Publications

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

McBride R, Paulson JC, de Vries RP. J Vis Exp. 2016 May 29;(111). doi: 10.3791/53847. PMID: 27284789 Abstract Influenza A virus (IAV) hemagglutinins recognize sialic acids on the cell surface as functional receptors to gain entry into cells. Wild waterfowl are the … Continued

Gangliosides of the Vertebrate Nervous System

Schnaar RL. J Mol Biol. 2016 Aug 14;428(16):3325-36. doi: 10.1016/j.jmb.2016.05.020. Review. PMID: 27261254 Abstract Gangliosides, sialylated glycosphingolipids, found on all vertebrate cells and tissues, are major molecular determinants on the surfaces of vertebrate nerve cells. Composed of a sialylated glycan attached to … Continued

Mucins and Their Sugars. Critical Mediators of Hyperreactivity and Inflammation

Raclawska DS, Ttofali F, Fletcher AA, Harper DN, Bochner BS, Janssen WJ, Evans CM. Ann Am Thorac Soc. 2016 Mar;13 Suppl 1:S98-9. doi: 10.1513/AnnalsATS.201511-743MG. PMID: 27027967 Abstract Excessive mucus causes severe airflow obstruction in fatal asthma. It is also present in mild … Continued

Glycobiology simplified: diverse roles of glycan recognition in inflammation

Schnaar RL. J Leukoc Biol. 2016 Jun;99(6):825-38. doi: 10.1189/jlb.3RI0116-021R. Review. PMID: 27004978 Abstract Glycans and complementary glycan-binding proteins are essential components in the language of cell-cell interactions in immunity. The study of glycan function is the purview of glycobiology, which has often … Continued

“Siglec”ting the allergic response for therapeutic targeting

Bochner BS. Glycobiology. 2016 Jun;26(6):546-52. doi: 10.1093/glycob/cww024. Review. PMID: 26911285 Abstract As a physician-scientist, I have pursued research related to translational immunology with the goal of improving our ability to diagnose and treat allergic, immunologic and other diseases involving eosinophils, basophils and … Continued

Regulation of airway inflammation by Siglec-8 and Siglec-9 sialoglycan ligand expression

Schleimer RP, Schnaar RL, Bochner BS. Curr Opin Allergy Clin Immunol. 2016 Feb;16(1):24-30. doi: 10.1097/ACI.0000000000000234. Review. PMID: 26694037 Abstract PURPOSE OF REVIEW: Inflammatory cells involved in the allergic response, including eosinophils, mast cells, basophils, and neutrophils, express sialoglycan-binding proteins such as Siglec-8 … Continued

Therapeutic Targeting of Siglecs using Antibody- and Glycan-Based Approaches

Angata T, Nycholat CM, Macauley MS. Trends Pharmacol Sci. 2015 Oct;36(10):645-60. doi: 10.1016/j.tips.2015.06.008. Review. PMID: 26435210 Abstract The sialic acid-binding immunoglobulin-like lectins (Siglecs) are a family of immunomodulatory receptors whose functions are regulated by their glycan ligands. Siglecs are attractive therapeutic targets … Continued

Mouse Siglec-1 Mediates trans-Infection of Surface-bound Murine Leukemia Virus in a Sialic Acid N-Acyl Side Chain-dependent Manner

Erikson E, Wratil PR, Frank M, Ambiel I, Pahnke K, Pino M, Azadi P, Izquierdo-Useros N, Martinez-Picado J, Meier C, Schnaar RL, Crocker PR, Reutter W, Keppler OT. J Biol Chem. 2015 Nov 6;290(45):27345-59. doi: 10.1074/jbc.M115.681338. PMID: 26370074 Abstract Siglec-1 (sialoadhesin, CD169) … Continued

Novel Therapies for Eosinophilic Disorders

Bochner BS.Immunol Allergy Clin North Am. 2015 Aug;35(3):577-98. doi: 10.1016/j.iac.2015.05.007. Review. PMID: 26209901 Abstract Current therapies for eosinophilic disorders are limited. Most treatment approaches remain empirical, are not supported by data from controlled clinical trials, involve the off-label use of agents … Continued

B7-H3 Promotes Pathogenesis of Autoimmune Disease and Inflammation by Regulating the Activity of Different T Cell Subsets

Luo L, Zhu G, Xu H, Yao S, Zhou G, Zhu Y, Tamada K, Huang L, Flies AD, Broadwater M, Ruff W, van Deursen JM, Melero I, Zhu Z, Chen L. PLoS One. 2015 Jun 11;10(6):e0130126. doi: 10.1371/journal.pone.0130126. PMID: 26065426 Abstract B7-H3 … Continued

