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Cardiac extracellular proteome profiling and membrane topology analysis using glycoproteomics

2017-03-23

Tian Y, Koganti T, Yao Z, Cannon P, Shah P, Pietrovito L, Modesti A, Aiyetan P, DeLeon-Pennell K, Ma Y, Halade GV, Hicks C, Zhang H, Lindsey ML. Proteomics Clin Appl. 2014 Aug;8(7-8):595-602. doi: 10.1002/prca.201400009. PMID: 24920555 Abstract PURPOSE: Extracellular proteins are easily accessible, which presents a subproteome of molecular...

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Overexpression of α (1,6) fucosyltransferase associated with aggressive prostate cancer

2017-03-23

Wang X, Chen J, Li QK, Peskoe SB, Zhang B, Choi C, Platz EA, Zhang H. Glycobiology. 2014 Oct;24(10):935-44. doi: 10.1093/glycob/cwu051. PMID: 24906821 Abstract Aberrant protein glycosylation is known to be associated with the development of cancers. The aberrant glycans are produced by the combined actions of changed glycosylation...

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Precardiac deletion of Numb and Numblike reveals renewal of cardiac progenitors

2017-03-23

Shenje LT, Andersen P, Uosaki H, Fernandez L, Rainer PP, Cho GS, Lee DI, Zhong W, Harvey RP, Kass DA, Kwon C. Elife. 2014 Apr 24;3:e02164. doi: 10.7554/eLife.02164. PMID: 24843018 Abstract Cardiac progenitor cells (CPCs) must control their number and fate to sustain the rapid heart growth during development,...

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Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness

2017-03-23

Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R. Mol Cell Proteomics. 2014 Jul;13(7):1753-68. doi: 10.1074/mcp.M114.038273. PMID: 24741114 Abstract The identification of biomarkers indicating the level of aggressiveness of prostate cancer (PCa) will address the urgent clinical need...

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Inhibition of glycosphingolipid synthesis ameliorates atherosclerosis and arterial stiffness in apolipoprotein E-/- mice and rabbits fed a high-fat and -cholesterol diet

2017-03-23

Chatterjee S, Bedja D, Mishra S, Amuzie C, Avolio A, Kass DA, Berkowitz D, Renehan M. Circulation. 2014 Jun 10;129(23):2403-13. doi: 10.1161/CIRCULATIONAHA.113.007559. PMID: 24710030 Abstract BACKGROUND: Glycosphingolipids, integral components of the cell membrane, have been shown to serve as messengers, transducing growth factor-initiated phenotypes. Here, we have examined whether inhibition...

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The role of O-GlcNAc signaling in the pathogenesis of diabetic retinopathy

2017-03-23

Semba RD, Huang H, Lutty GA, Van Eyk JE, Hart GW. Proteomics Clin Appl. 2014 Apr;8(3-4):218-31. doi: 10.1002/prca.201300076. Review. PMID: 24550151 Abstract Diabetic retinopathy is a leading cause of blindness worldwide. Despite laser and surgical treatments, antiangiogenic and other therapies, and strict metabolic control, many patients progress to visual...

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Epithelium percentage estimation facilitates epithelial quantitative protein measurement in tissue specimens

2017-03-23

Chen J, Toghi Eshghi S, Bova GS, Li QK, Li X, Zhang H. Clin Proteomics. 2013 Dec 1;10(1):18. doi: 10.1186/1559-0275-10-18. PMID: 24289299 Abstract BACKGROUND: The rapid advancement of high-throughput tools for quantitative measurement of proteins has demonstrated the potential for the identification of proteins associated with cancer. However, the quantitative...

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Post-translational modifications: a major focus for the future of proteomics

2017-03-23

Hart GW, Ball LE. Mol Cell Proteomics. 2013 Dec;12(12):3443. doi: 10.1074/mcp.E113.036491. No abstract available. PMID: 24277940

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Inhibition of protein carbamylation in urea solution using ammonium-containing buffers

2017-03-23

Sun S, Zhou JY, Yang W, Zhang H. Anal Biochem. 2014 Feb 1;446:76-81. doi: 10.1016/j.ab.2013.10.024. PMID: 24161613 Abstract Urea solution is one of the most commonly employed protein denaturants for protease digestion in proteomic studies. However, it has long been recognized that urea solution can cause carbamylation at the...

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Nutrient regulation of gene expression by O-GlcNAcylation of chromatin

2017-03-23

Hardivillé S, Hart GW.Curr Opin Chem Biol. 2016 Aug;33:88-94. doi: 10.1016/j.cbpa.2016.06.005. Review. PMID: 27322399 Abstract O-GlcNAcylation is a dynamic post-translational modification that is responsive to nutrient availably via the hexosamine biosynthetic pathway and its endproduct UDP-GlcNAc. O-GlcNAcylation serves as a nutrient sensor to regulate the activities of many...

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Molecular Functions of Glycoconjugates in Autophagy

2017-03-22

Fahie K, Zachara NE. J Mol Biol. 2016 Aug 14;428(16):3305-24. doi: 10.1016/j.jmb.2016.06.011. Review. PMID: 27345664 Abstract Glycoconjugates, glycans, carbohydrates, and sugars: these terms encompass a class of biomolecules that are diverse in both form and function ranging from free oligosaccharides, glycoproteins, and proteoglycans, to glycolipids that make up a...

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Targeted proteomic assays for the verification of global proteomics insights

2017-03-22

Thomas SN, Zhang H. Expert Rev Proteomics. 2016 Sep 1:1-3. PMID: 27565203

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Project 5 Update

2016-12-01

AIM 1: We are revising a manuscript (for Cell Reports) that shows how ADAMTS9 regulates the growth of arteries. Specifically, we show, using a new gene-trap mouse allele, that ADAMTS9 is essential for matrix remodeling in the umbilical cord that is a prerequisite for differentiation...

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An Open Receptor-Binding Cavity of Hemagglutinin-Esterase-Fusion Glycoprotein from Newly-Identified Influenza D Virus: Basis for Its Broad Cell Tropism

2016-02-04

Song, H, Qi, J., Khedri, Z., Diaz, S., Yu, H., Chen, X., Varki, A., Shi, Y., Gao, G.F.: An Open Receptor-Binding Cavity of Hemagglutinin-Esterase-Fusion Glycoprotein from Newly-Identified Influenza D Virus: Basis for Its Broad Cell Tropism. PLoS Pathog. 2016 Jan 27;12(1):e1005411. doi: 10.1371/journal.ppat.1005411. eCollection 2016...

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Symbol Nomenclature for Graphical Representations of Glycans

2016-02-04

Varki, A., Cummings, R.D., Aebi, M., Packer, N.H., Seeberger, P.H., Esko, J.D., Stanley, P., Hart, G., Darvill, A., Kinoshita, T., Prestegard, J.J., Schnaar, R.L., Freeze, H.H., Martin, J.D., Bertozzi, C.R., Etzler, M.S., Frank, M., Vliegenthart, J.F., Lütteke, T., Perez, S., Bolton, E., Rudd, P.,...

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Human-specific Derived Alleles of CD33 and Other Genes Protect Against Post-Reproductive Cognitive Decline

2016-02-04

Schwarz, F., Springer, S., Altheide, T., Varki, N., Gagneux, P., and Varki, A.: Human-specific Derived Alleles of CD33 and Other Genes Protect Against Post-Reproductive Cognitive Decline. 2015. Proc. Nat'l. Acad. Sci. U.S.A. doi: 10.1073/pnas.1517951112.

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Immunomodulatory Activity of Extracellular Hsp70 Mediated Via Paired Receptors Siglec-5 and Siglec-14

2016-02-04

Fong, J.J., Sreedhara, K., Deng, L., Varki, N., Angata, T., Liu, Q., Nizet, V. and Varki, A.: Immunomodulatory Activity of Extracellular Hsp70 Mediated Via Paired Receptors Siglec-5 and Siglec-14. EMBO J. 2015. 34:2775-2788

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Human Siglec-9 Binds Hyaluronan to Regulate Neutrophil Activation but is Exploited by Bacterial Pathogen Mimicry

2015-09-21

Secunndino, I.,Lizcano, A., Roupe, K.M., Wang, X., Cole, J.N., Olson, J., Ali, S.R., Dahesh, S., Amayreh, L.K., Henningham, A., Varki, A. and Nizet, V.: Human Siglec-9 Binds Hyaluronan to Regulate Neutrophil Activation but is Exploited by Bacterial Pathogen Mimicry. J. Mol. Med. 2015. In press

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The multiple, complex roles of versican and its proteolytic turnover by ADAMTS proteases during embryogenesis

2015-08-28

Nandadasa S, Foulcer S, Apte SS. The multiple, complex roles of versican and its proteolytic turnover by ADAMTS proteases during embryogenesis. Matrix Biol. 2014 Apr;35:34-41. doi: 10.1016/j.matbio.2014.01.005. PMID: 24444773 Abstract Embryonic development is an exceptionally dynamic process, requiring a provisional extracellular matrix that is amenable to rapid...

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Isolation and purification of versican and analysis of versican proteolysis

2015-08-28

Foulcer SJ, Day AJ, Apte SS. Isolation and purification of versican and analysis of versican proteolysis. Methods Mol Biol. 2015;1229:587-604. doi: 10.1007/978-1-4939-1714-3_46. PMID: 25325983 Abstract Versican is a widely distributed chondroitin sulfate proteoglycan that forms large complexes with the glycosaminoglycan hyaluronan (HA). As a consequence of HA...

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Is pulmonary hypertension a metabolic disease?

2015-08-28

Barnes J, Dweik RA. Is pulmonary hypertension a metabolic disease? Am J Respir Crit Care Med. 2014 Nov 1;190(9):973-5. doi: 10.1164/rccm.201409-1702ED. PMID: 25360726 PMCID: PMC4299590

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Volatile organic compounds in bile can diagnose malignant biliary strictures in the setting of pancreatic cancer: a preliminary observation

2015-08-28

Navaneethan U, Parsi MA, Gutierrez NG, Bhatt A, Venkatesh PG, Lourdusamy D, Grove D, Hammel JP, Jang S, Sanaka MR, Stevens T, Vargo JJ,Dweik RA. Volatile organic compounds in bile can diagnose malignant biliary strictures in the setting of pancreatic cancer: a preliminary observation. Gastrointest Endosc. 2014 Dec;80(6):1038-45. doi: 10.1016/j.gie.2014.04.016. PMID: 24929484 Abstract BACKGROUND: Ascertaining the nature of biliary...

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Leptin deficiency recapitulates the histological features of pulmonary arterial hypertension in mice

2015-08-28

Aytekin M, Tonelli AR, Farver CF, Feldstein AE, Dweik RA. Leptin deficiency recapitulates the histological features of pulmonary arterial hypertension in mice. Int J Clin Exp Pathol. 2014 Apr 15;7(5):1935-46. PMID: 24966903 PMCID: PMC4069962 Abstract Leptin is a neuroendocrine peptide released by adipose tissue that enhances metabolism...

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The dietary polysaccharide maltodextrin promotes Salmonella survival and mucosal colonization in mice

2015-08-28

Nickerson KP, Homer CR, Kessler SP, Dixon LJ, Kabi A, Gordon IO, Johnson EE, de la Motte CA, McDonald C. The dietary polysaccharide maltodextrin promotes Salmonella survival and mucosal colonization in mice. PLoS One. 2014 Jul 7;9(7):e101789. doi: 10.1371/journal.pone.0101789. PMID: 25000398 PMCID: PMC4084946 Abstract In the latter half of the 20th century, societal and technological...

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Compartmentalization and Kinetics of Counterbalancing Signals in Cardiac Transplants

2015-08-27

Kaul A, Goparaju S, Dvorina N, Iida S, Keslar K, de la Motte C, Valujskik A, Fairchild R and Baldwin W. Acute and Chronic Rejection: Compartmentalization and Kinetics of Counterbalancing Signals in Cardiac Transplants. Am J Transplant. 15:333-45 (2015) PMID: 25582188 PMCID:PMC4304877 Abstract Acute and chronic rejection...

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Roles of proteoglycans and glycosaminoglycans in wound healing and fibrosis

2015-08-27

Ghatak S, Markwald RR, Hascall VC, Maytin EV, Mack JA, Atanelishvili I, Rodriguez RM, Misra S. Roles of proteoglycans and glycosaminoglycans in wound healing and fibrosis. Int J Cell Biol (2015 accepted).

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Isothiocyanate analogs targeting CD44 receptor as an effective strategy against colon cancer

2015-08-14

Misra S, Ghatak S, Vyas A, O'Brien P, Markwald RR, Khetmalas M, Hascall VC, McCarthy JB, Karamanos NK, Tammi MI, Tammi RH, Prestwitch GD, Padhye S. Isothiocyanate analogs targeting CD44 receptor as an effective strategy against colon cancer. Med Chem Res. 2014 Aug 1;23(8):3836-3851. PMID:...

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Natural antisense transcript for hyaluronan synthase 2 (HAS2-AS1) induces transcription of HAS2 via protein O-GlcNAcylation

2015-08-14

Vigetti D, Deleonibus S, Moretto P, Bowen T, Fischer JW, Grandoch M, Oberhuber A, Love DC, Hanover JA, Cinquetti R, Karousou E, Viola M, D'Angelo ML, Hascall VC, De Luca G, Passi A. Natural antisense transcript for hyaluronan synthase 2 (HAS2-AS1) induces transcription of HAS2...

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Analysis of the heavy-chain modification and TSG-6 activity in pathological hyaluronan matrices

2015-08-14

Lauer ME, Loftis J, de la Motte C, Hascall VC. Analysis of the heavy-chain modification and TSG-6 activity in pathological hyaluronan matrices. Methods Mol Biol. 2015;1229:543-8. doi: 10.1007/978-1-4939-1714-3_42. PMID: 25325979 PMCID: PMC4397960 Abstract During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred...

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Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan

2015-08-14

Lamkin E, Cheng G, Calabro A, Hascall VC, Joo EJ, Li L, Linhardt RJ, Lauer ME. Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan. J Biol Chem. 2015 Feb 20;290(8):5156-66. doi: 10.1074/jbc.M114.636258. PMID: 25561734 PMCID: PMC4335249 Abstract We present data that hyaluronan...

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Interactions between Hyaluronan and Its Receptors (CD44, RHAMM) Regulate the Activities of Inflammation and Cancer

2015-08-14

Misra S, Hascall VC, Markwald RR, Ghatak S. Interactions between Hyaluronan and Its Receptors (CD44, RHAMM) Regulate the Activities of Inflammation and Cancer. Front Immunol. 2015 May 6;6:201. doi: 10.3389/fimmu.2015.00201. PMID: 25999946 PMCID: PMC4422082 Abstract The glycosaminoglycan hyaluronan (HA), a major component of extracellular matrices, and cell...

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Heparin interaction with a receptor on hyperglycemic dividing cells prevents intracellular hyaluronan synthesisand autophagy responses in models of type 1 diabetes

2015-08-14

Wang A, Sankaranarayanan NV, Yanagishita M, Templeton DM, Desai UR, Sugahara K, Wang CP, Hascall VC. Heparin interaction with a receptor on hyperglycemic dividing cells prevents intracellular hyaluronan synthesisand autophagy responses in models of type 1 diabetes. Matrix Biol. 2015 Apr 16. pii: S0945-053X(15)00082-7. doi:...

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Hyaluronan regulates bone morphogenetic protein-7-dependent prevention and reversal of myofibroblast phenotype

2015-08-14

Midgley AC, Duggal L, Jenkins R, Hascall V, Steadman R, Phillips AO, Meran S. Hyaluronan regulates bone morphogenetic protein-7-dependent prevention and reversal of myofibroblast phenotype. J Biol Chem. 2015 May 1;290(18):11218-34. doi: 10.1074/jbc.M114.625939. PMID: 25716319 PMCID: PMC4416830 Abstract Hyaluronan (HA) promotes transforming growth factor (TGF)-β1-driven myofibroblast phenotype....

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Utilization of GAGs/PGs as carriers for targeted therapy delivery

2015-08-14

Misra S, Hascall V, Markwald R, Atanelishvili I, Moreno-Rodriguez, R, Ghatak S. Utilization of GAGs/PGs as carriers for targeted therapy delivery. Int. J. Cell Biol. 2015 (In Press)

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Transforming growth factor-β2 is sequestered in preterm human milk by chondroitin sulfate proteoglycans

2015-08-14

Namachivayam K1, Coffing HP2, Sankaranarayanan NV3, Jin Y3, MohanKumar K1, Frost BL4, Blanco CL5, Patel AL6, Meier PP7, Garzon SA8, Desai UR3,Maheshwari A9. Transforming growth factor-β2 is sequestered in preterm human milk by chondroitin sulfate proteoglycans. Am J Physiol Gastrointest Liver Physiol. 2015 Aug 1;309(3):G171-80....

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Allosteric inhibition of factor XIa. Sulfated non-saccharide glycosaminoglycan mimetics as promising anticoagulants

2015-08-14

Al-Horani RA, Gailani D, Desai UR. Allosteric inhibition of factor XIa. Sulfated non-saccharide glycosaminoglycan mimetics as promising anticoagulants. Thromb Res. 2015 Aug;136(2):379-87. doi: 10.1016/j.thromres.2015.04.017. PMID: 25935648 PMCID: PMC4526391 Abstract Recent development of sulfated non-saccharide glycosaminoglycan mimetics, especially sulfated pentagalloyl glucopyranoside (SPGG), as potent inhibitors of factor XIa...

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Heparin interaction with a receptor on hyperglycemic dividing cells prevents intracellular hyaluronan synthesis and autophagy responses in models of type 1 diabetes

2015-08-14

Wang A, Sankaranarayanan NV, Yanagishita M, Templeton DM, Desai UR, Sugahara K, Wang CP, Hascall VC. Heparin interaction with a receptor on hyperglycemic dividing cells prevents intracellular hyaluronan synthesis and autophagy responses in models of type 1 diabetes. Matrix Biol. 2015 Apr 16. pii: S0945-053X(15)00082-7....

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Chemoenzymatically Prepared Heparan Sulfate Containing Rare 2-O-Sulfonated Glucuronic Acid Residues

2015-08-14

Boothello RS, Sarkar A, Tran VM, Nguyen TK, Sankaranarayanan NV, Mehta AY, Alabbas A, Brown S, Rossi A, Joice AC, Mencio CP, Quintero MV, Kuberan B, Desai UR. Chemoenzymatically Prepared Heparan Sulfate Containing Rare 2-O-Sulfonated Glucuronic Acid Residues. ACS Chem Biol. 2015 Jun 19;10(6):1485-94. doi:...

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Investigation of the heparin-thrombin interaction by dynamic force spectroscopy

2015-08-14

Wang C, Jin Y, Desai UR, Yadavalli VK. Investigation of the heparin-thrombin interaction by dynamic force spectroscopy. Biochim Biophys Acta. 2015 Jun;1850(6):1099-106. doi: 10.1016/j.bbagen.2015.01.016. PMID: 25647100 PMCID: PMC4398634 Abstract BACKGROUND: The interaction between heparin and thrombin is a vital step in the blood (anti)coagulation process. Unraveling the molecular...

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Plasmin regulation through allosteric, sulfated, small molecules

2015-08-14

Al-Horani RA1, Karuturi R2, White DT3, Desai UR4. Plasmin regulation through allosteric, sulfated, small molecules. Molecules. 2015 Jan 5;20(1):608-24. doi: 10.3390/molecules20010608. PMID: 25569517 Abstract Plasmin, a key serine protease, plays a major role in clot lysis and extracellular matrix remodeling. Heparin, a natural polydisperse sulfated glycosaminoglycan, is...

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Preface. Glycosaminoglycans.

2015-08-14

Balagurunathan K, Nakato H, Desai UR. Preface. Glycosaminoglycans. Methods Mol Biol. 2015;1229:v. PMID: 25489643 Abstract Not available

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A simple, general approach of allosteric coagulation enzyme inhibition through monosulfated hydrophobic scaffolds

2015-08-14

Sidhu PS1, Zhou Q2, Desai UR3. A simple, general approach of allosteric coagulation enzyme inhibition through monosulfated hydrophobic scaffolds. Bioorg Med Chem Lett. 2014 Dec 15;24(24):5716-20. doi: 10.1016/j.bmcl.2014.10.059. PMID: 25453807 Abstract Allosteric inhibition of coagulation enzymes offers the advantage of controlled inhibition. In this study, a small...

