Is N-sulfation just a gateway modification during heparan sulfate biosynthesis?

Raman K, Nguyen TK, Kuberan B. (2011) Is N-sulfation just a gateway modification during heparan sulfate biosynthesis? FEBS Lett. 585(21):3420-3. PMID: 21983289.

Abstract

Several biologically important growth factor-heparan sulfate (HS) interactions are regulated by HS sulfation patterns. However, the biogenesis of these combinatorial sulfation patterns is largely unknown. N-Deacetylase/N-sulfotrasferase (NDST) converts N-acetyl-d-glucosamine residues to N-sulfo-d-glucosamine residues. This enzyme is suggested to be a gateway enzyme because N-sulfation dictates the final HS sulfation pattern. It is known that O-sulfation blocks C5-epimerase, which acts immediately after NDST action. However, it is still unknown whether O-sulfation inhibits NDST action in a similar manner. In this article we radically change conventional assumptions regarding HS biosynthesis by providing in vitro evidence that N-sulfation is not necessarily just a gateway modification during HS biosynthesis.

Link to journal: http://www.febsletters.org/