Li J, Andreyev O, Chen M, Marco M, Iwase H, Long C, Ayares D, Shen Z, Cooper DK, Ezzelarab MB. (2013) Human T cells upregulate CD69 after coculture with xenogeneic genetically-modified pig mesenchymal stromal cells. Cell Immunol. 285(1-2):23-30. PMID: 24044963
Mesenchymal stromal cells (MSC) obtained from α1,3-galactosyltransferase gene knock-out pigs transgenic for the human complement-regulatory protein CD46 (GTKO/CD46 pMSC) suppress in vitro human anti-pig cellular responses as efficiently as allogeneic human MSC. We investigated the immunoregulatory effects of GTKO/CD46 pMSC on human CD4+ and CD8+ T cell proliferation in response to pig aortic endothelial cells (pAEC). pMSC efficiently suppressed T cell proliferation, which was associated with downregulation of granzyme B expression. No induction of CD4+CD25+Foxp3hi regulatory T cells or T cell apoptosis was documented. In correlation with T cell proliferation, CD25 expression was upregulated on T cells in response to pAEC but not to pMSC. In contrast, CD69 expression was upregulated on T cells in response to both pMSC and pAEC, which was associated with a significant increase in the phosphorylation of STAT5. GTKO/CD46 pMSC possibly regulate human T cell responses through modulation of CD69 expression and STAT5 signaling.
Copyright © 2013 Elsevier Inc. All rights reserved.
CD69, GTKO/CD46, GTKO/hCD46, Gal, LAG-3, Mesenchymal stem cells, PBMC, Pig, STAT5, T cells, Xenotransplantation, galactose-α1,3-galactose, lymphocyte activation gene-3, pAEC, pMSC, pSTAT5, peripheral blood mononuclear cells, phosphorylated signal transducer and activator of transcription 5, pig aortic endothelial cells, pig mesenchymal stromal cells, α1,3-galactosyltransferase gene-knockout/human CD46
Link to journal: http://www.journals.elsevier.com/cellular-immunology/
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