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Studying glycosaminoglycan-protein interactions using capillary electrophoresis

2015-08-14

Liang A1, Desai UR. Studying glycosaminoglycan-protein interactions using capillary electrophoresis. Methods Mol Biol. 2015;1229:355-75. doi: 10.1007/978-1-4939-1714-3_28. PMID: 25325965 Abstract Methods for studying interactions between glycosaminoglycans (GAGs) and proteins have assumed considerable significance as their biological importance increases. Capillary electrophoresis (CE) is a powerful method to study these...

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Glycosaminoglycan-protein interaction studies using fluorescence spectroscopy

2015-08-14

Boothello RS, Al-Horani RA, Desai UR. Glycosaminoglycan-protein interaction studies using fluorescence spectroscopy. Methods Mol Biol. 2015;1229:335-53. doi: 10.1007/978-1-4939-1714-3_27. PMID: 25325964 Abstract Fluorescence spectroscopy is a quantitative analytical tool that has been extensively used to provide structural and dynamical information on GAG-protein complexes. It possesses major advantages including...

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Designing "high-affinity, high-specificity" glycosaminoglycan sequences through computerized modeling

2015-08-13

Sankaranarayanan NV, Sarkar A, Desai UR, Mosier PD. Designing "high-affinity, high-specificity" glycosaminoglycan sequences through computerized modeling. Methods Mol Biol. 2015;1229:289-314. doi: 10.1007/978-1-4939-1714-3_24. PMID: 25325961 PMCID: PMC4452111. Abstract The prediction of high-affinity and/or high-specificity protein-glycosaminoglycan (GAG) interactions is an inherently difficult task, due to several factors including the...

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Synthesis of glycosaminoglycan mimetics through sulfation of polyphenols

2015-08-13

Al-Horani RA1, Karuturi R, Verespy S 3rd, Desai UR. Synthesis of glycosaminoglycan mimetics through sulfation of polyphenols. Methods Mol Biol. 2015;1229:49-67. doi: 10.1007/978-1-4939-1714-3_7. PMID: 25325944 Abstract In nearly all cases of biological activity of sulfated GAGs, the sulfate group(s) are critical for interacting with target proteins. A...

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Sulfated low molecular weight lignins, allosteric inhibitors of coagulation proteinases via the heparin binding site, significantly alter the active site of thrombin and factor xa compared to heparin

2015-08-13

Henry BL, Desai UR. Sulfated low molecular weight lignins, allosteric inhibitors of coagulation proteinases via the heparin binding site, significantly alter the active site of thrombin and factor xa compared to heparin. Thromb Res. 2014 Nov;134(5):1123-9. doi: 10.1016/j.thromres.2014.08.024. PMID: 25242245 PMCID: PMC4253681 Abstract Sulfated low molecular weight lignins...

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Toward a robust computational screening strategy for identifying glycosaminoglycan sequences that display high specificity for target proteins

2015-08-13

Sankaranarayanan NV, Desai UR. Toward a robust computational screening strategy for identifying glycosaminoglycan sequences that display high specificity for target proteins. Glycobiology. 2014 Dec;24(12):1323-33. doi: 10.1093/glycob/cwu077. PMID: 25049239 PMCID: PMC4296175 Abstract Glycosaminoglycans (GAGs) interact with many proteins to regulate processes such as hemostasis, cell adhesion, growth and...

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Therapeutic effects of cell-permeant peptides that activate G proteins downstream of growth factors

2015-08-13

Ma GS, Aznar N, Kalogriopoulos N, Midde KK, Lopez-Sanchez I, Sato E, Dunkel Y, Gallo RL, Ghosh P. Therapeutic effects of cell-permeant peptides that activate G proteins downstream of growth factors. Proc Natl Acad Sci U S A. 2015 May 19;112(20):E2602-10. doi:10.1073/pnas.1505543112. PMID: 25926659 PMCID:...

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Endogenous intracellular cathelicidin enhances TLR9 activation in dendritic cells and macrophages

2015-08-13

Nakagawa Y, Gallo RL. Endogenous intracellular cathelicidin enhances TLR9 activation in dendritic cells and macrophages. J Immunol. 2015 Feb 1;194(3):1274-84. doi: 10.4049/jimmunol.1402388. PMID: 25548223 PMCID: PMC4297737. Abstract Cathelicidins are a gene family best known for their antimicrobial action, but the diverse mature peptides they encode also have other...

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Innate immunity. Dermal adipocytes protect against invasive Staphylococcus aureus skin infection

2015-08-13

Zhang LJ, Guerrero-Juarez CF, Hata T, Bapat SP, Ramos R, Plikus MV, Gallo RL. Innate immunity. Dermal adipocytes protect against invasive Staphylococcus aureus skin infection. Science. 2015 Jan 2;347(6217):67-71. doi: 10.1126/science.1260972. PMID: 25554785 PMCID: PMC4318537. Abstract Adipocytes have been suggested to be immunologically active, but their role in host...

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Brown fat activation reduces hypercholesterolaemia and protects from atherosclerosis development

2015-08-13

Berbée JF, Boon MR, Khedoe PP, Bartelt A, Schlein C, Worthmann A, Kooijman S, Hoeke G, Mol IM, John C, Jung C, Vazirpanah N, Brouwers LP, Gordts PL, Esko JD, Hiemstra PS, Havekes LM, Scheja L, Heeren J, Rensen PC. Brown fat activation reduces hypercholesterolaemia...

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Siglec receptors impact mammalian lifespan by modulating oxidative stress

2015-08-13

Schwarz F, Pearce OM, Wang X, Samraj AN, Läubli H, Garcia JO, Lin H, Fu X, Garcia-Bingman A, Secrest P, Romanoski CE, Heyser C, Glass CK, Hazen SL, Varki N, Varki A, Gagneux P. Siglec receptors impact mammalian lifespan by modulating oxidative stress. Elife. 2015...

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Glycans and glycan-binding proteins in immune regulation: A concise introduction to glycobiology for the allergist.

2015-02-23

Schnaar RL. (2015) Glycans and glycan-binding proteins in immune regulation: A concise introduction to glycobiology for the allergist. J Allergy Clin Immunol. 2015 Jan 30. pii: S0091-6749(15)00007-X. doi: 10.1016/j.jaci.2014.10.057. [Epub ahead of print] PMID: 25649080. Abstract Cells are endowed with a rich surface coat of glycans that...

Role of siglecs and related glycan-binding proteins in immune responses and immunoregulation.

2015-02-02

Bochner BS, Zimmermann N. (2015) Role of siglecs and related glycan-binding proteins in immune responses and immunoregulation. J Allergy Clin Immunol. 2015 Jan 12. pii: S0091-6749(14)01734-5. doi: 10.1016/j.jaci.2014.11.031. [Epub ahead of print] PMID: 25592986. Abstract Virtually all cells and extracellular material are heavily decorated by various glycans,...

Hyaluronan breakdown contributes to immune defense against group A Streptococcus.

2014-12-21

Schommer NN, Muto J, Nizet V, Gallo RL. (2014) Hyaluronan breakdown contributes to immune defense against group A Streptococcus. J Biol Chem. 289(39):26914-21. PMID: 25122767. PMCID: PMC4175332. Abstract Group A Streptococcus (GAS) commonly infects human skin and occasionally causes severe and life-threatening invasive diseases. The hyaluronan (HA)...

Targeted proteomics: a bridge between discovery and validation

2014-12-20

Harlan R, Zhang H. (2014) Targeted proteomics: a bridge between discovery and validation. Expert Rev Proteomics. 11(6):657-61. PMID: 25348939. Abstract New technologies in mass spectrometry are beginning to mature and show unique advantages for the identification and quantitation of proteins. In recent years, one of the significant...

Soluble Guanylate Cyclase Is Required for Systemic Vasodilation But Not Positive Inotropy Induced by Nitroxyl in the Mouse.

2014-12-07

Zhu G, Groneberg D, Sikka G, Hori D, Ranek MJ, Nakamura T, Takimoto E, Paolocci N, Berkowitz DE, Friebe A, Kass DA.(2014) Soluble Guanylate Cyclase Is Required for Systemic Vasodilation But Not Positive Inotropy Induced by Nitroxyl in the Mouse. Hypertension. 65(2):385-92. PMID: 25452469. PMCID:...

Hyaluronan synthase 2 protects skin fibroblasts against apoptosis induced by environmental stress.

2014-11-26

Wang Y, Lauer ME, Anand S, Mack JA, Maytin EV. (2014) Hyaluronan synthase 2 protects skin fibroblasts against apoptosis induced by environmental stress. J Biol Chem. 289(46):32253-65. PMID: 25266724. PMCID: PMC4231699. Abstract A balanced turnover of dermal fibroblasts is crucial for structural integrity and normal function of...

Improved in-gel reductive Beta-elimination for comprehensive O-linked and sulfo-glycomics by mass spectrometry

2014-11-20

Nix DB, Kumagai T, Katoh T, Tiemeyer M, Aoki K. (2014) Improved in-gel reductive β-elimination for comprehensive O-linked and sulfo-glycomics by mass spectrometry. J Vis Exp.(93):e51840. doi: 10.3791/51840. PMID: 25489664. Abstract Separation of proteins by SDS-PAGE followed by in-gel proteolytic digestion of resolved protein bands has produced...

Stable RAGE-heparan sulfate complexes are essential for signal transduction

2014-11-16

Xu D, Young JH, Krahn JM, Song D, Corbett KD, Chazin WJ, Pedersen LC, Esko JD. (2014) Stable RAGE-heparan sulfate complexes are essential for signal transduction. ACS Chem Biol. 8(7):1611-20. PMID: 23679870. PMCID: PMC3806902. Abstract RAGE (Receptor for Advanced Glycation End-Products) has emerged as a major receptor...

Reducing macrophage proteoglycan sulfation increases atherosclerosis and obesity through enhanced type I interferon signaling

2014-11-04

Gordts PL, Foley EM, Lawrence R, Sinha R, Lameda-Diaz C, Deng L, Nock R, Glass CK, Erbilgin A, Lusis AJ, Witztum JL, Esko JD.  (2014) Reducing macrophage proteoglycan sulfation increases atherosclerosis and obesity through enhanced type I interferon signaling. Cell Metab. 20(5):813-26. PMID: 25440058. PMCID:...

XGlycScan: An Open-source Software For N-linked Glycosite Assignment, Quantification and Quality Assessment of Data from Mass Spectrometry-based Glycoproteomic Analysis.

2014-11-02

Aiyetan P, Zhang B, Zhang Z, Zhang H. (2014) XGlycScan: An Open-source Software For N-linked Glycosite Assignment, Quantification and Quality Assessment of Data from Mass Spectrometry-based Glycoproteomic Analysis. MOJ Proteom Bioinform. 2014;1(1). pii: 00004. PMID: 25346946. PMCID: PMC4207213. Abstract Mass spectrometry based glycoproteomics has become a major...

Global microRNA expression is essential for murine mast cell development in vivo.

2014-10-26

Oh SY, Brandal S, Kapur R, Zhu Z, Takemoto CM. (2014) Global microRNA expression is essential for murine mast cell development in vivo. Exp Hematol. 42(10):919-23. PMID: 25201754. PMCID: PMC4250304. Abstract MicroRNAs (miRNAs) are small, noncoding RNAs that have been shown to play a critical role in...

Irreversible heavy chain transfer to chondroitin

2014-10-17

Lauer ME, Hascall VC, Green DE, DeAngelis PL, Calabro A. (2014) Irreversible heavy chain transfer to chondroitin. J Biol Chem. 289(42):29171-9. PMID: 25135638. PMCID:PMC4200270. Abstract We have recently demonstrated that the transfer of heavy chains (HCs) from inter-α-inhibitor, via the enzyme TSG-6 (tumor necrosis factor-stimulated gene 6),...

Why patients who die of worsening pulmonary arterial hypertension are not on parenteral prostacyclin analog treatment?

2014-10-12

Tonelli AR, Dweik RA. (2014) Why patients who die of worsening pulmonary arterial hypertension are not on parenteral prostacyclin analog treatment? J Heart Lung Transplant. 33(2):221. PMID: 24157181. PMCID: PMC4126496. Link to journal: http://www.jhltonline.org/

Crystal structures of influenza A virus matrix protein M1: variations on a theme.

2014-10-08

Safo MK, Musayev FN, Mosier PD, Zhou Q, Xie H, Desai UR. (2014) Crystal structures of influenza A virus matrix protein M1: variations on a theme. PMID: 25295515. PMCID: PMC4190115. Abstract Matrix protein 1 (M1) of the influenza A virus plays multiple roles in virion assembly and...

The mechanisms of up-regulation of dendritic cell activity by oxidative stress

2014-10-05

Batal I, Azzi J, Mounayar M, Abdoli R, Moore R, Lee JY, Rosetti F, Wang C, Fiorina P, Sackstein R, Ichimura T, Abdi R. (2014) The mechanisms of up-regulation of dendritic cell activity by oxidative stress. J Leukoc Biol. 96(2):283-93. PMID: 24676276. PMCID: PMC4101089. Abstract Whereas DC...

Determinants of versican-V1 proteoglycan processing by the metalloproteinase ADAMTS5.

2014-10-03

Foulcer SJ, Nelson CM, Quintero MV, Kuberan B, Larkin J, Dours-Zimmermann MT, Zimmermann DR, Apte SS. (2014) Determinants of versican-V1 proteoglycan processing by the metalloproteinase ADAMTS5. J Biol Chem. 289(40):27859-73. PMID: 25122765. PMCID: PMC4183820. Abstract Proteolysis of the Glu(441)-Ala(442) bond in the glycosaminoglycan (GAG) β domain of...

Determinants of versican-V1 proteoglycan processing by the metalloproteinase ADAMTS5

2014-10-03

Foulcer SJ, Nelson CM, Quintero MV, Kuberan B, Larkin J, Dours-Zimmermann MT, Zimmermann DR, Apte SS. (2014) Determinants of versican-V1 proteoglycan processing by the metalloproteinase ADAMTS5. J Biol Chem. 289(40):27859-73. PMID: 25122765. PMCID: PMC4183820. Abstract Proteolysis of the Glu(441)-Ala(442) bond in the glycosaminoglycan (GAG) β domain of...

Substantial non-electrostatic forces are needed to induce allosteric disruption of thrombin's active site through exosite 2

2014-09-26

Mehta AY, Desai UR. (2014) Substantial non-electrostatic forces are needed to induce allosteric disruption of thrombin's active site through exosite 2. PMID: 25201728. PMCID: PMC4179500. Abstract Sulfated β-O4 lignin (SbO4L), a non-saccharide glycosaminoglycan mimetic, was recently disclosed as a novel exosite 2-directed thrombin inhibitor with the capability...

Siglec-mediated regulation of immune cell function in disease

2014-09-19

Macauley MS, Crocker PR, Paulson JC. (2014) Siglec-mediated regulation of immune cell function in disease. Nat Rev Immunol. 14(10):653-66. PMID: 25234143. PMCID: PMC4191907. Abstract All mammalian cells display a diverse array of glycan structures that differ from those that are found on microbial pathogens. Siglecs are a...

Glycomic analysis of glycans released from glycoproteins using chemical immobilization and mass spectrometry

2014-09-09

Yang S, Zhang H.(2014) Glycomic analysis of glycans released from glycoproteins using chemical immobilization and mass spectrometry. Curr Protoc Chem Biol. 6(3):191-208. PMID: 25205566. PMCID: PMC4171959. Abstract Protein glycosylation is one of most common protein modifications and is involved in many biological activities. N-linked and O-linked glycosylation...

Sulfated low molecular weight lignins, allosteric inhibitors of coagulation proteinases via the heparin binding site, significantly alter the active site of thrombin and factor xa compared to heparin.

2014-09-06

Henry BL, Desai UR. (2014) Sulfated low molecular weight lignins, allosteric inhibitors of coagulation proteinases via the heparin binding site, significantly alter the active site of thrombin and factor xa compared to heparin. Thromb Res. 134(5):1123-9. PMID: 25242245. PMCID: PMC4253681. Abstract Sulfated low molecular weight lignins (LMWLs)...

Progress in pig-to-non-human primate transplantation models (1998-2013): a comprehensive review of the literature.

2014-09-05

Cooper DK, Satyananda V, Ekser B, van der Windt DJ, Hara H, Ezzelarab MB, Schuurman HJ. (2014) Progress in pig-to-non-human primate transplantation models (1998-2013): a comprehensive review of the literature. Xenotransplantation. 21(5):397-419. PMID: 25176336. PMCID: PMC4176554. Abstract BACKGROUND: The pig-to-non-human primate model is the standard choice for in...

Umbilical cord mesenchymal stem cells suppress host rejection: the role of the glycocalyx

2014-08-22

Coulson-Thomas VJ, Gesteira TF, Hascall V, Kao W. (2014) Umbilical cord mesenchymal stem cells suppress host rejection: the role of the glycocalyx. J Biol Chem. 289(34):23465-81. PMID: 24986866. PMCID: PMC4156039. Abstract Umbilical cord mesenchymal stem cells (UMSCs) have unique immunosuppressive properties enabling them to evade host rejection...

Targeted delivery of mycobacterial antigens to human dendritic cells via Siglec-7 induces robust T cell activation.

2014-08-15

Kawasaki N, Rillahan CD, Cheng TY, Van Rhijn I, Macauley MS, Moody DB, Paulson JC. (2014) Targeted delivery of mycobacterial antigens to human dendritic cells via Siglec-7 induces robust T cell activation. J Immunol. 193(4):1560-6. doi: 10.4049/jimmunol.1303278. PMID: 25000981. PMCID: PMC4119498. Abstract Lipids from mycobacteria can be...

Nutrient Regulation of Transcription, Signaling, and Cell Physiology by O-GlcNAcylation.

2014-08-05

Stéphan Hardivillé and Gerald W. Hart (2014) Nutrient Regulation of Transcription, Signaling, and Cell Physiology by O-GlcNAcylation. Cell Metab. 20:208-213. PMID: 25100062. Abstract The nutrient sensor, O-linked N-acetylglucosamine (O-GlcNAc), cycles on and off nuclear and cytosolic proteins to regulate many cellular processes, including transcription and signaling. Dysregulated...

Regulation of nasal airway homeostasis and inflammation in mice by SHP-1 and Th2/Th1 signaling pathways.

2014-08-04

Cho SH, Oh SY, Lane AP, Lee J, Oh MH, Lee S, Zheng T, Zhu Z. (2014) Regulation of nasal airway homeostasis and inflammation in mice by SHP-1 and Th2/Th1 signaling pathways. PLoS One. 9(8):e103685. doi: 10.1371/journal.pone.0103685. PMID: 25090641. PMCID: PMC4121172. Abstract Allergic rhinitis is a chronic inflammatory disease orchestrated by Th2 lymphocytes. Src...

Metabolomic analysis of breath volatile organic compounds reveals unique breathprints in children with inflammatory bowel disease: a pilot study.

2014-08-04

Patel N, Alkhouri N, Eng K, Cikach F, Mahajan L, Yan C, Grove D, Rome ES, Lopez R, Dweik RA. (2014) Metabolomic analysis of breath volatile organic compounds reveals unique breathprints in children with inflammatory bowel disease: a pilot study. Aliment Pharmacol Ther. 40(5):498-507. PMID:...

Characterization of the specificity of O-GlcNAc reactive antibodies under conditions of starvation and stress.

2014-07-14

Reeves RA, Lee A, Henry R, Zachara NE. (2014) Characterization of the specificity of O-GlcNAc reactive antibodies under conditions of starvation and stress. Anal Biochem. 457:8-18. PMID: 24747005. Abstract The dynamic modification of nuclear, cytoplasmic, and mitochondrial proteins by O-linked β-N-acetyl-D-glucosamine (O-GlcNAc) has been shown to regulate...