Efficient Mapping of Sulfated Glycotopes by Negative Ion Mode nanoLC-MS/MS-Based Sulfoglycomic Analysis of Permethylated Glycans

Cheng CW, Chou CC, Hsieh HW, Tu Z, Lin CH, Nycholat C, Fukuda M, Khoo KH. Anal Chem. 2015 Jun 16;87(12):6380-8. doi: 10.1021/acs.analchem.5b01409. PMID: 26016788 Abstract We have previously developed the enabling techniques for sulfoglycomics based on mass spectrometry (MS) analysis of … Continued

The Role of Vitamin D in Pediatric Asthma

Bantz SK, Zhu Z, Zheng T. Ann Pediatr Child Health. 2015;3(1). pii: 1032. PMID: 25938135 Abstract The detrimental effects of vitamin D deficiency in pediatrics have become increasingly apparent and extend beyond skeletal health. Unfortunately, vitamin D deficiency is highly prevalent in … Continued

Reply: To PMID 25497369

Bochner BS, Kiwamoto T, Katoh T, Zhu Z, Tiemeyer M. J Allergy Clin Immunol. 2015 Jun;135(6):1662-3. doi: 10.1016/j.jaci.2015.02.028. No abstract available. PMID: 25863979 Abstract

Endogenous airway mucins carry glycans that bind Siglec-F and induce eosinophil apoptosis

Kiwamoto T, Katoh T, Evans CM, Janssen WJ, Brummet ME, Hudson SA, Zhu Z, Tiemeyer M, Bochner BS. J Allergy Clin Immunol. 2015 May;135(5):1329-40.e1-9. doi: 10.1016/j.jaci.2014.10.027. PMID: 25497369 Abstract BACKGROUND: Sialic acid-binding, immunoglobulin-like lectin (Siglec) F is a glycan-binding protein selectively expressed … Continued

Systemic blockade of sialylation in mice with a global inhibitor of sialyltransferases

Macauley MS, Arlian BM, Rillahan CD, Pang PC, Bortell N, Marcondes MC, Haslam SM, Dell A, Paulson JC. J Biol Chem. 2014 Dec 19;289(51):35149-58. doi: 10.1074/jbc.M114.606517. PMID: 25368325 Abstract Sialic acid terminates glycans of glycoproteins and glycolipids that play numerous biological roles … Continued

Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation

Fang P, Zhou L, Zhou Y, Kolls JK, Zheng T, Zhu Z. PLoS One. 2014 Sep 25;9(9):e107454. doi: 10.1371/journal.pone.0107454. PMID: 25254361 Abstract IL-22 is a Th17/Th22 cytokine that is increased in asthma. However, recent animal studies showed controversial findings in the effects … Continued

Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing

Fang P, Zhou L, Zhou Y, Kolls JK, Zheng T, Zhu Z. PLoS One. 2014 Sep 25;9(9):e107454. doi: 10.1371/journal.pone.0107454. PMID: 25254361 Abstract IL-22 is a Th17/Th22 cytokine that is increased in asthma. However, recent animal studies showed controversial findings in the effects … Continued

TRPA1-dependent pruritus in IL-13-induced chronic atopic dermatitis

Oh MH, Oh SY, Lu J, Lou H, Myers AC, Zhu Z, Zheng T. J Immunol. 2013 Dec 1;191(11):5371-82. doi: 10.4049/jimmunol.1300300. PMID: 24140646 Abstract Chronic debilitating pruritus is a cardinal feature of atopic dermatitis (AD). Little is known about the underlying mechanisms. … Continued

Glycans and glycan-binding proteins in immune regulation: A concise introduction to glycobiology for the allergist.

Schnaar RL. (2015) Glycans and glycan-binding proteins in immune regulation: A concise introduction to glycobiology for the allergist. J Allergy Clin Immunol. 2015 Jan 30. pii: S0091-6749(15)00007-X. doi: 10.1016/j.jaci.2014.10.057. [Epub ahead of print] PMID: 25649080. Abstract Cells are endowed with … Continued

Role of siglecs and related glycan-binding proteins in immune responses and immunoregulation.

