Lauer ME, Loftis J, de la Motte C, Hascall VC. Analysis of the heavy-chain modification and TSG-6 activity in pathological hyaluronan matrices. Methods Mol Biol. 2015;1229:543-8. doi: 10.1007/978-1-4939-1714-3_42. PMID: 25325979 PMCID: PMC4397960
During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred to hyaluronan (HA) via the enzyme tumor-necrosis-factor-stimulated-gene 6 (TSG-6) to form a HC-HA complex. In this manuscript, we describe a gel-based assay to detect HC-HA and TSG-6 activity in tissues.