Lactosylceramdie :new vis-tas of research.

2014-07-14

Chatterjee S, Mishra S, Suzuki K. Lactosylceramdie :new vis-tas of research. Adv Expt Med 2014 (In Press) Link to journal: http://www.springer.com/series/5584

Molecular mechanisms regulating platelet clearance and thrombopoietin production.

2014-07-14

Grozovsky R, Falet H, Hoffmeister KM. (2014) Molecular mechanisms regulating platelet clearance and thrombopoietin production. Vox Sanguinis, in press. 3339–3347.

The utility of a novel triple marker (combination of TTF1, napsin A, and p40) in the subclassification of non-small cell lung cancer.

2014-07-09

Ao MH, Zhang H, Sakowski L, Sharma R, Illei PB, Gabrielson E, Askin F, Li QK. (2014) The utility of a novel triple marker (combination of TTF1, napsin A, and p40) in the subclassification of non-small cell lung cancer. Hum Pathol. 45(5):926-34. PMID: 24746197. Abstract In lung...

Microbial glycan microarrays define key features of host-microbial interactions.

2014-07-08

Stowell SR, Arthur CM, McBride R, Berger O, Razi N, Heimburg-Molinaro J, Rodrigues LC, Gourdine JP, Noll AJ, von Gunten S, Smith DF, Knirel YA, Paulson JC, Cummings RD. (2014) Microbial glycan microarrays define key features of host-microbial interactions. Nat Chem Biol. 10(6):470-6. PMID: 24814672 Abstract Genomic...

G-CSF Induces Membrane Expression of a Myeloperoxidase Glycovariant that Operates as an E-selectin Ligand on Human Myeloid Cells

2014-07-07

Silvescu C, Sackstein R. (2014) G-CSF Induces Membrane Expression of a Myeloperoxidase Glycovariant that Operates as an E-selectin Ligand on Human Myeloid Cells. Proc Natl Acad Sci U S A. 111(29):10696-701. PMID: 25002508. PMCID: PMC4115493. Abstract The host defense response critically depends on the production of leukocytes...

A computational framework for the automated construction of glycosylation reaction networks.

2014-06-30

Liu G, Neelamegham S. (2014) A computational framework for the automated construction of glycosylation reaction networks. PLoS One. 9(6):e100939. doi: 10.1371/journal.pone.0100939. eCollection 2014. PMID: 24978019. PMCID: PMC4076241. Abstract Glycosylation is among the most common and complex post-translational modifications identified to date. It proceeds through the catalytic action...

Synthetic, Non-saccharide, Glycosaminoglycan Mimetics Selectively Target Colon Cancer Stem Cells.

2014-06-26

Patel NJ, Karuturi R, Al-Horani RA, Baranwal S, Patel J, Desai UR, Patel BB. (2014) Synthetic, Non-saccharide, Glycosaminoglycan Mimetics Selectively Target Colon Cancer Stem Cells. ACS Chem Biol. 2014 Jun 26. PMID: 24968014 [Epub ahead of print] Abstract Selective targeting of cancer stem-like cells (CSCs) is a...

Polyunsaturated fatty acyl-coenzyme As are inhibitors of cholesterol biosynthesis in zebrafish and mice.

2014-06-24

Karanth S, Tran VM, Kuberan B, Schlegel A. (2013) Polyunsaturated fatty acyl-coenzyme As are inhibitors of cholesterol biosynthesis in zebrafish and mice. Dis Model Mech. 6(6):1365-77. PMID: 24057001; PMCID: PMC3820260 Abstract Lipid disorders pose therapeutic challenges. Previously we discovered that mutation of the hepatocyte β-hydroxybutyrate transporter Slc16a6a in zebrafish causes hepatic steatosis during fasting, marked by...

Cross-talk between two essential nutrient-sensitive enzymes: O-GlcNAc transferase (OGT) and AMP-activated protein kinase (AMPK).

2014-06-24

Bullen JW, Balsbaugh JL, Chanda D, Shabanowitz J, Hunt DF, Neumann D, Hart GW. (2014) Cross-talk between two essential nutrient-sensitive enzymes: O-GlcNAc transferase (OGT) and AMP-activated protein kinase (AMPK). J Biol Chem. 289(15):10592-606. PMID: 24563466; PMCID: PMC4036179 Abstract Nutrient-sensitive pathways regulate both O-GlcNAc transferase (OGT) and AMP-activated protein kinase (AMPK), cooperatively connecting metabolic homeostasis to regulation of numerous intracellular...

Identification of Cardiac Myofilament Protein Isoforms Using Multiple Mass Spectrometry Based Approaches.

2014-06-24

Kooij V, Venkatraman V, Kirk JA, Ubaida-Mohien C, Graham DR, Faber MJ, Van Eyk JE. (2014) Identification of Cardiac Myofilament Protein Isoforms Using Multiple Mass Spectrometry Based Approaches. PMID: 24974818. Abstract PURPOSE: The identification of protein isoforms in complex biological samples is challenging. We, therefore, used a mass...

Disubstituted Sialic Acid Ligands Targeting Siglecs CD33 and CD22 Associated with Myeloid Leukaemias and B Cell Lymphomas.

2014-06-24

Rillahan CD, Macauley MS, Schwartz E, He Y, McBride R, Arlian BM, Rangarajan J, Fokin VV, Paulson JC. (2014) Disubstituted Sialic Acid Ligands Targeting Siglecs CD33 and CD22 Associated with Myeloid Leukaemias and B Cell Lymphomas. Chem Sci. 5(6):2398-2406. PMID: 24921038; PMCID: PMC4048721 Abstract The siglec family of sialic acid-binding proteins are endocytic immune cell receptors that are recognized as potential...

The role of platelets in coagulation dysfunction in xenotransplantation, and therapeutic options.

2014-06-14

Iwase H, Ezzelarab MB, Ekser B, Cooper DK. (2014) The role of platelets in coagulation dysfunction in xenotransplantation, and therapeutic options. Xenotransplantation. 2014 May-Jun;21(3):201-20. PMID: 24571124. Abstract Xenotransplantation could resolve the increasing discrepancy between the availability of deceased human donor organs and the demand for transplantation. Most...

Designing Allosteric Inhibitors of Factor XIa. Lessons from the Interactions of Sulfated Pentagalloylglucopyranosides.

2014-06-12

Al-Horani RA, Desai UR. (2014) Designing Allosteric Inhibitors of Factor XIa. Lessons from the Interactions of Sulfated Pentagalloylglucopyranosides. J Med Chem. 57(11):4805-18. PMID: 24844380. Abstract We recently introduced sulfated pentagalloylglucopyranoside (SPGG) as an allosteric inhibitor of factor XIa (FXIa) (Al-Horani et al., J. Med Chem. 2013 ,...

The classical lancefield antigen of group a streptococcus is a virulence determinant with implications for vaccine design.

2014-06-11

van Sorge NM, Cole JN, Kuipers K, Henningham A, Aziz RK, Kasirer-Friede A, Lin L, Berends ET, Davies MR, Dougan G, Zhang F, Dahesh S, Shaw L, Gin J, Cunningham M, Merriman JA, Hütter J, Lepenies B, Rooijakkers SH, Malley R, Walker MJ, Shattil SJ, Schlievert PM, Choudhury B, Nizet V. (2014) The classical lancefield antigen of group a streptococcus is a virulence determinant with implications for vaccine design. Cell...

Inhibition of glycosphingolipid synthesis ameliorates atherosclerosis and arterial stiffness in apolipoprotein e-/- mice and rabbits fed a high-fat and -cholesterol diet.

2014-06-10

Chatterjee S, Bedja D, Mishra S, Amuzie C, Avolio A, Kass DA, Berkowitz D, Renehan M. (2014) Inhibition of glycosphingolipid synthesis ameliorates atherosclerosis and arterial stiffness in apolipoprotein e-/- mice and rabbits fed a high-fat and -cholesterol diet. Circulation. 129(23):2403-13. PMID: 24710030. PMCID: PMC4053506. Abstract BACKGROUND: Glycosphingolipids, integral...

A novel method for pulmonary research: Assessment of bioenergetic function at the air-liquid interface.

2014-06-09

Xu W, Janocha AJ, Leahy RA, Klatte R, Dudzinski D, Mavrakis LA, Comhair SA, Lauer ME, Cotton CU, Erzurum SC. (2014) A novel method for pulmonary research: Assessment of bioenergetic function at the air-liquid interface. Redox Biol. 2:513-9. PMID: 24624341; PMCID: PMC3949089 Abstract Air-liquid interface cell culture is an organotypic model for...

Hyperglycemia diverts dividing osteoblastic precursor cells to an adipogenic pathway and induces synthesis of a hyaluronan matrix that is adhesive for monocytes.

2014-06-09

Wang A, Midura RJ, Vasanji A, Wang AJ, Hascall VC. (2014) Hyperglycemia diverts dividing osteoblastic precursor cells to an adipogenic pathway and induces synthesis of a hyaluronan matrix that is adhesive for monocytes. J Biol Chem. 289(16):11410-20. PMID: 24569987; PMCID: PMC4036277 Abstract Isolated rat bone marrow stromal cells cultured in osteogenic medium in...

Increased soluble CD154 (CD40 ligand) levels in xenograft recipients correlate with the development of de novo anti-pig IgG antibodies

2014-06-09

Ezzelarab MB1, Ekser B, Isse K, Iwase H, Morelli AE, Ayares D, Cooper DK. (2014) Increased soluble CD154 (CD40 ligand) levels in xenograft recipients correlate with the development of de novo anti-pig IgG antibodies. Transplantation. 97(5):502-8. PMID: 244879395 Abstract BACKGROUND: De novo anti-pig antibodies are associated with acute humoral xenograft rejection. We explored the relative efficacy of CD40/CD154-pathway...

Lactosylceramide promotes hypertrophy through ROS generation and activation of ERK1/2 in cardiomyocytes.

2014-06-05

Mishra S, Chatterjee S. (2014) Lactosylceramide promotes hypertrophy through ROS generation and activation of ERK1/2 in cardiomyocytes. Glycobiology. 24(6):518-31. PMID: 24658420. PMCID: PMC4001711 Abstract Hypertrophy is central to several heart diseases; however, not much is known about the role of glycosphingolipids (GSLs) in this phenotype. Since GSLs...

Periostin induces intracellular cross-talk between kinases and hyaluronan in atrioventricular valvulogenesis.

2014-06-02

Ghatak S, Misra S, Norris RA, Moreno-Rodriguez RA, Hoffman S, Levine RA, Hascall VC, Markwald RR. (2014) Periostin induces intracellular cross-talk between kinases and hyaluronan in atrioventricular valvulogenesis. J Biol Chem. 289(12):8545-61. PMID: 24469446; PMCID: PMC3961678 Abstract Periostin (PN), a novel fasciclin-related matricellular protein, has been implicated in cardiac development and postnatal remodeling, but the mechanism remains unknown. We examined...

Factor XI as a target for antithrombotic therapy.

2014-06-02

Bane CE Jr, Gailani D. (2014) Factor XI as a target for antithrombotic therapy. Drug Discov Today. 2014 Jun 2. pii: S1359-6446(14)00225-6. PMID: 24886766. Abstract Anticoagulants currently used in clinical practice to treat thromboembolic disorders are effective but increase the risk of severe bleeding because they target...

PDE5 inhibitor efficacy is estrogen dependent in female heart disease.

2014-06-02

Sasaki H, Nagayama T, Blanton RM, Seo K, Zhang M, Zhu G, Lee DI, Bedja D, Hsu S, Tsukamoto O, Takashima S, Kitakaze M, Mendelsohn ME, Karas RH, Kass DA, Takimoto E. (2014) PDE5 inhibitor efficacy is estrogen dependent in female heart disease. J Clin...

Congenital disorder of fucosylation type 2c (LADII) presenting with short stature and developmental delay with minimal adhesion defect.

2014-06-01

Dauber A, Ercan A, Lee J, James P, Jacobs PP, Ashline DJ, Wang SR, Miller T, Hirschhorn JN, Nigrovic PA, Sackstein R. (2014) Congenital disorder of fucosylation type 2c (LADII) presenting with short stature and developmental delay with minimal adhesion defect. Hum Mol Genet. 23(11):2880-7....

Discovery methodology for the development of direct factor VIIa inhibitors.

2014-05-31

Henry BL, Desai UR. (2014) Discovery methodology for the development of direct factor VIIa inhibitors. Expert Opin Drug Discov. 2014 May 31:1-14. PMID: 24882057. Abstract Introduction: Heparin and warfarin have historically been the only antithrombotics available. Recently, however, newer anticoagulants have been developed. Factor VIIa (fVIIa) inhibitors...

Rapid evolution of binding specificities and expression patterns of inhibitory CD33-related Siglecs in primates.

2014-05-31

Padler-Karavani V, Hurtado-Ziola N, Chang YC, Sonnenburg JL, Ronaghy A, Yu H, Verhagen A, Nizet V, Chen X, Varki N, Varki A, Angata T. (2014) Rapid evolution of binding specificities and expression patterns of inhibitory CD33-related Siglecs in primates. FASEB J. 28(3):1280-93. PMID: 24308974; PMCID: PMC3929681   Abstract Siglecs are sialic acid-binding Ig-like lectins that recognize sialoglycans via amino-terminal V-set domains. CD33-related Siglecs (CD33rSiglecs) on...

Heparin prevents intracellular hyaluronan synthesis and autophagy responses in hyperglycemic dividing mesangial cells and activates synthesis of an extensive extracellular monocyte-adhesive hyaluronan matrix after completing cell division.

2014-05-30

Wang A1, Ren J, Wang CP, Hascall VC. (2014) Heparin prevents intracellular hyaluronan synthesis and autophagy responses in hyperglycemic dividing mesangial cells and activates synthesis of an extensive extracellular monocyte-adhesive hyaluronan matrix after completing cell division. J Biol Chem. 289(13):9418-29. PMID: 24482224; PMCID: PMC3979390   Abstract Growth-arrested rat mesangial cells (RMCs) at a G0/G1 interphase stimulated to...

Overexpression of c-Met and CD44v6 receptors contributes to autocrine TGF-β1 signaling in interstitial lung disease.

2014-05-30

Ghatak S, Bogatkevich GS, Atnelishvili I, Akter T, Feghali-Bostwick C, Hoffman S, Fresco VM, Fuchs JC, Visconti RP, Markwald RR, Padhye SB, Silver RM,Hascall VC, Misra S. (2014) Overexpression of c-Met and CD44v6 receptors contributes to autocrine TGF-β1 signaling in interstitial lung disease. J Biol Chem. 289(11):7856-72. PMID: 24324260; PMCID: PMC3953297   Abstract The hepatocyte growth factor (HGF) and the HGF receptor Met pathway are important...

Platelets support extracellular sialylation by supplying the sugar donor substrate.

2014-05-30

Lee MM1, Nasirikenari M, Manhardt CT, Ashline DJ, Hanneman AJ, Reinhold VN, Lau JT. (2014) Platelets support extracellular sialylation by supplying the sugar donor substrate. J Biol Chem. 289(13):8742-8. PMID: 24550397; PMCID: PMC3979374   Abstract Sizable pools of freely circulating glycosyltransferases are in blood, but understanding their physiologic contributions has been hampered because functional sources of sugar donor substrates...

A serotonin-induced N-glycan switch regulates platelet aggregation.

2014-05-30

Mercado CP, Quintero MV, Li Y, Singh P, Byrd AK, Talabnin K, Ishihara M, Azadi P, Rusch NJ, Kuberan B, Maroteaux L, Kilic F. (2013) A serotonin-induced N-glycan switch regulates platelet aggregation. Sci Rep. 3:2795. PMID: 24077408; PMCID: PMC3786303 Abstract Serotonin (5-HT) is a multifunctional signaling molecule that plays different roles in a concentration-dependent manner. We demonstrated that elevated levels of plasma 5-HT accelerate...

Hyaluronan digestion controls DC migration from the skin.

2014-05-30

Muto J, Morioka Y, Yamasaki K, Kim M, Garcia A, Carlin AF, Varki A, Gallo RL. (2014) Hyaluronan digestion controls DC migration from the skin. J Clin Invest. 124(3):1309-19. PMID: 24487587; PMCID: PMC3934161 Abstract The breakdown and release of hyaluronan (HA) from the extracellular matrix has been hypothesized to act as an endogenous signal of injury. To test this hypothesis,...

Expansion of the neonatal platelet mass is achieved via an extension of platelet lifespan.

2014-05-29

Liu ZJ, Hoffmeister KM, Hu Z, Mager DE, Ait-Oudhia S, Debrincat MA, Pleines I, Josefsson EC, Kile BT, Italiano J Jr, Ramsey H, Grozovsky R, Veng-Pedersen P, Chavda C, Sola-Visner M. (2014) Expansion of the neonatal platelet mass is achieved via an extension of platelet...

Sulfated Caffeic Acid Dehydropolymer Attenuates Elastase and Cigarette Smoke Extract-induced Emphysema in Rats: Sustained Activity and a Need of Pulmonary Delivery.

2014-05-16

Saluja B, Li H, Desai UR, Voelkel NF, Sakagami M. (2014) Sulfated Caffeic Acid Dehydropolymer Attenuates Elastase and Cigarette Smoke Extract-induced Emphysema in Rats: Sustained Activity and a Need of Pulmonary Delivery. Lung. 2014 May 16. (In Press) PMID: 24831783. Abstract BACKGROUND: Although emphysema destroys alveolar structures progressively...

Sublingual microcirculation in pulmonary arterial hypertension

2014-05-14

Dababneh L, Cikach F, Alkukhun L, Dweik RA, Tonelli AR.(2014) Sublingual microcirculation in pulmonary arterial hypertension. Ann Am Thorac Soc. 11(4):504-12. PMID: 24601682. PMCID: PMC4225801. Abstract RATIONALE:  Pulmonary arterial hypertension (PAH) is a pulmonary vasculopathy that leads to failure of the right ventricle and premature death. OBJECTIVES:  To...

Siglec-5 and Siglec-14 are polymorphic paired receptors that modulate neutrophil and amnion signaling responses to group B Streptococcus.

2014-05-05

Ali SR, Fong JJ, Carlin AF, Busch TD, Linden R, Angata T, Areschoug T, Parast M, Varki N, Murray J, Nizet V, Varki A. (2014) Siglec-5 and Siglec-14 are polymorphic paired receptors that modulate neutrophil and amnion signaling responses to group B Streptococcus. J Exp...

Role of macrophage sialoadhesin in host defense against the sialylated pathogen group B Streptococcus.

2014-05-03

Chang YC, Olson J, Louie A, Crocker PR, Varki A, Nizet V. (2014) Role of macrophage sialoadhesin in host defense against the sialylated pathogen group B Streptococcus. J Mol Med (Berl). [Epub ahead of print] PMID: 24788876. Abstract Several bacterial pathogens decorate their surfaces with sialic acid...

Presence and outcomes of kidney disease in patients with pulmonary hypertension.

2014-05-02

Navaneethan SD, Wehbe E, Heresi GA, Gaur V, Minai OA, Arrigain S, Nally JV Jr, Schold JD, Rahman M, Dweik RA. (2014) Presence and outcomes of kidney disease in patients with pulmonary hypertension. Clin J Am Soc Nephrol. 9(5):855-63. PMID: 24578332. PMCID: PMC4011456. Abstract BACKGROUND AND OBJECTIVES: Pulmonary...

Mechanisms of Siglec-F-induced eosinophil apoptosis: a role for caspases but not for SHP-1, Src kinases, NADPH oxidase or reactive oxygen.

2014-04-28

Mao H, Kano G, Hudson SA, Brummet M, Zimmermann N, Zhu Z, Bochner BS. (2013) Mechanisms of Siglec-F-induced eosinophil apoptosis: a role for caspases but not for SHP-1, Src kinases, NADPH oxidase or reactive oxygen. PLoS One. 8(6):e68143. PMID: 23840825; PMCID: PMC3695997 Abstract BACKGROUND: Siglec-F and Siglec-8 are functional paralog proapoptotic cell surface receptors expressed on mouse and human eosinophils,...