Bochner BS, Zimmermann N. (2015) Role of siglecs and related glycan-binding proteins in immune responses and immunoregulation. J Allergy Clin Immunol. 2015 Jan 12. pii: S0091-6749(14)01734-5. doi: 10.1016/j.jaci.2014.11.031. [Epub ahead of print] PMID: 25592986. Abstract Virtually all cells and extracellular … Continued

Improved in-gel reductive Beta-elimination for comprehensive O-linked and sulfo-glycomics by mass spectrometry

Nix DB, Kumagai T, Katoh T, Tiemeyer M, Aoki K. (2014) Improved in-gel reductive β-elimination for comprehensive O-linked and sulfo-glycomics by mass spectrometry. J Vis Exp.(93):e51840. doi: 10.3791/51840. PMID: 25489664. Abstract Separation of proteins by SDS-PAGE followed by in-gel proteolytic … Continued

Global microRNA expression is essential for murine mast cell development in vivo.

Oh SY, Brandal S, Kapur R, Zhu Z, Takemoto CM. (2014) Global microRNA expression is essential for murine mast cell development in vivo. Exp Hematol. 42(10):919-23. PMID: 25201754. PMCID: PMC4250304. Abstract MicroRNAs (miRNAs) are small, noncoding RNAs that have been … Continued

Siglec-mediated regulation of immune cell function in disease

Macauley MS, Crocker PR, Paulson JC. (2014) Siglec-mediated regulation of immune cell function in disease. Nat Rev Immunol. 14(10):653-66. PMID: 25234143. PMCID: PMC4191907. Abstract All mammalian cells display a diverse array of glycan structures that differ from those that are … Continued

Targeted delivery of mycobacterial antigens to human dendritic cells via Siglec-7 induces robust T cell activation.

Kawasaki N, Rillahan CD, Cheng TY, Van Rhijn I, Macauley MS, Moody DB, Paulson JC. (2014) Targeted delivery of mycobacterial antigens to human dendritic cells via Siglec-7 induces robust T cell activation. J Immunol. 193(4):1560-6. doi: 10.4049/jimmunol.1303278. PMID: 25000981. PMCID: … Continued

Regulation of nasal airway homeostasis and inflammation in mice by SHP-1 and Th2/Th1 signaling pathways.

Cho SH, Oh SY, Lane AP, Lee J, Oh MH, Lee S, Zheng T, Zhu Z. (2014) Regulation of nasal airway homeostasis and inflammation in mice by SHP-1 and Th2/Th1 signaling pathways. PLoS One. 9(8):e103685. doi: 10.1371/journal.pone.0103685. PMID: 25090641. PMCID: PMC4121172. Abstract Allergic rhinitis is a chronic … Continued

Microbial glycan microarrays define key features of host-microbial interactions.

Stowell SR, Arthur CM, McBride R, Berger O, Razi N, Heimburg-Molinaro J, Rodrigues LC, Gourdine JP, Noll AJ, von Gunten S, Smith DF, Knirel YA, Paulson JC, Cummings RD. (2014) Microbial glycan microarrays define key features of host-microbial interactions. Nat … Continued

Disubstituted Sialic Acid Ligands Targeting Siglecs CD33 and CD22 Associated with Myeloid Leukaemias and B Cell Lymphomas.

Rillahan CD, Macauley MS, Schwartz E, He Y, McBride R, Arlian BM, Rangarajan J, Fokin VV, Paulson JC. (2014) Disubstituted Sialic Acid Ligands Targeting Siglecs CD33 and CD22 Associated with Myeloid Leukaemias and B Cell Lymphomas. Chem Sci. 5(6):2398-2406. PMID: 24921038; PMCID: PMC4048721 Abstract The siglec family of sialic acid-binding proteins are endocytic immune … Continued

Mechanisms of Siglec-F-induced eosinophil apoptosis: a role for caspases but not for SHP-1, Src kinases, NADPH oxidase or reactive oxygen.

Mao H, Kano G, Hudson SA, Brummet M, Zimmermann N, Zhu Z, Bochner BS. (2013) Mechanisms of Siglec-F-induced eosinophil apoptosis: a role for caspases but not for SHP-1, Src kinases, NADPH oxidase or reactive oxygen. PLoS One. 8(6):e68143. PMID: 23840825; PMCID: PMC3695997 Abstract BACKGROUND: Siglec-F and Siglec-8 are functional paralog proapoptotic cell … Continued

In-gel β-elimination and aqueous-organic partition for improved O- and sulfoglycomics.

