Click and pick: identification of sialoside analogues for siglec-based cell targeting.

Rillahan CD, Schwartz E, McBride R, Fokin VV, Paulson JC. (2012) Click and pick: identification of sialoside analogues for siglec-based cell targeting. Angew Chem Int Ed Engl. 51(44):11014-8. doi: 10.1002/anie.201205831. PMID: 23038623. PMCID: PMC3480989

Abstract

Click ‘n’ chips: Azide and alkyne-bearing sialic acids (purple diamond; see picture) were subjected to high-throughput click chemistry to generate a library of sialic acid analogues. Microarray printing of the library and screening with the siglec family of sialic-acid-binding proteins, led to the identification of high-affinity ligands for siglec-9 and siglec-10.