In-gel β-elimination and aqueous-organic partition for improved O- and sulfoglycomics.

2014-04-28

Kumagai T, Katoh T, Nix DB, Tiemeyer M, Aoki K. (2013) In-gel β-elimination and aqueous-organic partition for improved O- and sulfoglycomics. Anal Chem. 85(18):8692-9. PMID: 23937624 Abstract Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique for protein separation, and in-gel tryptic digestion of resolved protein bands has enhanced the resolution of protoeomic analysis....

Breath analysis in pulmonary arterial hypertension.

2014-04-28

Cikach FS Jr, Tonelli AR, Barnes J, Paschke K, Newman J, Grove D, Dababneh L, Wang S, Dweik RA. (2014) Breath analysis in pulmonary arterial hypertension. Chest. 145(3):551-8. PMID: 24091389; PMCID: PMC3941248   Abstract BACKGROUND: Pulmonary arterial hypertension (PAH) is a progressive and devastating condition characterized by vascular cell proliferation and is associated with several metabolic derangements. We hypothesized that metabolic derangements in...

M2Lite: An Open-source, Light-weight, Pluggable and Fast Proteome Discoverer MSF to mzIdentML Tool.

2014-04-28

Aiyetan P, Zhang B, Chen L, Zhang Z, Zhang H.(2014) M2Lite: An Open-source, Light-weight, Pluggable and Fast Proteome Discoverer MSF to mzIdentML Tool. J Bioinform. 1(2):40-49. PMID: 25346941. PMCID: PMC4206089. Abstract Proteome Discoverer is one of many tools used for protein database search and peptide to spectrum...

Allosteric inhibition of human factor XIa: discovery of monosulfated benzofurans as a class of promising inhibitors.

2014-04-24

Argade MD, Mehta AY, Sarkar A, Desai UR. (2014) Allosteric inhibition of human factor XIa: discovery of monosulfated benzofurans as a class of promising inhibitors. J Med Chem. 57(8):3559-69. PMID: 24666186. Abstract Factor XIa (fXIa) is being recognized as a prime target for developing safer anticoagulants. To...

A new Adamts9 conditional mouse allele identifies its non-redundant role in interdigital web regression.

2014-04-20

Dubail J, Aramaki-Hattori N, Bader HL, Nelson CM, Katebi N, Matuska B, Olsen BR, Apte SS. (2014) A new Adamts9 conditional mouse allele identifies its non-redundant role in interdigital web regression. Genesis. 2014 Apr 20. doi: 10.1002/dvg.22784. PMID: 24753090. Abstract ADAMTS9 is the most conserved member of...

Hyperglycemia diverts dividing osteoblastic precursor cells to an adipogenic pathway and induces synthesis of a hyaluronan matrix that is adhesive for monocytes.

2014-04-18

Wang A, Midura RJ, Vasanji A, Wang AJ, Hascall VC. (2014) Hyperglycemia diverts dividing osteoblastic precursor cells to an adipogenic pathway and induces synthesis of a hyaluronan matrix that is adhesive for monocytes. J Biol Chem. 289(16):11410-20. PMID: 24569987. Abstract: Isolated rat bone marrow stromal cells...

Proline substitution of dimer interface β-strand residues as a strategy for the design of functional monomeric proteins.

2014-04-14

Joseph PR, Poluri KM, Gangavarapu P, Rajagopalan L, Raghuwanshi S, Richardson RM, Garofalo RP, Rajarathnam K. (2013) Proline substitution of dimer interface β-strand residues as a strategy for the design of functional monomeric proteins. Biophys J. 17;105(6):1491-501. PMID: 24048001; PMCID: PMC3785864 Abstract Proteins that exist in monomer-dimer equilibrium can be found in all organisms ranging from bacteria to humans; this...

No monkeying around: clonal tracking of stem cells and progenitors in the macaque.

2014-04-14

Dykstra B, Bystrykh LV. (2014) No monkeying around: clonal tracking of stem cells and progenitors in the macaque. Cell Stem Cell. 14(4):419-20. PMID: 24702990 Abstract Clonal tracking of hematopoietic stem and progenitor cells (HSPCs) has proven valuable for studying their behavior in murine recipients. Now in Cell Stem Cell, Kim et al. (2014) and...

Dynamic O-GlcNAcylation and its roles in the cellular stress response and homeostasis.

2014-04-14

Groves JA1, Lee A, Yildirir G, Zachara NE. (2013) Dynamic O-GlcNAcylation and its roles in the cellular stress response and homeostasis. Cell Stress Chaperones. 18(5):535-58. PMID: 23620203; PMCID: PMC3745259 Abstract O-linked N-acetyl-β-D-glucosamine (O-GlcNAc) is a ubiquitous and dynamic post-translational modification known to modify over 3,000 nuclear, cytoplasmic, and mitochondrial eukaryotic proteins. Addition of O-GlcNAc to proteins...

Nutrient regulation of immunity: O-GlcNAcylation regulates stimulus-specific NF-κB-dependent transcription.

2014-04-14

Hart GW. (2013) Nutrient regulation of immunity: O-GlcNAcylation regulates stimulus-specific NF-κB-dependent transcription. Sci Signal. 6(290):pe26. PMID: 23982203   Abstract Nutrients regulate gene transcription by the dynamic cycling of O-linked N-acetylglucosamine (O-GlcNAc) on proteins that constitute the transcriptional machinery. A study shows that O-GlcNAcylation of the nuclear factor κB (NF-κB) subunit c-Rel...

Glycoproteomic and glycomic databases.

2014-04-13

Baycin Hizal D, Wolozny D, Colao J, Jacobson E, Tian Y, Krag SS, Betenbaugh MJ, Zhang H. (2014) Glycoproteomic and glycomic databases. Clin Proteomics. 11(1):15. PMID: 24725457. PMCID: PMC3996109. Abstract Protein glycosylation serves critical roles in the cellular and biological processes of many organisms. Aberrant glycosylation has...

Targeting the GPIbα binding site of thrombin to simultaneously induce dual anticoagulant and antiplatelet effects.

2014-04-10

Mehta AY, Thakkar JN, Mohammed BM, Martin EJ, Brophy DF, Kishimoto T, Desai UR. (2014) Targeting the GPIbα binding site of thrombin to simultaneously induce dual anticoagulant and antiplatelet effects. J Med Chem. 57(7):3030-9. PMID: 24635452. Abstract Exosite 2 of human thrombin contributes to two opposing pathways,...

Distribution of non-gal antigens in pig cornea: relevance to corneal xenotransplantation.

2014-04-09

Cohen D, Miyagawa Y, Mehra R, Lee W, Isse K, Long C, Ayares DL, Cooper DK, Hara H. (2014) Distribution of non-gal antigens in pig cornea: relevance to corneal xenotransplantation. Cornea. 33:390-7. PMID: 24488129. Abstract PURPOSE: The aim of this study was to investigate the distribution of antigens...

Heparan sulfate 3-O-sulfation: A rare modification in search of a function.

2014-04-09

Thacker BE, Xu D, Lawrence R, Esko JD. (2014) Heparan sulfate 3-O-sulfation: A rare modification in search of a function. Matrix Biol.35:60-72. PMID: 24361527. PMCID: PMC4039620. Abstract Many protein ligands bind to heparan sulfate, which results in their presentation, protection, oligomerization or conformational activation. Binding depends on...

The dynamic metabolism of hyaluronan regulates the cytosolic concentration of UDP-GlcNAc.

2014-04-08

Hascall VC, Wang A, Tammi M, Oikari S, Tammi R, Passi A, Vigetti D, Hanson RW, Hart GW. (2014) The dynamic metabolism of hyaluronan regulates the cytosolic concentration of UDP-GlcNAc. Matrix Biol. 35:14-7. PMID: 24486448. PMCID: PMC4039572. Abstract Hyaluronan, a macromolecular glycosaminoglycan, is normally synthesized by hyaluronan...

The dynamic metabolism of hyaluronan regulates the cytosolic concentration of UDP-GlcNAc.

2014-04-05

Hascall VC, Wang A, Tammi M, Oikari S, Tammi R, Passi A, Vigetti D, Hanson RW, Hart GW. (2014) The dynamic metabolism of hyaluronan regulates the cytosolic concentration of UDP-GlcNAc. Matrix Biol. 35:14-7. PMID: 24486448. PMCID: PMC4039572. Abstract Hyaluronan, a macromolecular glycosaminoglycan, is normally synthesized by hyaluronan...

Chemogenesis of an antiangiogenic glycosaminoglycan.

2014-04-04

Raman K, Arungundram S, Kuberan B. (2014) Chemogenesis of an antiangiogenic glycosaminoglycan. ACS Med Chem Lett. 5(6):644-6. PMID: 24944736. PMCID: PMC4060934. Abstract In this letter we report a facile chemical conversion of heparin, a potent anticoagulant with minimal antiangiogenic activity, into an effective antiangiogenic glycosaminoglycan through optimized...

The multiple, complex roles of versican and its proteolytic turnover by ADAMTS proteases during embryogenesis.

2014-04-02

Nandadasa S, Foulcer S, Apte SS. (2014) The multiple, complex roles of versican and its proteolytic turnover by ADAMTS proteases during embryogenesis. Matrix Biol. 35:34-41. PMID: 2444473. Abstract Embryonic development is an exceptionally dynamic process, requiring a provisional extracellular matrix that is amenable to rapid remodeling, and...

Solution structure of CXCL5--a novel chemokine and adipokine implicated in inflammation and obesity.

2014-04-02

Sepuru KM, Poluri KM, Rajarathnam K. (2014) Solution structure of CXCL5--a novel chemokine and adipokine implicated in inflammation and obesity. PLoS One. 9(4):e93228. PMID: 24695525. PMCID: PMC3973705. Abstract The chemokine CXCL5 is selectively expressed in highly specialized cells such as epithelial type II cells in the lung...

CCR5 inhibition prevents cardiac dysfunction in the SIV/macaque model of HIV.

2014-04-02

Kelly KM, Tocchetti CG, Lyashkov A, Tarwater PM, Bedja D, Graham DR, Beck SE, Metcalf Pate KA, Queen SE, Adams RJ, Paolocci N, Mankowski JL. (2014) CCR5 inhibition prevents cardiac dysfunction in the SIV/macaque model of HIV. J Am Heart Assoc.3(2):e000874. PMID: 24695652. Abstract BACKGROUND: Diastolic dysfunction is...

Structural documentation of glycan epitopes: sequential mass spectrometry and spectral matching

2014-03-25

Ashline DJ, Hanneman AJ, Zhang H, Reinhold VN. (2014) Structural documentation of glycan epitopes: sequential mass spectrometry and spectral matching. J Am Soc Mass Spectrom. 25(3):444-53. PMID: 24385394. PMCID: PMC3950938. Abstract Documenting mass spectral data is a fundamental aspect of accepted protocols. In this report, we contrast...

Recent Advances on Plasmin Inhibitors for the Treatment of Fibrinolysis-Related Disorders.

2014-03-21

Al-Horani RA, Desai UR. (2014) Recent Advances on Plasmin Inhibitors for the Treatment of Fibrinolysis-Related Disorders. Med Res Rev. 2014 Mar 21. doi: 10.1002/med.21315. (In Press) PMID: 24659483. Abstract Growing evidence suggests that plasmin is involved in a number of physiological processes in addition to its key...

The Breathprints in Patients With Liver Disease Identify Novel Breath Biomarkers in Alcoholic Hepatitis.

2014-03-14

Hanouneh IA, Zein NN, Cikach F, Dababneh L, Grove D, Alkhouri N, Lopez R, Dweik RA. (2014) The Breathprints in Patients With Liver Disease Identify Novel Breath Biomarkers in Alcoholic Hepatitis. Clin Gastroenterol Hepatol. 12(3):516-23. PMID: 24036050. PMCID: PMC3971429. Abstract BACKGROUND & AIMS: Selected-ion flow-tube mass spectrometry can...

Hyaluronan, a crucial regulator of inflammation.

2014-03-11

Petrey AC, de la Motte CA. (2014) Hyaluronan, a crucial regulator of inflammation. Front Immunol.5:101. PMID: 24653726. PMCID: PMC3949149. Abstract Hyaluronan (HA), a major component of the extracellular matrix (ECM), plays a key role in regulating inflammation. Inflammation is associated with accumulation and turnover of HA polymers...

Modification of hyaluronan by heavy chains of inter-α-inhibitor in idiopathic pulmonary arterial hypertension.

2014-03-08

Lauer ME, Aytekin M, Comhair SA, Loftis J, Tian L, Farver CF, Hascall VC, Dweik RA. (2014) Modification of hyaluronan by heavy chains of inter-α-inhibitor in idiopathic pulmonary arterial hypertension. J Biol Chem. 289(10):6791-8. PMID: 24403074. PMCID: PMC3945340. Abstract We previously reported an altered hyaluronan (HA) metabolism...

Remodeling of marrow hematopoietic stem and progenitor cells by non-self ST6Gal-1 sialyltransferase.

2014-03-07

Nasirikenari M, Veillon L, Collins CC, Azadi P, Lau JT. (2014) Remodeling of marrow hematopoietic stem and progenitor cells by non-self ST6Gal-1 sialyltransferase. J Biol Chem. 289(10):7178-89. PMID: 24425878. PMCID: PMC3945377. Abstract Glycans occupy the critical cell surface interface between hematopoietic cells and their marrow niches. Typically,...

Demystifying heparan sulfate-protein interactions.

2014-03-06

Xu D, Esko JD. (2014) Demystifying heparan sulfate-protein interactions. Annu Rev Biochem. 83:129-57. PMID: 24606135. Abstract Numerous proteins, including cytokines and chemokines, enzymes and enzyme inhibitors, extracellular matrix proteins, and membrane receptors, bind heparin. Although they are traditionally classified as heparin-binding proteins, under normal physiological conditions these...

O-GlcNAc profiling: from proteins to proteomes.

2014-03-05

Ma J, Hart GW. (2014) O-GlcNAc profiling: from proteins to proteomes. Clin Proteomics. 11(1):8. PMID: 24593906. PMCID: PMC4015695. Abstract O-linked β-D-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) onto serine and threonine residues of proteins is an important post-translational modification (PTM), which is involved in many crucial biological processes including transcription,...

Long-term continuous positive airway pressure therapy normalizes high exhaled nitric oxide levels in obstructive sleep apnea.

2014-03-03

Chua AP, Aboussouan LS, Minai OA, Paschke K, Laskowski D, Dweik RA. (2013) Long-term continuous positive airway pressure therapy normalizes high exhaled nitric oxide levels in obstructive sleep apnea. J Clin Sleep Med. 9(6):529-35. PMID: 23772184; PMCID: PMC3659371   Abstract STUDY OBJECTIVES: Upper airway inflammation and oxidative stress have been implicated in the pathogenesis of obstructive sleep...

Corneal blindness and xenotransplantation.

2014-03-01

Lamm V, Hara H, Mammen A, Dhaliwal D, Cooper DK. (2014) Corneal blindness and xenotransplantation. Xenotransplantation. 21(2):99-114. PMID: 25268248. PMCID: PMC4181387. Abstract Approximately 39 million people are blind worldwide, with an estimated 285 million visually impaired. The developing world shoulders 90% of the world's blindness, with 80% of...

Hyperactive adverse mechanical stress responses in dystrophic heart are coupled to transient receptor potential canonical 6 and blocked by cGMP-protein kinase G modulation.

2014-02-28

Seo K, Rainer PP, Lee DI, Hao S, Bedja D, Birnbaumer L, Cingolani OH, Kass DA. (2014) Hyperactive adverse mechanical stress responses in dystrophic heart are coupled to transient receptor potential canonical 6 and blocked by cGMP-protein kinase G modulation. Circ Res. 114(5):823-32. PMID: 24449818....

Kidney xenotransplantation.

2014-02-22

Cowan PJ, Cooper DK, d'Apice AJ. (2014) Kidney xenotransplantation. Kidney Int. 85(2):265-75.  PMID: 24088952 PMCID: PMC3946635. Abstract Xenotransplantation using pigs as donors offers the possibility of eliminating the chronic shortage of donor kidneys, but there are several obstacles to be overcome before this goal can be achieved. Preclinical studies have shown that, while...

Synthesis of fluorophore-tagged xylosides that prime glycosaminoglycan chains.

2014-02-19

Tran VM, Kuberan B. (2014) Synthesis of fluorophore-tagged xylosides that prime glycosaminoglycan chains. Bioconjug Chem. 25(2):262-8. PMID: 24499349. PMCID: PMC3983138. Abstract Biosynthesis and functions of glycosaminoglycan (GAG) chains are complex and remain elusive. To better understand the factors that regulate the biosynthesis and functions, fluorophore-tagged xylosides...

Time-resolved analysis of the matrix metalloproteinase 10 substrate degradome.

2014-02-13

Schlage P, Egli FE, Nanni P, Wang LW, Kizhakkedathu JN, Apte SS, auf dem Keller U. (2014) Time-resolved analysis of the matrix metalloproteinase 10 substrate degradome. Mol Cell Proteomics. 13:580-93. PMID: 24281761. PMCID: PMC3916655. Abstract Proteolysis is an irreversible post-translational modification that affects intra- and intercellular communication...

The potential role of genetically-modified pig mesenchymal stromal cells in xenotransplantation.

2014-02-06

Li J, Ezzelarab MB, Ayares D, Cooper DK. (2014) The potential role of genetically-modified pig mesenchymal stromal cells in xenotransplantation. Stem Cell Rev. 10(1):79-85. PMID: 24142483. PMCID: PMC3946698. Abstract Mesenchymal stromal cells (MSCs) are known to have regenerative, anti-inflammatory, and immunodulatory effects. There are extensive indications that...

Characterization of human CD39+ Th17 cells with suppressor activity and modulation in inflammatory bowel disease.

2014-02-05

Longhi MS, Moss A, Bai A, Wu Y, Huang H, Cheifetz A, Quintana FJ, Robson SC. (2014) Characterization of human CD39+ Th17 cells with suppressor activity and modulation in inflammatory bowel disease. PLoS One. 9(2):e87956. PMID: 24505337. PMCID: PMC3914873 Abstract Induced regulatory T-cells (iT-reg) and T helper...

Glycoproteomics using fluid-based specimens in the discovery of lung cancer protein biomarkers: promise and challenge.

2014-02-03

Li QK, Gabrielson E, Askin F, Chan DW, Zhang H. (2013) Glycoproteomics using fluid-based specimens in the discovery of lung cancer protein biomarkers: promise and challenge. Proteomics Clin Appl. 7(1-2):55-69. PMID: 23112109 Abstract Lung cancer is the leading cancer in the United States and worldwide. In spite of the rapid progression in personalized treatments, the overall...

Synthesis and assessment of glycosaminoglycan priming activity of cluster-xylosides for potential use as proteoglycan mimetics.

2014-02-03

Tran VM, Nguyen TK, Sorna V, Loganathan D, Kuberan B. (2013) Synthesis and assessment of glycosaminoglycan priming activity of cluster-xylosides for potential use as proteoglycan mimetics. ACS Chem Biol. 8(5):949-57. PMID: 23402705 Abstract One of the distinct structural features of many proteoglycans (PGs) is the presence of two or more glycosaminoglycan (GAG) side chains covalently linked...

Dimerized glycosaminoglycan chains increase FGF signaling during zebrafish development.

2014-02-03

Nguyen TK, Tran VM, Sorna V, Eriksson I, Kojima A, Koketsu M, Loganathan D, Kjellén L, Dorsky RI, Chien CB, Kuberan B. (2013) Dimerized glycosaminoglycan chains increase FGF signaling during zebrafish development. ACS Chem Biol. 8(5):939-48. PMID: 23614643 Abstract Proteoglycans (PGs) modulate numerous signaling pathways during development through binding of their glycosaminoglycan (GAG) side chains to various signaling molecules, including fibroblast growth...

Inhibition of protein carbamylation in urea solution using ammonium-containing buffers.