Kumagai T, Katoh T, Nix DB, Tiemeyer M, Aoki K. (2013) In-gel β-elimination and aqueous-organic partition for improved O- and sulfoglycomics. Anal Chem. 85(18):8692-9. PMID: 23937624 Abstract Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique for protein separation, and in-gel tryptic digestion of resolved protein bands … Continued

Synthesis of Biologically Active N- and O-Linked Glycans with Multisialylated Poly-N-acetyllactosamine Extensions Using P. damsela α2-6 Sialyltransferase.

Nycholat CM, Peng W, McBride R, Antonopoulos A, de Vries RP, Polonskaya Z, Finn MG, Dell A, Haslam SM, Paulson JC. Synthesis of Biologically Active N- and O-Linked Glycans with Multisialylated Poly-N-acetyllactosamine Extensions Using P. damsela α2-6 Sialyltransferase. J Am Chem Soc. 135(49):18280-3. PMID: 24256304 PMCID: PMC3901641.   Abstract Sialosides on N- and O-linked … Continued

Deficiency of gp91phox inhibits allergic airway inflammation.

Sevin CM, Newcomb DC, Toki S, Han W, Sherrill TP, Boswell MG, Zhu Z, Collins RD, Boyd KL, Goleniewska K, Huckabee MM, Blackwell TS, Peebles RS Jr. (2013) Deficiency of gp91phox inhibits allergic airway inflammation. Am J Respir Cell Mol Biol. 49(3):396-402. PMID: 23590311 Abstract Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, a multienzyme complex, is the major … Continued

Mice deficient in the St3gal3 gene product α2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation.

Kiwamoto T, Brummet ME, Wu F, Motari MG, Smith DF, Schnaar RL, Zhu Z, Bochner BS. (2013) Mice deficient in the St3gal3 gene product α2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation. J Allergy Clin Immunol. 133(1):240-7.e1-3. PMID: 23830412 PMCID: PMC3874253.   Abstract BACKGROUND: Sialic acid-binding immunoglobulin-like lectin (Siglec)-F is … Continued

The role of lung epithelial ligands for Siglec-8 and Siglec-F in eosionophilic inflammation.

Kiwamoto, T., Katoh, T., Tiemeyer, M., Bochner,BS. (2013) The role of lung epithelial ligands for Siglec-8 and Siglec-F in eosionophilic inflammation. Curr Opin Allergy Clin Immunol. 13(1):106-11. PMID: 23160308 Abstract PURPOSE OF REVIEW: Siglec-8 and Siglec-F are single pass transmembrane inhibitory receptors found … Continued

Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation.

Kawasaki N, Vela JL, Nycholat CM, Rademacher C, Khurana A, van Rooijen N, Crocker PR, Kronenberg M, Paulson JC. (2013) Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation. Proc Natl Acad Sci U S A. 110(19):7826-31. PMID: 23610394; PMCID: PMC3651435 … Continued

On-Chip Synthesis and Screening of a Sialoside Library Yields a High Affinity Ligand for Siglec-7.

Rillahan CD, Schwartz E, Rademacher C, McBride R, Rangarajan J, Fokin VV, Paulson JC. (2013) On-Chip Synthesis and Screening of a Sialoside Library Yields a High Affinity Ligand for Siglec-7. ACS Chem Biol. PMID: 23597400; PMCID: PMC3751994 Abstract The Siglec family of sialic acid-binding proteins are differentially expressed on white blood … Continued

Epicutaneous exposure to staphylococcal superantigen enterotoxin B enhances allergic lung inflammation via an IL-17A dependent mechanism.

Yu J, Oh MH, Park JU, Myers AC, Dong C, Zhu Z, Zheng T. (2012) Epicutaneous exposure to staphylococcal superantigen enterotoxin B enhances allergic lung inflammation via an IL-17A dependent mechanism. PLoS One. 7(7):e39032. PMID: 22848348; PMCID: PMC3407176 Abstract Atopic dermatitis (AD) is the initial step of the atopic march: the progression … Continued

SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.

Zhou L, Oh SY, Zhou Y, Yuan B, Wu F, Oh MH, Wang Y, Takemoto C, Van Rooijen N, Zheng T, Zhu Z. (2013) SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis. PLoS One. 8(2):e55763. PMID: 23390550; PMCID: PMC3563592   Abstract Allergic inflammation and severe allergic reactions (anaphylaxis) are important … Continued

Workshop report from the National Institutes of Health Taskforce on the Research Needs of Eosinophil-Associated Diseases (TREAD).

