Shared Resources

Link to HSPG-PEG Website

Synthetic Resource

Reagents available include: a) Computationally designed heparan sulfates (HS) (μg levels); b) A library of synthetic xylosides for in vitro synthesis of glycoaminoglycans (GAGs).

Reagents within 1 year include: a) Expanded library of HS molecules; b) Additional xylosides

Costs: Agreement of collaboration and/or charge for costs involved in synthesis

Availability: Immediate and Upcoming

Personnel


Computational Resources

Technologies available include: a) Computational screening algorithm for designing GAG structures with potential therapeutic benefits; b) Ready-made virtual library of 46,586 natural HS hexasaccharide structures; c) Software licenses for operating computational screening algorithm
Technology within 1 year include: a) A larger library of HS octasaccharide and decasaccharide structures; b) Algorithm for screening of longer HSs

Costs: Initial studies will be collaborative. Advanced studies will involved service, user and technician fees

Availability: Immediate and Upcoming

Personnel


GAG Analysis Resources

Resources available include: a) nESI-qTOF-MS spectrometer for structural analysis of GAGs; b) Capillary electrophoresis for studying how GAGs interact with proteins of physiological or pharmacological relevance; c) UPLC – TDQ MS spectrometer for structural analysis of GAGs and their complexes with proteins

Costs: Setup, user and service fees

Availability: Immediate

Personnel


Biophysics Resources

Technologies available include: a) Applied Photophysics stopped-flow fluorimeter for the study of kinetics of GAG interacting with proteins; b) Atomic Force Microscopy for study of interactions of GAGs at a single molecule level; c) High resolution NMR for solution structures of GAG – protein complexes; d) Surface Plasmon Resonance for measuring affinities of GAG for proteins; e) Isothermal calorimetry for thermodynamics of GAG binding to proteins; f) analytical ultracentrifugation for stoichiometry of GAG – protein complexes

Technology within 1 year include: a) HS Array Chips for rapid identification of structures of interest from HS library

Costs: Setup, user and service fees

Availability: Immediate and Upcoming

Personnel


Chemokine Resources

Reagents available include: a) Human interleukin – 8; b) Human neutrophil activating peptide – 8; c) Mouse KC
Reagents within 1 year include: a) 13C and 15N – labeled chemokines for NMR structural studies; b) monomer and dimer variants of chemokines for biophysical, structural, and functional studies; c) chemokine mutants – for structural and functional studies

Costs:Charge for supplying expressed proteins

Availability: Immediate and Upcoming

Personnel


Coagulation Function Resources

Technology available include: a) In vitro assays for testing effect on coagulation dysfunction; b) Ex vivo model of organ hemoperfusion; c) In vivo nonhuman primate model of organ transplantation.

Costs: Charge or collaborative arrangement for evaluation in these models

Availability: Immediate

Personnel