2014-02-01

Sun S, Zhou JY, Yang W, Zhang H. (2014) Inhibition of protein carbamylation in urea solution using ammonium-containing buffers. Anal Biochem. 446:76-81. PMID: 24161613. PMCID: PMC4072244. Abstract Urea solution is one of the most commonly employed protein denaturants for protease digestion in proteomic studies. However, it has...

Analysis of breath volatile organic compounds as a noninvasive tool to diagnose nonalcoholic fatty liver disease in children.

2014-01-23

Alkhouri N, Cikach F, Eng K, Moses J, Patel N, Yan C, Hanouneh I, Grove D, Lopez R, Dweik R. (2014) Analysis of breath volatile organic compounds as a noninvasive tool to diagnose nonalcoholic fatty liver disease in children. Eur J Gastroenterol Hepatol. 26(1):82-7. PMID:...

Solid-phase glycan isolation for glycomics analysis.

2014-01-23

Yang S, Zhang H. (2012) Solid-phase glycan isolation for glycomics analysis. Proteomics Clin Appl. 6(11-12):596-608. PMID: 23090885; PMCID: PMC3674833. Abstract Glycosylation is one of the most significant protein PTMs. The biological activities of proteins are dramatically changed by the glycans associated with them. Thus, structural analysis of the glycans of glycoproteins in complex...

Wiskott-Aldrich syndrome protein (WASp) controls the delivery of platelet transforming growth factor-β1.

2014-01-22

Kim H, Falet H, Hoffmeister KM, Hartwig JH. (2013) Wiskott-Aldrich syndrome protein (WASp) controls the delivery of platelet transforming growth factor-β1. J Biol Chem. 288(48):34352-63. PMID: 24133214; PMCID: PMC3843050 Abstract Platelets are immunologically competent cells containing cytokines such as TGF-β1 that regulate cell-mediated immunity. However, the mechanisms underlying cytokine secretion from platelets are undefined. The...

An update on recent patents on thrombin inhibitors (2010 - 2013).

2014-01-20

Mehta AY, Jin Y, Desai UR. (2014) An update on recent patents on thrombin inhibitors (2010 - 2013). Expert Opin Ther Pat. 24(1):47-67. PMID: 24099091. Abstract INTRODUCTION: Thromboembolic incidences have increased nearly 33% in the past decade and directly affect nearly 0.5% of the population. Heparin, warfarin and...

Synthesis of Biologically Active N- and O-Linked Glycans with Multisialylated Poly-N-acetyllactosamine Extensions Using P. damsela α2-6 Sialyltransferase.

2014-01-16

Nycholat CM, Peng W, McBride R, Antonopoulos A, de Vries RP, Polonskaya Z, Finn MG, Dell A, Haslam SM, Paulson JC. Synthesis of Biologically Active N- and O-Linked Glycans with Multisialylated Poly-N-acetyllactosamine Extensions Using P. damsela α2-6 Sialyltransferase. J Am Chem Soc. 135(49):18280-3. PMID: 24256304 PMCID: PMC3901641.   Abstract Sialosides on N- and O-linked glycoproteins play a fundamental role in many biological...

Dynamic conformational switching in the chemokine ligand is essential for G-protein-coupled receptor activation.

2014-01-16

Joseph PR, Sawant KV, Isley A, Pedroza M, Garofalo RP, Richardson RM, Rajarathnam K. (2013) Dynamic conformational switching in the chemokine ligand is essential for G-protein-coupled receptor activation. Biochem J. 456(2):241-51. PMID: 24032673   Abstract Chemokines mediate diverse functions from organogenesis to mobilizing leucocytes, and are unusual agonists for class-A GPCRs (G-protein-coupled receptors) because of their large size and...

A nanosensor for ultrasensitive detection of oversulfated chondroitin sulfate contaminant in heparin.

2014-01-15

Kalita M, Balivada S, Swarup VP, Mencio C, Raman K, Desai UR, Troyer D, Kuberan B. (2014) A nanosensor for ultrasensitive detection of oversulfated chondroitin sulfate contaminant in heparin. J Am Chem Soc. 136(2):554-7. PMID: 24127748. Abstract Heparin has been extensively used as an anticoagulant for the...

Group B Streptococcus engages an inhibitory Siglec through sialic acid mimicry to blunt innate immune and inflammatory responses in vivo.

2014-01-12

Chang YC, Olson J, Beasley FC, Tung C, Zhang J, Crocker PR, Varki A, Nizet V. (2014) Group B Streptococcus engages an inhibitory Siglec through sialic acid mimicry to blunt innate immune and inflammatory responses in vivo. PLoS Pathog. 10(1):e1003846. PMID: 24391502. PMCID: PMC3879367 Abstract Group B...

Isothermal titration calorimetry of membrane proteins - progress and challenges.

2014-01-09

Rajarathnam K, Rösgen J. (2014) Isothermal titration calorimetry of membrane proteins - progress and challenges. Biochim Biophys Acta. 1838(1 Pt A):69-77. PMID: 23747362. Abstract Integral membrane proteins, including G protein-coupled receptors (GPCR) and ion channels, mediate diverse biological functions that are crucial to all aspects of life....

Regulation of human platelet aggregation by genetically modified pig endothelial cells and thrombin inhibition.

2014-01-06

Iwase H, Ekser B, Hara H, Phelps C, Ayares D, Cooper DK, Ezzelarab MB. (2013) Regulation of human platelet aggregation by genetically modified pig endothelial cells and thrombin inhibition. Xenotransplantation. [Epub]. PMID: 24188473. Abstract BACKGROUND: Coagulation disorders remain barriers to successful pig-to-primate organ xenotransplantation. In vitro, we investigated the impact of pig genetic modifications on human platelet aggregation in...

Molecular basis of glycosaminoglycan heparin binding to the chemokine CXCL1 dimer.

2014-01-06

Poluri KM, Joseph PR, Sawant KV, Rajarathnam K. (2013) Molecular basis of glycosaminoglycan heparin binding to the chemokine CXCL1 dimer. J Biol Chem. 288(35):25143-53. PMID: 23864653; PMCID: PMC3757178. Abstract Glycosaminoglycan (GAG)-bound and soluble chemokine gradients in the vasculature and extracellular matrix mediate neutrophil recruitment to the site of microbial infection and sterile injury in the host...

Sulfation patterns determine cellular internalization of heparin-like polysaccharides.

2014-01-06

Raman K, Mencio C, Desai UR, Kuberan B. (2013) Sulfation patterns determine cellular internalization of heparin-like polysaccharides. Mol Pharm. 10(4):1442-9. PMID: 23398560; PMCID: PMC3685167 Abstract Heparin is a highly sulfated polysaccharide that serves biologically relevant roles as an anticoagulant and anticancer agent. While it is well-known that modification of heparin's sulfation pattern can drastically influence its...

Ischemic preconditioning increases myocardial O-GlcNAc glycosylation.

2014-01-06

Vibjerg Jensen R, Johnsen J, Buus Kristiansen S, Zachara NE, Bøtker HE. Ischemic preconditioning increases myocardial O-GlcNAc glycosylation. (2013) Scand Cardiovasc J. 47(3):168-74. PMID: 23301939.   Abstract OBJECTIVES: Through the hexosamine biosynthetic pathway (HBP) proteins are modified by O-linked-β-N-acetylglucosamine (O-GlcNAc), which acts as a stress sensor. Augmentation of O-GlcNAc confers cardioprotection against ischemia- reperfusion injury, but its role...

Use of a glycolipid inhibitor to ameliorate renal cancer in a mouse model.

2014-01-06

Chatterjee S, Alsaeedi N, Hou J, Bandaru VV, Wu L, Halushka MK, Pili R, Ndikuyeze G, Haughey NJ. Use of a glycolipid inhibitor to ameliorate renal cancer in a mouse model. (2013) PLoS One. 8(5):e63726. PMID: 23671696; PMCID: PMC3650082.   Abstract In a xenograft model wherein, live renal cancer cells were implanted under the kidney capsule in mice, revealed a 30-fold...

Heparan sulfate deficiency disrupts developmental angiogenesis and causes congenital diaphragmatic hernia.

2014-01-02

Zhang B, Xiao W, Qiu H, Zhang F, Moniz HA, Jaworski A, Condac E, Gutierrez-Sanchez G, Heiss C, Clugston RD, Azadi P, Greer JJ, Bergmann C, Moremen KW, Li D, Linhardt RJ, Esko JD, Wang L. (2014) Heparan sulfate deficiency disrupts developmental angiogenesis and causes congenital...

Clinical characterization and survival of patients with borderline elevation in pulmonary artery pressure.

2013-12-12

Heresi GA, Minai OA, Tonelli AR, Hammel JP, Farha S, Parambil JG, Dweik RA. (2013) Clinical characterization and survival of patients with borderline elevation in pulmonary artery pressure. Pulm Circ. 3(4):916-25. PMID: 25006408. PMCID: PMC4070822. Abstract Normal resting mean pulmonary artery pressure (PAP) is 8-20 mmHg. Pulmonary...

Inducing host protection in pneumococcal sepsis by preactivation of the Ashwell-Morell receptor.

2013-12-10

Grewal PK, Aziz PV, Uchiyama S, Rubio GR, Lardone RD, Le D, Varki NM, Nizet V, Marth JD. (2013) Inducing host protection in pneumococcal sepsis by preactivation of the Ashwell-Morell receptor. Proc Natl Acad Sci U S A. 110(50):20218-23. PMID: 24284176. PMCID: PMC3864324. Abstract The endocytic Ashwell-Morell receptor...

Deficiency of gp91phox inhibits allergic airway inflammation.

2013-12-06

Sevin CM, Newcomb DC, Toki S, Han W, Sherrill TP, Boswell MG, Zhu Z, Collins RD, Boyd KL, Goleniewska K, Huckabee MM, Blackwell TS, Peebles RS Jr. (2013) Deficiency of gp91phox inhibits allergic airway inflammation. Am J Respir Cell Mol Biol. 49(3):396-402. PMID: 23590311 Abstract Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, a multienzyme complex, is the major source for production of reactive oxygen species...

Mass spectrometric analysis of sialylated glycans with use of solid-phase labeling of sialic acids.

2013-12-06

Shah P, Yang S, Sun S, Aiyetan P, Yarema KJ, Zhang H. (2013) Mass spectrometric analysis of sialylated glycans with use of solid-phase labeling of sialic acids. Anal Chem. 2;85(7):3606-13. PMID: 23445396; PMCID: PMC3681956 Abstract The analysis of sialylated glycans is critical for understanding the role of sialic acid in normal biological processes as well as in disease....

Analysis of N-glycoproteins using genomic N-glycosite prediction.

2013-12-06

Sun S, Zhang B, Aiyetan P, Zhou JY, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H. (2014) Analysis of N-glycoproteins using genomic N-glycosite prediction. J Proteome Res. 12:5609-15. PMID: 24164404. PMCID: PMC4072220. Abstract Protein glycosylation has long been recognized as one of the most...

Minimal effect of bortezomib in reducing anti-pig antibodies in human leukocyte antigen-sensitized patients: a pilot study

2013-11-19

Hara H, Bentall A, Long C, Fang J, Andreyev O, Lunz J, Ezzelarab M, Abu-Elmagd KM, Shapiro R, Ayares D, Stegall M, Cooper DK. (2013) Minimal effect of bortezomib in reducing anti-pig antibodies in human leukocyte antigen-sensitized patients: a pilot study. Xenotransplantation. 20:429-37. PMID: 23998233...

Solid phase extraction of N-linked glycopeptides using hydrazide tip.

2013-11-19

Chen J, Shah P, Zhang H. (2013) Solid phase extraction of N-linked glycopeptides using hydrazide tip. Anal Chem. 85(22):10670-4. PMID: 24079330. PMCID: PMC4082910. Abstract Glycoproteome contains valuable information where biomarkers may be discovered for disease diagnosis and monitoring. Nowadays, with the ever-increasing performances of mass spectrometers, the...

Molecular mass dependence of hyaluronan detection by sandwich ELISA-like assay and membrane blotting using biotinylated hyaluronan binding protein.

2013-11-15

Yuan H, Tank M, Alsofyani A, Shah N, Talati N, LoBello JC, Kim JR, Oonuki Y, de la Motte CA, Cowman MK. (2013) Molecular mass dependence of hyaluronan detection by sandwich ELISA-like assay and membrane blotting using biotinylated hyaluronan binding protein. Glycobiology. 23(11):1270-80. PMID: 23964097....

Glycogenomics as a mass spectrometry-guided genome-mining method for microbial glycosylated molecules.

2013-11-13

Kersten RD, Ziemert N, Gonzalez DJ, Duggan BM, Nizet V, Dorrestein PC, Moore BS. Glycogenomics as a mass spectrometry-guided genome-mining method for microbial glycosylated molecules. Proc Natl Acad Sci U S A. 110(47):E4407-16. PMID: 24191063; PMCID: PMC3839717 Abstract Glycosyl groups are an essential mediator of molecular interactions in cells and on cellular surfaces. There are very few...

Hyaluronic acid oligosaccharides suppress TLR3-dependent cytokine expression in a TLR4-dependent manner.

2013-11-12

Kim MY, Muto J, Gallo RL. (2013) Hyaluronic acid oligosaccharides suppress TLR3-dependent cytokine expression in a TLR4-dependent manner. PLoS One. 8(8):e72421. PMID: 24058413; PMCID: PMC3751928 Abstract The release of endogenous molecules from the skin after injury has been proposed to influence inflammation. Recent studies have found that pro-inflammatory signals can be generated by damaged...

Chemical approaches to study O-GlcNAcylation.

2013-11-12

Banerjee PS, Hart GW, Cho JW. (2013) Chemical approaches to study O-GlcNAcylation. Chem Soc Rev. 42(10):4345-57. PMID: 23247267; PMCID: PMC3641162 Abstract The enzymatic addition of a single β-D-N-acetylglucosamine sugar molecule on serine and/or threonine residues of protein chains is referred to as O-GlcNAcylation. This novel form of post-translational modification, first reported in 1984, is extremely...

Glycomic analysis by glycoprotein immobilization for glycan extraction and liquid chromatography on microfluidic chip.

2013-11-05

Yang S, Toghi Eshghi S, Chiu H, DeVoe DL, Zhang H. (2013) Glycomic analysis by glycoprotein immobilization for glycan extraction and liquid chromatography on microfluidic chip. Anal Chem. 85(21):10117-25. PMID: 24111616. PMCID: PMC3867136. Abstract Glycosylation is one of the most common protein modifications and profoundly regulates many...

Streptococcus pneumoniae invades erythrocytes and utilizes them to evade human innate immunity.

2013-10-23

Yamaguchi M, Terao Y, Mori-Yamaguchi Y, Domon H, Sakaue Y, Yagi T, Nishino K, Yamaguchi A, Nizet V, Kawabata S. (2013) Streptococcus pneumoniae invades erythrocytes and utilizes them to evade human innate immunity. PLoS One. 8(10):e77282. PMID: 24194877. PMCID: PMC3806730. Abstract Streptococcus pneumoniae, a Gram-positive bacterium, is...

Protein O-GlcNAcylation in diabetes and diabetic complications.

2013-10-23

Ma J, Hart GW. (2013) Protein O-GlcNAcylation in diabetes and diabetic complications. Expert Rev Proteomics. 10(4):365-80. PMID: 23992419 Abstract The post-translational modification of serine and threonine residues of proteins by O-linked β-N-acetylglucosamine (O-GlcNAc) is highly ubiquitous, dynamic and inducible. Protein O-GlcNAcylation serves as a key regulator of critical biological processes including...

Causes and circumstances of death in pulmonary arterial hypertension.

2013-10-22

Tonelli AR, Arelli V, Minai OA, Newman J, Bair N, Heresi GA, Dweik RA. (2013) Causes and circumstances of death in pulmonary arterial hypertension. Am J Respir Crit Care Med. 188(3):365-9. PMID: 23600433; PMCID: PMC3778730   Abstract RATIONALE: The causes and circumstances surrounding death are understudied in patients with pulmonary arterial hypertension (PAH). OBJECTIVES: We sought to determine the specific reasons and characteristics...

Detection of O-GlcNAc modifications on cardiac myofilament proteins.

2013-10-20

Ramirez-Correa GA, Ferrando IM, Hart G, Murphy A. (2013) Detection of O-GlcNAc modifications on cardiac myofilament proteins. Methods Mol Biol. 2013;1005:157-68. doi: 10.1007/978-1-62703-386-2_13. PMID: 23606256. Abstract In this chapter it is described a general method that has been used successfully by more than one laboratory interested in...

Human milk hyaluronan enhances innate defense of the intestinal epithelium.

2013-10-04

Hill DR, Rho HK, Kessler SP, Amin R, Homer CR, McDonald C, Cowman MK, de la Motte CA. (2013) Human milk hyaluronan enhances innate defense of the intestinal epithelium. J Biol Chem. 288(40):29090-104. PMID: 23950179. PMCID: PMC3790008. Abstract Breast-feeding is associated with enhanced protection from gastrointestinal disease...

Diabetic hyperglycaemia activates CaMKII and arrhythmias by O-linked glycosylation.

2013-10-02

Erickson JR, Pereira L, Wang L, Han G, Ferguson A, Dao K, Copeland RJ, Despa F, Hart GW, Ripplinger CM, Bers DM. (2013) Nature. 502(7471):372-6. PMID: 24077098; PMCID: PMC3801227 Abstract Ca2+/calmodulin-dependent protein kinase II (CaMKII) is an enzyme with important regulatory functions in the heart and brain, and its chronic activation can be pathological. CaMKII activation is seen in...

EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and α1-acid glycoprotein.

2013-10-01

Sjögren J, Struwe WB, Cosgrave EF, Rudd PM, Stervander M, Allhorn M, Hollands A, Nizet V, Collin M. (2013) EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and α1-acid glycoprotein. Biochem J. 455(1):107-18. PMID:...

Human T cells upregulate CD69 after coculture with xenogeneic genetically-modified pig mesenchymal stromal cells.

2013-09-30

Li J, Andreyev O, Chen M, Marco M, Iwase H, Long C, Ayares D, Shen Z, Cooper DK, Ezzelarab MB. (2013) Human T cells upregulate CD69 after coculture with xenogeneic genetically-modified pig mesenchymal stromal cells. Cell Immunol. 285(1-2):23-30. PMID: 24044963   Abstract Mesenchymal stromal cells (MSC) obtained from α1,3-galactosyltransferase gene knock-out pigs transgenic for the human complement-regulatory protein CD46 (GTKO/CD46 pMSC) suppress...

Polysomnographic determinants of nocturnal hypercapnia in patients with sleep apnea.

2013-09-25

Jaimchariyatam N, Dweik RA, Kaw R, Aboussouan LS. (2013) Polysomnographic determinants of nocturnal hypercapnia in patients with sleep apnea. J Clin Sleep Med. 15;9(3):209-15. PMID: 23493528; PMCID: PMC3578680 Abstract STUDY OBJECTIVES: Identify polysomnographic and demographic factors associated with elevation of nocturnal end-tidal CO2 in patients with obstructive sleep apnea. METHODS: Forty-four adult patients with obstructive sleep apnea were selected...

Toward a platform for comprehensive glycan sequencing.

2013-09-24

Reinhold V, Zhang H, Hanneman A, Ashline D. (2013) Toward a platform for comprehensive glycan sequencing. Mol Cell Proteomics. 12(4):866-73. PMID: 23438731; PMCID: PMC3617333 Abstract From a series of recently published reports, an analytical platform has been proposed for a quantitative and qualitative measure of N- and O-glycosylation, complete with peptide-glycan connectivity and detailed structural understanding....

Enhanced T cell function in a mouse model of human glycosylation.

2013-09-24

Buchlis G, Odorizzi P, Soto PC, Pearce OM, Hui DJ, Jordan MS, Varki A, Wherry EJ, High KA. (2013) Enhanced T cell function in a mouse model of human glycosylation. J Immunol. 191(1):228-37. PMID: 23709682; PMCID: PMC3691298 Abstract Clinical evidence for a more active immune response in humans compared with our closest hominid relative, the chimpanzee, includes the progression of HIV...