Bochner BS, Book W, Busse WW, Butterfield J, Furuta GT, Gleich GJ, Klion AD, Lee JJ, Leiferman KM, Minnicozzi M, Moqbel R, Rothenberg ME, Schwartz LB,Simon HU, Wechsler ME, Weller PF. (2012) Workshop report from the National Institutes of Health Taskforce on the Research Needs of Eosinophil-Associated Diseases (TREAD). J Allergy Clin Immunol. 130(3):587-96. PMID: 22935587. PMCID: PMC3432981   Abstract BACKGROUND: … Continued

Click and pick: identification of sialoside analogues for siglec-based cell targeting.

Rillahan CD, Schwartz E, McBride R, Fokin VV, Paulson JC. (2012) Click and pick: identification of sialoside analogues for siglec-based cell targeting. Angew Chem Int Ed Engl. 51(44):11014-8. doi: 10.1002/anie.201205831. PMID: 23038623. PMCID: PMC3480989 Abstract Click ‘n’ chips: Azide and alkyne-bearing sialic … Continued

Biomarkers of eosinophil involvement in allergic and eosinophilic diseases: review of phenotypic and serum markers including a novel assay to quantify levels of soluble Siglec-8.

Na HJ, Hamilton RG, Klion AD, Bochner BS. (2012) Biomarkers of eosinophil involvement in allergic and eosinophilic diseases: review of phenotypic and serum markers including a novel assay to quantify levels of soluble Siglec-8. J Immunol Methods. 383:39-46. PMID: 22683541. … Continued

In silico-aided design of a glycan ligand of sialoadhesin for in vivo targeting of macrophages.

Nycholat CM, Rademacher C, Kawasaki N, Paulson JC. (2012) In silico-aided design of a glycan ligand of sialoadhesin for in vivo targeting of macrophages. J Am Chem Soc. 134:15696-9. PMID: 22967315 PMCID: PMC3462166. Abstract Cell-specific delivery of therapeutic agents using … Continued

Siglec-8 as a drugable target to treat eosinophil and mast cell-associated conditions.

Kiwamoto T, Kawasaki N, Paulson JC, Bochner BS. (2012) Siglec-8 as a drugable target to treat eosinophil and mast cell-associated conditions. Pharmacol Ther. 135:327-36. PMID: 22749793. Abstract Siglecs (sialic acid immunoglobulin-like lectins) are members of the immunoglobulin gene family that … Continued

Antigen delivery to macrophages using liposomal nanoparticles targeting sialoadhesin/CD169.

Chen WC, Kawasaki N, Nycholat CM, Han S, Pilotte J, Crocker PR, Paulson JC. (2012) Antigen delivery to macrophages using liposomal nanoparticles targeting sialoadhesin/CD169. PLoS One. 7(6):e39039. PMID: 22723922; PMCID: PMC22723922. Abstract Sialoadhesin (Sn, Siglec-1, CD169) is a member of the … Continued

Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome.

Rillahan CD, Antonopolous A, Lefort CT, Sonon R, Azadi P, Ley K, Dell A, Haslam SM, Paulson, JC. (2012) Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome. Nat Chem Biol. 8(7):661-8.doi: 10.1038/nchembio.999. PMID: 22683610; PMCID: PMC22683610. Abstract Despite the … Continued

Developmental, malignancy-related, and cross-species analysis of eosinophil, mast cell, and basophil siglec-8 expression.

Hudson SA, Herrmann H, Du J, Cox P, Haddad el-B, Butler B, Crocker PR, Ackerman SJ, Valent P, Bochner BS. (2011) Developmental, malignancy-related, and cross-species analysis of eosinophil, mast cell, and basophil siglec-8 expression. J Clin Immunol. 31(6):1045-53. PMID: 21938510; PMCID: … Continued

IL-33 enhances Siglec-8 mediated apoptosis of human eosinophils.

Na HJ, Hudson SA, Bochner BS. (2012) IL-33 enhances Siglec-8 mediated apoptosis of human eosinophils. Cytokine. 57(1):169-74. PMID: 22079334; PMCID: PMC3282301. Abstract IL-33 activates eosinophils directly via the ST2 receptor. Like IL-5, IL-33 induces eosinophilia and eosinophilic airway inflammation in mouse … Continued

Glycomic analysis of human mast cells, eosinophils and basophils.

North SJ, von Gunten S, Antonopoulos A, Trollope A, MacGlashan DW Jr, Jang-Lee J, Dell A, Metcalfe DD, Kirshenbaum AS, Bochner BS, Haslam SM. (2012) Glycomic analysis of human mast cells, eosinophils and basophils. Glycobiology. 22(1):12-22. PMID: 21725073; PMCID: PMC3230278. Abstract … Continued