Studying glycosaminoglycan-protein interactions using capillary electrophoresis

Liang A1, Desai UR. Studying glycosaminoglycan-protein interactions using capillary electrophoresis. Methods Mol Biol. 2015;1229:355-75. doi: 10.1007/978-1-4939-1714-3_28. PMID: 25325965

Methods for studying interactions between glycosaminoglycans (GAGs) and proteins have assumed considerable significance as their biological importance increases. Capillary electrophoresis (CE) is a powerful method to study these interactions due to its speed, high efficiency, and low sample/reagent consumption. In addition, CE works effectively under a wide range of physiologically relevant conditions. This chapter presents state-of-the-art on CE methods for studying GAG-protein interactions including affinity capillary electrophoresis (ACE), capillary zone electrophoresis (CZE), frontal analysis (FA)/frontal analysis continuous capillary electrophoresis (FACCE), and capillary electrokinetic chromatography (CEC) with detailed experimental protocols for ACE and CZE methods.