Loss of Siglec-14 reduces the risk of chronic obstructive pulmonary disease exacerbation.

2013-09-22

Angata T, Ishii T, Motegi T, Oka R, Taylor RE, Soto PC, Chang YC, Secundino I, Gao CX, Ohtsubo K, Kitazume S, Nizet V, Varki A, Gemma A, Kida K, Taniguchi N. (2013) Loss of Siglec-14 reduces the risk of chronic obstructive pulmonary disease exacerbation. Cell Mol Life Sci. 70(17):3199-210. PMID: 23519826; PMCID: PMC3718857 Abstract Chronic obstructive pulmonary disease (COPD) is a leading cause of mortality...

Designing allosteric regulators of thrombin. Exosite 2 features multiple subsites that can be targeted by sulfated small molecules for inducing inhibition.

2013-09-19

Sidhu PS, Abdel Aziz MH, Sarkar A, Mehta AY, Zhou Q, Desai UR. Designing allosteric regulators of thrombin. Exosite 2 features multiple subsites that can be targeted by sulfated small molecules for inducing inhibition. (2013) J Med Chem. 27;56(12):5059-70. PMID: 23718540; PMCID: PMC3717983 Abstract We recently designed a group of novel exosite-2-directed sulfated, small, allosteric inhibitors of thrombin....

Human HDL containing a novel apoC-I isoform induces smooth muscle cell apoptosis.

2013-09-17

McNeal CJ, Chatterjee S, Hou J, Worthy LS, Larner CD, Macfarlane RD, Alaupovic P, Brocia RW. (2013) Human HDL containing a novel apoC-I isoform induces smooth muscle cell apoptosis. Cardiovasc Res. 1;98(1):83-93 PMID: 23354389; PMCID: PMC3598419   Abstract AIMS: We discovered that some adults with coronary heart disease (CHD) have a high density lipoprotein (HDL) subclass which induces human aortic smooth muscle...

Human Milk Oligosaccharides Protect Bladder Epithelial Cells Against Uropathogenic Escherichia coli Invasion and Cytotoxicity.

2013-09-07

Lin AE, Autran CA, Espanola SD, Bode L, Nizet V. (2014) Human Milk Oligosaccharides Protect Bladder Epithelial Cells Against Uropathogenic Escherichia coli Invasion and Cytotoxicity. J Infect Dis. 209(3):323-4. PMID: 23990566 PMCID: PMC3883170.   Abstract The invasive pathogen Uropathogenic E. coli (UPEC) is the primary cause of urinary tract infections (UTI). Recurrent infection that can progress to life-threatening renal...

Glycan analysis by isobaric aldehyde reactive tags and mass spectrometry.

2013-09-03

Yang S, Yuan W, Yang W, Zhou J, Harlan R, Edwards J, Li S, Zhang H. (2013) Glycan analysis by isobaric aldehyde reactive tags and mass spectrometry. Anal Chem. 85(17):8188-95. PMID: 23895018. PMCID: PMC3785247. Abstract Glycans play significant roles in physiological and pathological processes. Therefore, quantitative analysis...

Mice deficient in the St3gal3 gene product α2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation.

2013-08-28

Kiwamoto T, Brummet ME, Wu F, Motari MG, Smith DF, Schnaar RL, Zhu Z, Bochner BS. (2013) Mice deficient in the St3gal3 gene product α2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation. J Allergy Clin Immunol. 133(1):240-7.e1-3. PMID: 23830412 PMCID: PMC3874253.   Abstract BACKGROUND: Sialic acid-binding immunoglobulin-like lectin (Siglec)-F is a proapoptotic receptor on mouse eosinophils, but little is...

Sialyltransferase ST3Gal-III regulates Siglec-F ligand formation and eosinophilic lung inflammation in mice.

2013-08-20

Suzukawa M, Miller M, Rosenthal P, Cho JY, Doherty TA, Varki A, Broide D. (2013) Sialyltransferase ST3Gal-III regulates Siglec-F ligand formation and eosinophilic lung inflammation in mice. J Immunol. 190(12):5939-48. PMID: 23677475; PMCID: PMC3679360   Abstract Sialic acid-binding, Ig-like lectin (Siglec)-F is highly expressed on mouse eosinophils and plays an important role in regulating levels of eosinophilic lung inflammation. In...

Glycosylation Network Analysis Toolbox: a MATLAB-based environment for systems glycobiology.

2013-08-19

Liu G, Puri A, Neelamegham S. (2013) Glycosylation Network Analysis Toolbox: a MATLAB-based environment for systems glycobiology. Bioinformatics. 29(3):404-6. PMID: 23230149; PMCID: PMC3562062   Abstract Systems glycobiology studies the interaction of various pathways that regulate glycan biosynthesis and function. Software tools for the construction and analysis of such pathways are not yet available. We present GNAT,...

Activation of TLR3 in keratinocytes increases expression of genes involved in formation of the epidermis, lipid accumulation, and epidermal organelles.

2013-08-08

Borkowski AW, Park K, Uchida Y, Gallo RL. (2013) Activation of TLR3 in keratinocytes increases expression of genes involved in formation of the epidermis, lipid accumulation, and epidermal organelles. J Invest Dermatol. 133(8):2031-40. PMID: 23353987. PMCID: PMC3686920. Abstract Injury to the skin, and the subsequent release of...

Cell-intrinsic mechanism involving Siglec-5 associated with divergent outcomes of HIV-1 infection in human and chimpanzee CD4 T cells.

2013-08-05

Soto PC, Karris MY, Spina CA, Richman DD, Varki A. (2013) Cell-intrinsic mechanism involving Siglec-5 associated with divergent outcomes of HIV-1 infection in human and chimpanzee CD4 T cells. J Mol Med (Berl). 91(2):261-70. PMID: 22945238; PMCID: PMC3558668 Abstract Human and chimpanzee CD4+ T cells differ markedly in expression of the inhibitory receptor Siglec-5, which contributes towards...

The role of lung epithelial ligands for Siglec-8 and Siglec-F in eosionophilic inflammation.

2013-08-05

Kiwamoto, T., Katoh, T., Tiemeyer, M., Bochner,BS. (2013) The role of lung epithelial ligands for Siglec-8 and Siglec-F in eosionophilic inflammation. Curr Opin Allergy Clin Immunol. 13(1):106-11. PMID: 23160308 Abstract PURPOSE OF REVIEW: Siglec-8 and Siglec-F are single pass transmembrane inhibitory receptors found on the surface of human and mouse eosinophils,...

Biological functions of ecto-enzymes in regulating extracellular adenosine levels in neoplastic and inflammatory disease states.

2013-08-05

Longhi MS, Robson SC, Bernstein SH, Serra S, Deaglio S. (2013) Biological functions of ecto-enzymes in regulating extracellular adenosine levels in neoplastic and inflammatory disease states. J Mol Med (Berl). 91(2):165-72. PMID: 23292173 Abstract When present in the extracellular environment, the nucleoside adenosine protects cells and tissues from excessive inflammation and immune-mediated damage while promoting healing...

New Concepts of Immune Modulation in Xenotransplantation.

2013-08-05

Satyananda V, Hara H, Ezzelarab MB, Phelps C, Ayares D, Cooper DK. (2013) New Concepts of Immune Modulation in Xenotransplantation. Transplantation. 96(11):937-45. PMID: 23851935 Abstract The shortage of human organs for transplantation has focused research on the possibility of transplanting pig organs into humans. Many factors contribute to the failure of a pig organ graft in...

Characterization of disease-associated N-linked glycoproteins.

2013-08-05

Tian Y, Zhang H. (2013) Characterization of disease-associated N-linked glycoproteins. Proteomics. 13(3-4):504-11. PMID: 23255236; PMCID: PMC3733363 Abstract N-linked glycoproteins play important roles in biological processes, including cell-to-cell recognition, growth, differentiation, and programmed cell death. Specific N-linked glycoprotein changes are associated with disease progression and identification of these N-linked glycoproteins has potential for use in...

Impact of O-GlcNAc on cardioprotection by remote ischaemic preconditioning in non-diabetic and diabetic patients.

2013-08-05

Jensen RV, Zachara NE, Nielsen PH, Kimose HH, Kristiansen SB, Bøtker HE. (2013) Impact of O-GlcNAc on cardioprotection by remote ischaemic preconditioning in non-diabetic and diabetic patients. Cardiovasc Res. 97(2):369-78. PMID: 23201773; PMCID: PMC3584969   Abstract AIMS: Post-translational modification of proteins by O-linked β-N-acetylglucosamine (O-GlcNAc) is cardioprotective but its role in cardioprotection by remote ischaemic preconditioning (rIPC) and the reduced...

The promise of sulfated synthetic small molecules as modulators of glycosaminoglycan function.

2013-08-05

Desai UR. (2013) The promise of sulfated synthetic small molecules as modulators of glycosaminoglycan function. Future Med Chem. 5:1363-1366. PMID: 23919545. Link to journal: http://www.future-science.com/loi/fmc

Overcoming neurite-inhibitory chondroitin sulfate proteoglycans in the astrocyte matrix.

2013-06-08

Cua RC, Lau LW, Keough MB, Midha R, Apte SS, Yong VW. (2013) Overcoming neurite-inhibitory chondroitin sulfate proteoglycans in the astrocyte matrix. Glia.61(6):972-84. PMID: 23554135. Abstract Acute trauma to the central nervous system (CNS) can result in permanent damage and loss of function related to the poor...

The biology of the extracellular matrix: novel insights.

2013-06-03

Hubmacher D, Apte SS. (2013) The biology of the extracellular matrix: novel insights. Curr Opin Rheumatol. 25(1):65-70. PMID: 23143224. PMCID: PMC3560377.   Abstract PURPOSE OF REVIEW: Extracellular matrix (ECM) has both structural and regulatory roles. This update reviews the representative recent developments in diverse aspects of ECM biology relevant to inflammation, tissue destruction,...

Glycomic Analysis Using Glycoprotein Immobilization for Glycan Extraction.

2013-06-03

Yang S, Li Y, Shah P, Zhang H. (2013) Glycomic Analysis Using Glycoprotein Immobilization for Glycan Extraction. Anal Chem. 85(11):5555-61. PMID: 23688297; PMCID: PMC3696186 Abstract Glycosylation is one of the most common protein modifications and is involved in many functions of glycoproteins. Investigating aberrant protein glycosylation associated with diseases is useful in improving disease diagnostics....

Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation.

2013-05-07

Kawasaki N, Vela JL, Nycholat CM, Rademacher C, Khurana A, van Rooijen N, Crocker PR, Kronenberg M, Paulson JC. (2013) Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation. Proc Natl Acad Sci U S A. 110(19):7826-31. PMID: 23610394; PMCID: PMC3651435   Abstract Invariant natural killer T (iNKT) cells...

On-Chip Synthesis and Screening of a Sialoside Library Yields a High Affinity Ligand for Siglec-7.

2013-05-06

Rillahan CD, Schwartz E, Rademacher C, McBride R, Rangarajan J, Fokin VV, Paulson JC. (2013) On-Chip Synthesis and Screening of a Sialoside Library Yields a High Affinity Ligand for Siglec-7. ACS Chem Biol. PMID: 23597400; PMCID: PMC3751994 Abstract The Siglec family of sialic acid-binding proteins are differentially expressed on white blood cells of the immune system and represent...

O-GlcNAcomics-Revealing roles of O-GlcNAcylation in disease mechanisms and development of potential diagnostics.

2013-05-03

Copeland RJ, Han G, Hart GW. (2013) O-GlcNAcomics-Revealing roles of O-GlcNAcylation in disease mechanisms and development of potential diagnostics. Proteomics Clin Appl. 2013 May 3. doi: 10.1002/prca.201300001. PMID: 23640805. PMCID: PMC3883918. Abstract O-linked-β-N-acetylglucosamine (O-GlcNAc) is a dynamic PTM of the 3'-hydroxyl groups of serine or threonine residues...

Novel low molecular weight lignins as potential anti-emphysema agents: In vitro triple inhibitory activity against elastase, oxidation and inflammation.

2013-04-30

Saluja B, Thakkar JN, Li H, Desai UR, Sakagami M. (2013) Novel low molecular weight lignins as potential anti-emphysema agents: In vitro triple inhibitory activity against elastase, oxidation and inflammation. Pulm Pharmacol Ther. 26(2):296-304 doi: 10.1016/j.pupt.2012.12.009. PMID: 23280431; PMCID: PMC3581723   Abstract No molecule has been found to be effective against emphysema to date primarily...

Chemokine CXCL1 dimer is a potent agonist for the CXCR2 receptor.

2013-04-26

Ravindran A, Sawant KV, Sarmiento J, Navarro J, Rajarathnam K. (2013) Chemokine CXCL1 dimer is a potent agonist for the CXCR2 receptor. J Biol Chem. 288(17):12244-52. PMID: 23479735; PMCID: PMC3636908 Abstract The CXCL1/CXCR2 axis plays a crucial role in recruiting neutrophils in response to microbial infection and tissue injury, and dysfunction in this process has been implicated...

Real-time kinetics of high-mobility group box 1 (HMGB1) oxidation in extracellular fluids studied by in situ protein NMR spectroscopy.

2013-04-26

Zandarashvili L, Sahu D, Lee K, Lee YS, Singh P, Rajarathnam K, Iwahara J. (2013) Real-time kinetics of high-mobility group box 1 (HMGB1) oxidation in extracellular fluids studied by in situ protein NMR spectroscopy. J Biol Chem. 288(17):11621-7. PMID: 23447529; PMCID: PMC3636853 Abstract Some extracellular proteins are initially secreted in reduced forms via a non-canonical pathway bypassing the endoplasmic...

Versican processing by a disintegrin-like and metalloproteinase domain with thrombospondin-1 repeats proteinases-5 and -15 facilitates myoblast fusion.

2013-04-22

Stupka N, Kintakas C, White JD, Fraser FW, Hanciu M, Aramaki-Hattori N, Martin S, Coles C, Collier F, Ward AC, Apte SS, McCulloch DR. (2013) Versican processing by a disintegrin-like and metalloproteinase domain with thrombospondin-1 repeats proteinases-5 and -15 facilitates myoblast fusion. J Biol Chem. 288(3):1907-17. PMID: 23233679; PMCID: PMC3548499   Abstract Skeletal muscle development and regeneration requires the fusion of myoblasts into multinucleated myotubes....

Response.

2013-04-22

Tonelli AR, Dweik RA. (2013) Response. Chest. 143(1):273-4. PMID: 23276860; PMCID: PMC3610693 Comment on Prevalence and prognostic value of left ventricular diastolic dysfunction in idiopathic and heritable pulmonary arterial hypertension. [Chest. 2012] Diastolic dysfunction with nondilated left atrium. [Chest. 2013] Link to journal: http://journal.publications.chestnet.org/

Epicutaneous exposure to staphylococcal superantigen enterotoxin B enhances allergic lung inflammation via an IL-17A dependent mechanism.

2013-04-22

Yu J, Oh MH, Park JU, Myers AC, Dong C, Zhu Z, Zheng T. (2012) Epicutaneous exposure to staphylococcal superantigen enterotoxin B enhances allergic lung inflammation via an IL-17A dependent mechanism. PLoS One. 7(7):e39032. PMID: 22848348; PMCID: PMC3407176 Abstract Atopic dermatitis (AD) is the initial step of the atopic march: the progression from AD to allergic rhinitis and asthma....

Single exhaled breath metabolomic analysis identifies unique breathprint in patients with acute decompensated heart failure.

2013-04-02

Samara MA, Tang WH, Cikach F Jr, Gul Z, Tranchito L, Paschke KM, Viterna J, Wu Y, Laskowski D, Dweik RA. (2013) Single exhaled breath metabolomic analysis identifies unique breathprint in patients with acute decompensated heart failure. J Am Coll Cardiol. 61(13):1463-4. PMID: 23500243; PMCID: PMC3760473    No Abstract Available Link to journal: http://content.onlinejacc.org/journal.aspx

SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.

2013-03-25

Zhou L, Oh SY, Zhou Y, Yuan B, Wu F, Oh MH, Wang Y, Takemoto C, Van Rooijen N, Zheng T, Zhu Z. (2013) SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis. PLoS One. 8(2):e55763. PMID: 23390550; PMCID: PMC3563592   Abstract Allergic inflammation and severe allergic reactions (anaphylaxis) are important in allergen induced diseases. Bacterial products such as...

Discovery of Allosteric Modulators of Factor XIa by Targeting Hydrophobic Domains Adjacent to its Heparin-Binding Site.

2013-03-19

Karuturi R, Al-Horani RA, Mehta SC, Gailani D, Desai UR. (2013) Discovery of Allosteric Modulators of Factor XIa by Targeting Hydrophobic Domains Adjacent to its Heparin-Binding Site. J Med Chem. PMID: 23451707. PMCID: PMC3625964 Abstract To discover promising sulfated allosteric modulators (SAMs) of glycosaminoglycan-binding proteins (GBPs), such as human factor XIa (FXIa), we screened a library...

Extracellular and intracellular esterase processing of SCFA-hexosamine analogs: implications for metabolic glycoengineering and drug delivery.

2013-03-19

Mathew MP, Tan E, Shah S, Bhattacharya R, Adam Meledeo M, Huang J, Espinoza FA, Yarema KJ. (2012) Extracellular and intracellular esterase processing of SCFA-hexosamine analogs: implications for metabolic glycoengineering and drug delivery. Bioorg Med Chem Lett. 22(22):6929-33. PMID: 23041156. PMCID: PMC3530194 Abstract This report provides a synopsis of the esterase processing of short chain fatty acid (SCFA)-derivatized hexosamine...

Irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6.

2013-03-14

Lauer ME, Glant TT, Mikecz K, DeAngelis PL, Haller FM, Husni ME, Hascall VC, Calabro A. (2013) Irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6. J Biol Chem. 288(1):205-14. PMID: 23166324. PMCID: PMC3537015   Abstract The covalent transfer of heavy chains (HCs) from inter-α-inhibitor (IαI) to hyaluronan (HA)...

Tumor necrosis factor-stimulated gene-6 (TSG-6) amplifies hyaluronan synthesis by airway smooth muscle cells.

2013-03-14

Lauer ME, Cheng G, Swaidani S, Aronica MA, Weigel PH, Hascall VC. (2013) Tumor necrosis factor-stimulated gene-6 (TSG-6) amplifies hyaluronan synthesis by airway smooth muscle cells. J Biol Chem. 288(1):423-31. PMID: 23129777. PMCID: PMC3537040   Abstract We tested the hypothesis that the artificial addition of heavy chains from inter-α-inhibitor to hyaluronan (HA), by adding...

Targeted analyte detection by standard addition improves detection limits in matrix-assisted laser desorption/ionization mass spectrometry.

2013-03-13

Toghi Eshghi S, Li X, Zhang H. (2012) Targeted analyte detection by standard addition improves detection limits in matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem. 84(18):7626-32. PMID: 22877355   Abstract Matrix-assisted laser desorption/ionization (MALDI) has proven an effective tool for fast and accurate determination of many molecules. However, the detector sensitivity and...

Loss of β-catenin induces multifocal periosteal chondroma-like masses in mice.

2013-03-09

Cantley L, Saunders C, Guttenberg M, Candela ME, Ohta Y, Yasuhara R, Kondo N, Sgariglia F, Asai S, Zhang X, Qin L, Hecht JT, Chen D, Yamamoto M, Toyosawa S, Dormans JP, Esko JD, Yamaguchi Y, Iwamoto M, Pacifici M, Enomoto-Iwamoto M. (2013) Loss of...

Sulfated Pentagalloylglucoside Is a Potent, Allosteric, and Selective Inhibitor of Factor XIa.

2013-01-30

Al-Horani RA, Ponnusamy P, Mehta AY, Gailani D, Desai UR. (2013) Sulfated Pentagalloylglucoside Is a Potent, Allosteric, and Selective Inhibitor of Factor XIa. J Med Chem. 56(3):867-78. PMID: 23316863; PMCID: PMC3574227   Abstract Inhibition of factor XIa (FXIa) is a novel paradigm for developing anticoagulants without major bleeding consequences. We present the discovery of sulfated pentagalloylglucoside (6) as...

The biology of the extracellular matrix: novel insights.

2013-01-22

Hubmacher D, Apte SS. (2013)  The biology of the extracellular matrix: novel insights. Curr Opin Rheumatol. 25(1):65-70. PMID: 23143224; PMCID: PMC3560377 Abstract PURPOSE OF REVIEW: Extracellular matrix (ECM) has both structural and regulatory roles. This update reviews the representative recent developments in diverse aspects of ECM biology relevant to inflammation, tissue destruction, fibrosis, and regeneration. RECENT FINDINGS: Biological...

Nitric oxide deficiency in pulmonary hypertension: Pathobiology and implications for therapy.

2013-01-12

Tonelli AR, Haserodt S, Aytekin M, Dweik RA. (2013) Nitric oxide deficiency in pulmonary hypertension: Pathobiology and implications for therapy. Pulm Circ. 3(1):20-30. PMID: 23662172. PMCID: PMC3641730. Abstract Nitric oxide (NO) is a diffusible gas with diverse roles in human physiology and disease. Significant progress in the...

Correlation of hyaluronan deposition with infiltration of eosinophils and lymphocytes in a cockroach-induced murine model of asthma.

2013-01-09

Cheng G, Swaidani S, Sharma M, Lauer ME, Hascall VC, Aronica MA. (2013) Correlation of hyaluronan deposition with infiltration of eosinophils and lymphocytes in a cockroach-induced murine model of asthma. Glycobiology. 23(1):43-58. PMID: 22917573. PMCID: PMC3505010. Abstract Asthma is a chronic inflammatory disease that exhibits airway remodeling...

Modification of RelA by O-linked N-acetylglucosamine links glucose metabolism to NF-kB acetylation and transcription.

2013-01-08

Allison DF, Wamsley JJ, Kumar M, Li D, Gray LG, Hart GW, Jones DR, Mayo MW. (2012) Modification of RelA by O-linked N-acetylglucosamine links glucose metabolism to NF-κB acetylation and transcription. Proc Natl Acad Sci U S A. 109(42):16888-93. PMID: 23027940. PMCID: PMC3479489   Abstract The molecular mechanisms linking glucose metabolism with active transcription remain undercharacterized in mammalian cells. Using nuclear...

TSG-6 protein is crucial for the development of pulmonary hyaluronan deposition, eosinophilia, and airway hyperresponsiveness in a murine model of asthma.

2013-01-04

Swaidani S, Cheng G, Lauer ME, Sharma M, Mikecz K, Hascall VC, Aronica MA. (2013) TSG-6 protein is crucial for the development of pulmonary hyaluronan deposition, eosinophilia, and airway hyperresponsiveness in a murine model of asthma. J Biol Chem. 288(1):412-22. PMID: 23118230. PMCID: PMC3537038. Abstract Hyaluronan (HA)...

A genetic model of substrate reduction therapy for mucopolysaccharidosis.

2013-01-02

Lamanna WC, Lawrence R, Sarrazin S, Lameda-Diaz C, Gordts PL, Moremen KW, Esko JD. (2012) A genetic model of substrate reduction therapy for mucopolysaccharidosis. J Biol Chem. 287(43):36283-90. PMID: 22952226. PMCID: PMC3476295   Abstract Inherited defects in the ability to catabolize glycosaminoglycans result in lysosomal storage disorders known as mucopolysaccharidoses (MPS), causing severe pathology, particularly in the brain. Enzyme replacement...

On scaffold hopping: Challenges in the discovery of sulfated small molecules as mimetics of glycosaminoglycans.

2012-12-03

Sidhu PS, Mosier PD, Zhou Q, Desai UR. (2012) On scaffold hopping: Challenges in the discovery of sulfated small molecules as mimetics of glycosaminoglycans. Bioorg Med Chem Lett. pii: S0960-894X(12)01370-4. PMID: 23164711. PMCID: PMC3525718   Abstract The design of sulfated, small, nonsaccharide molecules as modulators of proteins is still in...

Silencing α1,3-fucosyltransferases in human leukocytes reveals a role for FUT9 during E-selectin mediated cell adhesion

2012-11-28

Buffone A Jr, Mondal N, Gupta R, McHugh KP, Lau JT, Neelamegham S. (2013) Silencing α1,3-fucosyltransferases in human leukocytes reveals a role for FUT9 during E-selectin mediated cell adhesion. J Biol Chem. 288(3):1620-1633. PMID: 23192350; PMCID: PMC3548472   Abstract Leukocyte adhesion during inflammation is initiated by the binding...

Workshop report from the National Institutes of Health Taskforce on the Research Needs of Eosinophil-Associated Diseases (TREAD).

2012-11-13

Bochner BS, Book W, Busse WW, Butterfield J, Furuta GT, Gleich GJ, Klion AD, Lee JJ, Leiferman KM, Minnicozzi M, Moqbel R, Rothenberg ME, Schwartz LB,Simon HU, Wechsler ME, Weller PF. (2012) Workshop report from the National Institutes of Health Taskforce on the Research Needs of Eosinophil-Associated Diseases (TREAD). J Allergy Clin Immunol. 130(3):587-96. PMID: 22935587. PMCID: PMC3432981   Abstract BACKGROUND: Eosinophils are blood cells that are often found in...

The phagocytic capacity and immunological potency of human dendritic cells is improved by α2,6-sialic acid deficiency.

2012-11-13

Cabral MG, Silva Z, Ligeiro D, Seixas E, Crespo H, Carrascal MA, Silva M, Piteira AR, Paixão P, Lau JT, Videira PA. (2012) The phagocytic capacity and immunological potency of human dendritic cells is improved by α2,6-sialic acid deficiency. Immunology. 138(3):235-245. PMID: 23113614; PMCID: PMC3573277   Abstract Dendritic cells (DCs) play an essential role in immunity against bacteria by phagocytosing and eliciting adaptive...

Inactivation of heparan sulfate 2-O-sulfotransferase accentuates neutrophil infiltration during acute inflammation in mice.

2012-11-06

Axelsson J, Xu D, Kang BN, Nussbacher JK, Handel TM, Ley K, Sriramarao P, Esko JD. (2012) Inactivation of heparan sulfate 2-O-sulfotransferase accentuates neutrophil infiltration during acute inflammation in mice. Blood. 120(8):1742-51. PMID: 22791291. PMCID: PMC3429312 Abstract Neutrophil recruitment and extravasation at sites of inflammation provide a mechanism...

Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis.

2012-11-06

Almaraz RT, Tian Y, Bhattarcharya R, Tan E, Chen SH, Dallas MR, Chen L, Zhang Z, Zhang H, Konstantopoulos K, Yarema KJ.  (2012) Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis. Mol Cell Proteomics. 11(7):M112.017558. PMID: 22457533, PMCID: PMC3394959 Abstract This study reports a global glycoproteomic analysis...

Click and pick: identification of sialoside analogues for siglec-based cell targeting.

2012-10-26

Rillahan CD, Schwartz E, McBride R, Fokin VV, Paulson JC. (2012) Click and pick: identification of sialoside analogues for siglec-based cell targeting. Angew Chem Int Ed Engl. 51(44):11014-8. doi: 10.1002/anie.201205831. PMID: 23038623. PMCID: PMC3480989 Abstract Click 'n' chips: Azide and alkyne-bearing sialic acids (purple diamond; see picture) were subjected...

Biomarkers of eosinophil involvement in allergic and eosinophilic diseases: review of phenotypic and serum markers including a novel assay to quantify levels of soluble Siglec-8.

2012-09-28

Na HJ, Hamilton RG, Klion AD, Bochner BS. (2012) Biomarkers of eosinophil involvement in allergic and eosinophilic diseases: review of phenotypic and serum markers including a novel assay to quantify levels of soluble Siglec-8. J Immunol Methods. 383:39-46. PMID: 22683541. PMCID: PMC3411856. Abstract There remains considerable controversy...

In silico-aided design of a glycan ligand of sialoadhesin for in vivo targeting of macrophages.

2012-09-26

Nycholat CM, Rademacher C, Kawasaki N, Paulson JC. (2012) In silico-aided design of a glycan ligand of sialoadhesin for in vivo targeting of macrophages. J Am Chem Soc. 134:15696-9. PMID: 22967315 PMCID: PMC3462166. Abstract Cell-specific delivery of therapeutic agents using ligand targeting is gaining interest because of...

Siglec-8 as a drugable target to treat eosinophil and mast cell-associated conditions.

2012-09-12

Kiwamoto T, Kawasaki N, Paulson JC, Bochner BS. (2012) Siglec-8 as a drugable target to treat eosinophil and mast cell-associated conditions. Pharmacol Ther. 135:327-36. PMID: 22749793. Abstract Siglecs (sialic acid immunoglobulin-like lectins) are members of the immunoglobulin gene family that contain sialoside binding N-terminal domains. They are...

A Conserved UDP-glucose Dehydrogenase Outside the hasABC Operon Contributes to Capsule Biogenesis in Group A Streptococcus.

2012-09-07

Cole JN, Aziz RK, Kuipers K, Timmer AM, Nizet V, van Sorge NM. (2012) A Conserved UDP-glucose Dehydrogenase Outside the hasABC Operon Contributes to Capsule Biogenesis in Group A Streptococcus. J Bacteriol. 194(22):6154-61. PMID: 22961854; PMCID: PMC3486382 Abstract Group A Streptococcus (GAS) is a human-specific bacterial pathogen responsible...

Evolution of siglec-11 and siglec-16 genes in hominins.

2012-08-29

Wang X, Mitra N, Cruz P, Deng L; NISC Comparative Sequencing Program, Varki N, Angata T, Green ED, Mullikin J, Hayakawa T, Varki A. (2012) Evolution of siglec-11 and siglec-16 genes in hominins. Mol Biol Evol. 29:2073-86. PMID: 22383531. PMCID: PMC3408085 Abstract We previously reported a human-specific...

Potential factors influencing the development of thrombocytopenia and consumptive coagulopathy after genetically modified pig liver xenotransplantation.

2012-08-26

Ekser B, Lin CC, Long C, Echeverri GJ, Hara H, Ezzelarab M, Bogdanov VY, Stolz DB, Enjyoji K, Robson SC, Ayares D, Dorling A, Cooper DK, Gridelli B. Potential factors influencing the development of thrombocytopenia and consumptive coagulopathy after genetically modified pig liver xenotransplantation. Transpl...

Ultraviolet radiation damages self noncoding RNA and is detected by TLR3.

2012-08-12

Bernard JJ, Cowing-Zitron C, Nakatsuji T, Muehleisen B, Muto J, Borkowski AW, Martinez L, Greidinger EL, Yu BD, Gallo RL. (2012) Ultraviolet radiation damages self noncoding RNA and is detected by TLR3. Nat Med. 18(8):1286-90. PMID: 22772463. PMCID: PMC3812946. Abstract Exposure to ultraviolet B (UVB) radiation from...

Designing allosteric regulators of thrombin. Monosulfated benzofuran dimers selectively interact with arg173 of exosite II to induce inhibition

2012-08-09

Abdel Aziz MH, Sidhu PS, Liang A, Kim JY, Mosier PD, Zhou Q, Farrell DH, Desai UR. (2012) Designing allosteric regulators of thrombin. Monosulfated benzofuran dimers selectively interact with arg173 of exosite II to induce inhibition. J Med Chem. 55(15):6888-97. PMID: 22788964. PMCID: PMC3416887. Abstract Earlier, we...

Potent direct inhibitors of factor Xa based on the tetrahydroisoquinoline scaffold.

2012-08-01

Al-Horani RA, Meta AY, Desai UR. (2012) Potent direct inhibitors of factor Xa based on the tetrahydroisoquinoline scaffold. Eur J Med Chem. 54:771-83. PMID: 22770607; PMCID: PMC3408863. Abstract Direct inhibition of coagulation factor Xa (FXa) carries significant promise for developing effective and safe anticoagulants. Although a large number...

The antimicrobial protein REG3A regulates keratinocyte proliferation and differentiation after skin injury.

2012-07-27

Lai Y, Li D, Li C, Muehleisen B, Radek KA, Park HJ, Jiang Z, Li Z, Lei H, Quan Y, Zhang T, Wu Y, Kotol P, Morizane S, Hata TR, Iwatsuki K, Tang C, Gallo RL. (2012) The antimicrobial protein REG3A regulates keratinocyte proliferation and...

Platelet aggregation in humans and nonhuman primates: relevance to xenotransplantation.

2012-07-26

Iwase H, Ekser B, Zhou H, Dons EM, Cooper DK, Ezzelarab MB. (2012) Platelet aggregation in humans and nonhuman primates: relevance to xenotransplantation. Xenotransplantation. 19(4):233-43. doi: 10.1111/j.1399-3089.2012.00712.x. PMID: 22909136. PMCID: PMC3425958. Abstract INTRODUCTION: Platelet activation/aggregation plays a key role in the dysregulation of coagulation and the development of...

Cardiovascular biomarkers in exhaled breath.

2012-07-01

Cikach FS Jr, Dweik RA. (2012) Cardiovascular biomarkers in exhaled breath. Prog Cardiovasc Dis. 55(1):34-43. PMID: 22824108. Abstract With each breath that we exhale, thousands of molecules are expelled in our breath, giving individuals a "breath-print" that can tell a lot about them and their state of health....

Discovery of novel sulfonated small molecules that inhibit vascular tube formation.

2012-07-01

Raman K, Karuturi R, Swarup VP, Desai UR, Kuberan B. (2012) Discovery of novel sulfonated small molecules that inhibit vascular tube formation. Bioorg Med Chem Lett. 22(13):4467-70. PMCID: PMC3105149. Abstract Tumor-associated angiogenesis is a complex process that involves the interplay among several molecular players such as cell-surface heparan...

Antigen delivery to macrophages using liposomal nanoparticles targeting sialoadhesin/CD169.

2012-06-19

Chen WC, Kawasaki N, Nycholat CM, Han S, Pilotte J, Crocker PR, Paulson JC. (2012) Antigen delivery to macrophages using liposomal nanoparticles targeting sialoadhesin/CD169. PLoS One. 7(6):e39039. PMID: 22723922; PMCID: PMC22723922. Abstract Sialoadhesin (Sn, Siglec-1, CD169) is a member of the sialic acid binding Ig-like lectin (siglec) family...

Specific inactivation of two immunomodulatory SIGLEC genes during human evolution.

2012-06-19

Wang X, Mitra N, Secundino I, Banda K, Cruz P, Padler-Karavani V, Verhagen A, Reid C, Lari M, Rizzi E, Balsamo C, Corti G, De Bellis G, Longo L; NISC Comparative Sequencing Program, Beggs W, Caramelli D, Tishkoff SA, Hayakawa T, Green ED, Mullikin JC,...

Glutathione/thioredoxin systems modulate mitochondrial H2O2 emission: an experimental-computational study.

2012-06-14

Aon MA, Stanley BA, Sivakumaran V, Kembro JM, O'Rourke B, Paolocci N, Cortassa S. (2012) Glutathione/thioredoxin systems modulate mitochondrial H2O2 emission: an experimental-computational study. J Gen Physiol. 139(6):479-91. PMID: 22585969; PMCID: PMC3362521 Abstract The net emission of hydrogen peroxide (H(2)O(2)) from mitochondria results from the balance between...

Death regulates platelet birth and life.

2012-06-14

Hoffmeister KM. (2012) Death regulates platelet birth and life. Blood. 119(24):5617-8. PMID: 22700694 Comment on Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survival. [Blood. 2012] Link to journal: http://www.bloodjournal.org/?sso-checked=true

Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome.

2012-06-10

Rillahan CD, Antonopolous A, Lefort CT, Sonon R, Azadi P, Ley K, Dell A, Haslam SM, Paulson, JC. (2012) Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome. Nat Chem Biol. 8(7):661-8.doi: 10.1038/nchembio.999. PMID: 22683610; PMCID: PMC22683610. Abstract Despite the fundamental roles of sialyl- and fucosyltransferases in...

Prevalence and prognostic value of left ventricular diastolic dysfunction in idiopathic and heritable pulmonary arterial hypertension.

2012-06-06

Tonelli AR, Plana JC, Heresi GA, Dweik RA. (2012) Prevalence and prognostic value of left ventricular diastolic dysfunction in idiopathic and heritable pulmonary arterial hypertension. Chest. 141(6):1457-65. PMID: 2220768. PMCID: PMC3367485   Abstract BACKGROUND: Little is known about the association between left ventricular (LV) diastolic dysfunction and outcomes in patients with idiopathic or...

O-GlcNAcylation of kinases

2012-06-01

Dias WB, Cheung WD, Hart GW. (2012) O-GlcNAcylation of kinases. Biochem Biophys Res Commun. 422(2):224-8. PMID: 22564745; PMCID: PMC3387735 Abstract Recent evidence indicates that site-specific crosstalk between O-GlcNAcylation and phosphorylation and the O-GlcNAcylation of kinases play an important role in regulating cell signaling. However, relatively few kinases...

The origin and function of platelet glycotransferases.

2012-05-21

Wandall HH, Rumjantseva V, Sørensen AL, Patel-Hett S, Josefsson EC, Bennett EP, Italiano JE Jr, Clausen H, Hartwig JH, Hoffmeister KM. (2012) The origin and function of platelet glycotransferases. Bone. 120(3):626-35. PMID: 22613794; PMCID: PMC3401214. Link to journal: http://www.journals.elsevier.com/bone/

The roles of O-linked β-N-acetylglucosamine in cardiovascular physiology and disease.

2012-05-15

Zachara NE. (2012) The roles of O-linked β-N-acetylglucosamine in cardiovascular physiology and disease. Am J Physiol Heart Circ Physiol. 302(10):H1905-18. PMID: 22287582; PMCID: PMC3362101 Abstract More than 1,000 proteins of the nucleus, cytoplasm, and mitochondria are dynamically modified by O-linked β-N-acetylglucosamine (O-GlcNAc), an essential post-translational modification of...

Nitric oxide and asthma severity: towards a better understanding of asthma phenotypes.

2012-05-01

Aytekin M, Dweik RA. (2012) Nitric oxide and asthma severity: towards a better understanding of asthma phenotypes. Clin Exp Allergy. 42(5):614-6. doi: 10.1111/j.1365-2222.2012.03976.x. PMID: 22515385; PMCID: PMC3335741.

Epidermal dendritic cell function is controlled by the hyaluronan microenvironment.

2012-04-26

Muto, J., Carlin, AF, Kim, M., Garica, A., Varki, A., Gallo, R. (2012) Epidermal dendritic cell function is controlled by the hyaluronan microenvironment. J Invest Derm. (In Press) Link to journal: http://www.nature.com/jid/index.html

Multifarious roles of sialic acids in immunity.

2012-04-23

Varki A, Gagneux P. (2012) Multifarious roles of sialic acids in immunity. Ann N Y Acad Sci. 1253:16-36. doi: 10.1111/j.1749-6632.2012.06517.x. PMID: 22524423; PMCID: PMC3357316 Abstract Sialic acids are a diverse family of monosaccharides widely expressed on all cell surfaces of vertebrates and so-called "higher" invertebrates, and on certain...

Tandem mass spectrometry identifies many mouse brain O-GlcNAcylated proteins including EGF domain-specific O-GlcNAc transferase targets.

2012-04-19

Alfaro JF, Gong CX, Monroe ME, Aldrich JT, Clauss TR, Purvine SO, Wang Z, Camp DG 2nd, Shabanowitz J, Stanley P, Hart GW, Hunt DF, Yang F, Smith RD. (2012) Tandem mass spectrometry identifies many mouse brain O-GlcNAcylated proteins including EGF domain-specific O-GlcNAc transferase targets....

Portopulmonary hypertension: a report from the US-based REVEAL Registry.

2012-04-06

Krowka MJ, Miller DP, Barst RJ, Taichman D, Dweik RA, Badesch DB, McGoon MD. (2012) Portopulmonary hypertension: a report from the US-based REVEAL Registry. Chest. 141(4):906-15. PMID: 21778257   Abstract BACKGROUND: We evaluated survival and hospitalization rates in patients with group 1 portopulmonary hypertension (PoPH) in the Registry to Evaluate Early and Long-term Pulmonary Arterial Hypertension...

Sensitive cardiac troponin I predicts poor outcomes in pulmonary arterial hypertension.

2012-04-06

Heresi GA, Tang WH, Aytekin M, Hammel J, Hazen SL, Dweik RA. (2012) Sensitive cardiac troponin I predicts poor outcomes in pulmonary arterial hypertension. Eur Respir J. 39(4):939-44. PMID: 21885398   Abstract Circulating cardiac troponins are markers of myocardial injury. We sought to determine whether cardiac troponin I (cTnI), measured by a sensitive assay, is...

Which anti-platelet therapies might be beneficial in xenotransplantation?

2012-03-26

Schmelzle M, Cowan PJ, Robson SC. (2011) Which anti-platelet therapies might be beneficial in xenotransplantation? Xenotransplantation. 18(2):79-87. doi: 10.1111/j.1399-3089.2011.00628.x. PMID: 21496115; PMCID: PMC3733105 Abstract Xenotransplantation could provide an unlimited and elective supply of grafts, once mechanisms of graft loss and vascular injury are better understood. The development...

Versatile co-expression of graft-protective proteins using 2A-linked cassettes

2012-03-26

Fisicaro N, Londrigan SL, Brady JL, Salvaris E, Nottle MB, O'Connell PJ, Robson SC, d'Apice AJ, Lew AM, Cowan PJ. (2011) Versatile co-expression of graft-protective proteins using 2A-linked cassettes. Xenotransplantation. (2):121-30. doi: 10.1111/j.1399-3089.2011.00631.x. PMID: 21496119. Abstract BACKGROUND: Expression of multiple graft-protective proteins targeted to different locations (i.e., intracellular,...

Abnormal platelet aggregation in idiopathic pulmonary arterial hypertension: role of nitric oxide.

2012-03-15

Aytekin M, Aulak KS, Haserodt S, Chakravarti R, Cody J, Minai OA, Dweik RA. (2012) Abnormal platelet aggregation in idiopathic pulmonary arterial hypertension: role of nitric oxide. Am J Physiol Lung Cell Mol Physiol. 302(6):L512-20. PMID: 22246002; PMCID: PMC3311529. Abstract Idiopathic pulmonary arterial hypertension (IPAH) is a rare...

Anti-inflammatory IgG production requires functional P1 promoter in β-galactoside α2,6-sialyltransferase 1 (ST6Gal-1) gene.

2012-03-15

Jones MB, Nasirikenari M, Lugade AA, Thanavala Y, Lau JT.(2012) Anti-inflammatory IgG production requires functional P1 promoter in β-galactoside α2,6-sialyltransferase 1 (ST6Gal-1) gene. J Biol Chem. 287(19):15365-70. PMID: 22427662; PMCID: PMC3346113. Link to journal: http://www.jbc.org/

B-cell depletion extends the survival of GTKO.hCD46Tg pig heart xenografts in baboons for up to 8 months

2012-03-05

Mohiuddin MM, Corcoran PC, Singh AK, Azimzadeh A, Hoyt RF Jr, Thomas ML, Eckhaus MA, Seavey C, Ayares D, Pierson RN 3rd, Horvath KA. (2012) B-cell depletion extends the survival of GTKO.hCD46Tg pig heart xenografts in baboons for up to 8 months. Am J Transplant. 12(3):763-71....

Electronically Rich N-Substituted Tetrahydroisoquinoline 3-Carboxylic Acid Esters: Concise Synthesis and Conformational Studies.

2012-02-25

Al-Horani RA, Desai UR. (2012) Electronically Rich N-Substituted Tetrahydroisoquinoline 3-Carboxylic Acid Esters: Concise Synthesis and Conformational Studies. Tetrahedron. 68(8):2027-2040. PMID: 22665943. PMCID: PMC3365611. Abstract Recent work in our laboratory has shown that the highly substituted, electronically rich 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid (THIQ3CA) scaffold is a key building block for...

Leukocyte inflammatory responses provoked by pneumococcal sialidase.

2012-02-22

Chang YC, Uchiyama S, Varki A, Nizet V. (2012) Leukocyte inflammatory responses provoked by pneumococcal sialidase. MBio. 3(1). pii: e00220-11. doi: 10.1128/mBio.00220-11. PMID: 22215570; PMCID: PMC3251504 Link to journal: http://mbio.asm.org/

Engineering cellular trafficking via glycosyltransferase-programmed stereosubstitution.

2012-02-21

Sackstein R. (2012) Engineering cellular trafficking via glycosyltransferase-programmed stereosubstitution. Ann N Y Acad Sci. 1253:193-200. doi: 10.1111/j.1749-6632.2011.06421.x. PMID: 22352800; PMCID: PMC3336002. Abstract The proximate hurdle for cell trafficking to any anatomic site is the initial attachment of circulating cells to target tissue endothelium with sufficient strength to overcome...

A synthetic heparan sulfate oligosaccharide library reveals the novel enzymatic action of D-glucosaminyl 3-O-sulfotransferase-3a

2012-02-12

Nguyen TK, Arungundram S, Tran VM, Raman K, Al-Mafraji K, Venot A, Boons GJ, Kuberan B.(2012) A synthetic heparan sulfate oligosaccharide library reveals the novel enzymatic action of D-glucosaminyl 3-O-sulfotransferase-3a. Mol Biosyst. 8(2):609-14.  PMID: 22116385. Abstract Heparan sulfate (HS) glucosaminyl 3-O-sulfotranferases sulfate the C3-hydroxyl group of certain...

The monomer-dimer equilibrium and glycosaminoglycan interactions of chemokine CXCL8 regulate tissue-specific neutrophil recruitment.

2012-02-09

Gangavarapu P, Rajagopalan L, Kolli D, Guerrero-Plata A, Garofalo RP, Rajarathnam K. (2012) The monomer-dimer equilibrium and glycosaminoglycan interactions of chemokine CXCL8 regulate tissue-specific neutrophil recruitment. J Leukoc Biol. 91(2):259-65. PMID: 22140266. PMCID: PMC3290428. Abstract Chemokines exert their function by binding the GPCR class of receptors on...

Polymorphism in the protease-activated receptor-4 gene region associates with platelet activation and perioperative myocardial injury.

2012-02-08

Muehlschlegel JD, Perry TE, Liu KY, Fox AA, Smith SA, Lichtner P, Collard CD, Shernan SK, Hartwig JH, Body SC, Hoffmeister KM. (2012) Polymorphism in the protease-activated receptor-4 gene region associates with platelet activation and perioperative myocardial injury. Am J Hematol. 87(2):161-6. PMID: 22228373 Abstract Protease-activated receptors...

Heart rate recovery predicts clinical worsening in patients with pulmonary arterial hypertension.

2012-02-06

Minai OA, Gudavalli R, Mummadi S, Liu X, McCarthy K, Dweik RA. (2012) Heart rate recovery predicts clinical worsening in patients with pulmonary arterial hypertension. Am J Respir Crit Care Med. 185(4):400-8. PMID: 22108205   Abstract RATIONALE: Reduced heart rate recovery after exercise is associated with increased mortality in cardiopulmonary...

Developmental, malignancy-related, and cross-species analysis of eosinophil, mast cell, and basophil siglec-8 expression.

2012-02-02

Hudson SA, Herrmann H, Du J, Cox P, Haddad el-B, Butler B, Crocker PR, Ackerman SJ, Valent P, Bochner BS. (2011) Developmental, malignancy-related, and cross-species analysis of eosinophil, mast cell, and basophil siglec-8 expression. J Clin Immunol. 31(6):1045-53. PMID: 21938510; PMCID: PMC3329870. Abstract OBJECTIVE: The aim of this study...

Desialylation accelerates platelet clearance after refrigeration and initiates GPIbα metalloproteinase-mediated cleavage in mice.

2012-02-02

Jansen AJ, Josefsson EC, Rumjantseva V, Liu QP, Falet H, Bergmeier W, Cifuni SM, Sackstein R, von Andrian UH, Wagner DD, Hartwig JH, Hoffmeister KM. (2012) Desialylation accelerates platelet clearance after refrigeration and initiates GPIbα metalloproteinase-mediated cleavage in mice. Blood. 119(5):1263-73. PMID: 22101895; PMCID: PMC3277358. Abstract When refrigerated...

IL-33 enhances Siglec-8 mediated apoptosis of human eosinophils.

2012-02-01

Na HJ, Hudson SA, Bochner BS. (2012) IL-33 enhances Siglec-8 mediated apoptosis of human eosinophils. Cytokine. 57(1):169-74. PMID: 22079334; PMCID: PMC3282301. Abstract IL-33 activates eosinophils directly via the ST2 receptor. Like IL-5, IL-33 induces eosinophilia and eosinophilic airway inflammation in mouse models and primes human eosinophil responses. Previously,...

Transgenic over expression of ectonucleotide triphosphate diphosphohydrolase-1 protects against murine myocardial ischemic injury.

2012-02-01

Cai M, Huttinger ZM, He H, Zhang W, Li F, Goodman LA, Wheeler DG, Druhan LJ, Zweier JL, Dwyer KM, He G, d'Apice AJ, Robson SC, Cowan PJ, Gumina RJ. (2011) Transgenic over expression of ectonucleotide triphosphate diphosphohydrolase-1 protects against murine myocardial ischemic injury. J...

Glycomic analysis of human mast cells, eosinophils and basophils.

2012-02-01

North SJ, von Gunten S, Antonopoulos A, Trollope A, MacGlashan DW Jr, Jang-Lee J, Dell A, Metcalfe DD, Kirshenbaum AS, Bochner BS, Haslam SM. (2012) Glycomic analysis of human mast cells, eosinophils and basophils. Glycobiology. 22(1):12-22. PMID: 21725073; PMCID: PMC3230278. Abstract In allergic diseases such as asthma, eosinophils,...

ADAMTSL4, a secreted glycoprotein widely distributed in the eye, binds fibrillin-1 microfibrils and accelerates microfibril biogenesis.

2012-01-31

Gabriel LA, Wang LW, Bader H, Ho JC, Majors AK, Hollyfield JG, Traboulsi EI, Apte SS. (2012) ADAMTSL4, a secreted glycoprotein widely distributed in the eye, binds fibrillin-1 microfibrils and accelerates microfibril biogenesis. Invest Ophthalmol Vis Sci. 53(1):461-9. PMID: 21989719; PMCID: PMC3292378. Abstract PURPOSE: ADAMTSL4 mutations cause autosomal recessive...

Sulfated, low molecular weight lignins inhibit a select group of heparin-binding serine proteases.

2012-01-06

Henry BL, Thakkar JN, Liang A, Desai UR. (2012) Sulfated, low molecular weight lignins inhibit a select group of heparin-binding serine proteases. Biochem Biophys Res Commun. 417(1):382-6. PMID: 22155248; PMCID: PMC3259278. Abstract Sulfated low molecular weight lignins (LMWLs), designed as oligomeric mimetics of low molecular weight heparins (LMWHs),...

Enhanced inflammation and accelerated wound closure following tetraphorbol ester application or full-thickness wounding in mice lacking hyaluronan synthases Has1 and Has3.

2012-01-05

Mack JA, Feldman RJ, Itano N, Kimata K, Lauer M, Hascall VC, Maytin EV. (2012) Enhanced inflammation and accelerated wound closure following tetraphorbol ester application or full-thickness wounding in mice lacking hyaluronan synthases Has1 and Has3. J Invest Dermatol. 132(1):198-207. doi: 10.1038/jid.2011.248. PMID: 21850020; PMCID: PMC3360468. Abstract Hyaluronan...

An official ATS clinical practice guideline: interpretation of exhaled nitric oxide levels (FENO) for clinical applications.

2012-01-02

Dweik RA, Boggs PB, Erzurum SC, Irvin CG, Leigh MW, Lundberg JO, Olin AC, Plummer AL, Taylor DR; American Thoracic Society Committee on Interpretation of Exhaled Nitric Oxide Levels (FENO) for Clinical Applications. (2011) An official ATS clinical practice guideline: interpretation of exhaled nitric oxide levels (FENO) for clinical applications. Am J Respir Crit Care Med. 184(5):602-15....

Human Siglec-5 inhibitory receptor and immunoglobulin A (IgA) have separate binding sites in streptococcal beta protein.

2012-01-01

Nordström T, Movert E, Olin AI, Ali SR, Nizet V, Varki A, Areschoug T. (2011) Human Siglec-5 inhibitory receptor and immunoglobulin A (IgA) have separate binding sites in streptococcal beta protein. J Biol Chem. 286:33981-91. PMID: 21795693. PMCID: PMC3190825 Abstract Sialic acid-binding immunoglobulin-like lectins (Siglecs) are receptors...

The great challenge for exhaled breath analysis: embracing complexity, delivering simplicity.

2012-01-01

Dweik RA. (2011) The great challenge for exhaled breath analysis: embracing complexity, delivering simplicity. J Breath Res. 5(3):030201.  PMID: 21900732. Link to journal: http://iopscience.iop.org/1752-7163/

Pericellular versican regulates the fibroblast-myofibroblast transition: a role for ADAMTS5 protease-mediated proteolysis.

2012-01-01

Hattori N, Carrino DA, Lauer ME, Vasanji A, Wylie JD, Nelson CM, Apte SS. (2011) Pericellular versican regulates the fibroblast-myofibroblast transition: a role for ADAMTS5 protease-mediated proteolysis. J Biol Chem. 286(39):34298-310. PMID: 21828051; PMCID: PMC3190794. Abstract The cell and its glycosaminoglycan-rich pericellular matrix (PCM) comprise a functional unit....

Is N-sulfation just a gateway modification during heparan sulfate biosynthesis?

2012-01-01

Raman K, Nguyen TK, Kuberan B. (2011) Is N-sulfation just a gateway modification during heparan sulfate biosynthesis? FEBS Lett. 585(21):3420-3. PMID: 21983289. Abstract Several biologically important growth factor-heparan sulfate (HS) interactions are regulated by HS sulfation patterns. However, the biogenesis of these combinatorial sulfation patterns is largely unknown....

Glycosaminoglycan binding facilitates entry of a bacterial pathogen into central nervous systems.

2012-01-01

Chang YC, Wang Z, Flax LA, Xu D, Esko JD, Nizet V, Baron MJ. (2011) Glycosaminoglycan binding facilitates entry of a bacterial pathogen into central nervous systems. PLoS Pathog. 7(6):e1002082. PMID: 21731486. PMCID: PMC3121876 Abstract Certain microbes invade brain microvascular endothelial cells (BMECs) to breach the blood-brain...

CD44 and HCELL: preventing hematogenous metastasis at step 1.

2012-01-01

Jacobs PP, Sackstein R. (2011) CD44 and HCELL: preventing hematogenous metastasis at step 1. FEBS Lett. 585(20):3148-58. PMID: 21827751; PMCID: PMC3195965. Abstract Despite great strides in our knowledge of the genetic and epigenetic changes underlying malignancy, we have limited information on the molecular basis of metastasis. Over 90%...

Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

2012-01-01

Zachara NE, Vosseller K, Hart GW. (2011) Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Protein Sci. Chapter 12:Unit12.8. PMID: 22045558; PMCID: PMC3349994 Abstract O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type...

Understanding glycomechanics using mathematical modeling: a review of current approaches to simulate cellular glycosylation reaction networks.

2012-01-01

Puri A, Neelamegham S. (2012) Understanding glycomechanics using mathematical modeling: a review of current approaches to simulate cellular glycosylation reaction networks. Ann Biomed Eng. (4):816-27. PMID: 22090146; PMCID: PMC3311736. Abstract Following the footsteps of genomics and proteomics, recent years have witnessed the growth of large-scale experimental methods in...

Heparan sulfate regulates VEGF165- and VEGF121-mediated vascular hyperpermeability.

2012-01-01

Xu D, Fuster MM, Lawrence R, Esko JD. (2011) Heparan sulfate regulates VEGF165- and VEGF121-mediated vascular hyperpermeability. J Biol Chem. 286:737-45. PMID: 20974861. PMCID: PMC3013032. Abstract VEGF was first described as vascular permeability factor, a potent inducer of vascular leakage. Genetic evidence indicates that VEGF-stimulated endothelial proliferation...

Glycoengineering of HCELL, the human bone marrow homing receptor: sweetly programming cell migration.

2012-01-01

Sackstein R. (2012) Glycoengineering of HCELL, the human bone marrow homing receptor: sweetly programming cell migration. Ann Biomed Eng. 40(4):766-76. PMID: 22068886; PMCID: PMC3311703. Abstract The successful clinical implementation of adoptive cell therapeutics, including bone marrow transplantation and other stem cell-based treatments, depends critically on the ability to...

Heparan sulfate is essential for high mobility group protein 1 (HMGB1) signaling by the receptor for advanced glycation end products (RAGE).

2012-01-01

Xu D, Young J, Song D, Esko JD. (2011) Heparan sulfate is essential for high mobility group protein 1 (HMGB1) signaling by the receptor for advanced glycation end products (RAGE). J Biol Chem. 286:41736-44. PMID: 21990362. PMCID: PMC3308882. Abstract In a proteomic search for heparan sulfate-binding proteins...

Role of receptor for hyaluronic acid-mediated motility (RHAMM) in low molecular weight hyaluronan (LMWHA)-mediated fibrosarcoma cell adhesion.

2012-01-01

Kouvidi K, Berdiaki A, Nikitovic D, Katonis P, Afratis N, Hascall VC, Karamanos NK, Tzanakakis GN. (2011) Role of receptor for hyaluronic acid-mediated motility (RHAMM) in low molecular weight hyaluronan (LMWHA)-mediated fibrosarcoma cell adhesion. J Biol Chem. 286:38509-20. PMID: 21914806. PMCID: PMC3207394. Abstract Hyaluronan (HA) modulates key...

The inducible kinase IKKi is required for IL-17-dependent signaling associated with neutrophilia and pulmonary inflammation.

2012-01-01

Bulek K, Liu C, Swaidani S, Wang L, Page RC, Gulen MF, Herjan T, Abbadi A, Qian W, Sun D, Lauer M, Hascall V, Misra S, Chance MR, Aronica M, Hamilton T, Li X. (2011) The inducible kinase IKKi is required for IL-17-dependent signaling associated...

Glycosaminoglycans and glucose prevent apoptosis in 4-methylumbelliferone-treated human aortic smooth muscle cells.

2012-01-01

Vigetti D, Rizzi M, Moretto P, Deleonibus S, Dreyfuss JM, Karousou E, Viola M, Clerici M, Hascall VC, Ramoni MF, De Luca G, Passi A. (2011) Glycosaminoglycans and glucose prevent apoptosis in 4-methylumbelliferone-treated human aortic smooth muscle cells. J Biol Chem. 286:34497-503. PMID: 21768115. PMCID:...

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Exogenous addition of a C-xylopyranoside derivative stimulates keratinocyte dermatan sulfate synthesis and promotes migration.

2012-01-01

Muto J, Naidu NN, Yamasaki K, Pineau N, Breton L, Gallo RL. (2011) Exogenous addition of a C-xylopyranoside derivative stimulates keratinocyte dermatan sulfate synthesis and promotes migration. PLoS One. 6(10):e25480. PMID: 21998662. PMCID: PMC3187761.   Abstract As C-Xyloside has been suggested to be an initiator of glycosaminoglycan (GAG)...

Strengths and limitations of the six-minute-walk test: a model biomarker study in idiopathic pulmonary fibrosis.

2011-05-06

Heresi GA, Dweik RA. (2011) Strengths and limitations of the six-minute-walk test: a model biomarker study in idiopathic pulmonary fibrosis. Am J Respir Crit Care Med. 183(9):1122-4. PMID: 21531951 Link to journal: http://www.atsjournals.org/journal/ajrccm

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