Thomas SN, Zhang H. Expert Rev Proteomics. 2016 Sep 1:1-3. [Epub ahead of print] No abstract available. PMID: 27565203
Fahie K, Zachara NE. J Mol Biol. 2016 Aug 14;428(16):3305-24. doi: 10.1016/j.jmb.2016.06.011. Review. PMID: 2734566 Abstract Glycoconjugates, glycans, carbohydrates, and sugars: these terms encompass a class of biomolecules that are diverse in both form and function ranging from free oligosaccharides, glycoproteins, and … Continued
Hardivillé S, Hart GW. Curr Opin Chem Biol. 2016 Aug;33:88-94. doi: 10.1016/j.cbpa.2016.06.005. Review. PMID: 27322399 Abstract O-GlcNAcylation is a dynamic post-translational modification that is responsive to nutrient availably via the hexosamine biosynthetic pathway and its endproduct UDP-GlcNAc. O-GlcNAcylation serves as a nutrient … Continued
Taparra K, Tran PT, Zachara NE. Front Oncol. 2016 Apr 18;6:85. doi: 10.3389/fonc.2016.00085. Review. PMID: 27148477 Abstract The epithelial-mesenchymal transition (EMT) is a highly conserved program necessary for orchestrating distant cell migration during embryonic development. Multiple studies in cancer have demonstrated a … Continued
Miller WP, Mihailescu ML, Yang C, Barber AJ, Kimball SR, Jefferson LS, Dennis MD. Invest Ophthalmol Vis Sci. 2016 Mar;57(3):1327-37. doi: 10.1167/iovs.15-18719. PMID: 26998719 Abstract PURPOSE: The translational repressor 4E-BP1 interacts with the mRNA cap-binding protein eIF4E and thereby promotes cap-independent translation … Continued
Lagerlöf O, Slocomb JE, Hong I, Aponte Y, Blackshaw S, Hart GW, Huganir RL. Science. 2016 Mar 18;351(6279):1293-6. doi: 10.1126/science.aad5494. PMID: 26989246 Abstract Maintaining energy homeostasis is crucial for the survival and health of organisms. The brain regulates feeding by responding to … Continued
Ma J, Hart GW. Methods Mol Biol. 2016;1410:91-103. doi: 10.1007/978-1-4939-3524-6_6. PMID: 26867740 Abstract The dynamic co- and post-translational modification (PTM) of proteins, O-linked β-D-N-acetylglucosamine modification (O-GlcNAcylation) of serine/threonine residues is critical in many cellular processes, contributing to multiple physiological and pathological events. … Continued
Moore JA, Miller WP, Dennis MD. Cell Signal. 2016 May;28(5):384-90. doi: 10.1016/j.cellsig.2016.01.017. PMID: 26852666 Abstract Resistance to insulin action is a key cause of diabetic complications, yet much remains unknown about the molecular mechanisms that contribute to the defect. Glucose-induced insulin resistance … Continued
Aiyetan P, Thomas SN, Zhang Z, Zhang H. BMC Bioinformatics. 2015 Dec 12;16:411. doi: 10.1186/s12859-015-0838-z. PMID: 26652794 Abstract BACKGROUND: Selected and multiple reaction monitoring involves monitoring a multiplexed assay of proteotypic peptides and associated transitions in mass spectrometry runs. To describe peptide … Continued
Yang S, Wang M, Chen L, Yin B, Song G, Turko IV, Phinney KW, Betenbaugh MJ, Zhang H, Li S. Sci Rep. 2015 Nov 30;5:17585. doi: 10.1038/srep17585. PMID: 26616285 Abstract Glycan is an important class of macromolecules that play numerous biological functions. … Continued
Sun S, Zhang H. Anal Chem. 2015 Dec 15;87(24):11948-51. doi: 10.1021/acs.analchem.5b03886. PMID: 26593774 Abstract It is well-known that N-linked glycans usually attach to asparagine residues in the N-X-S/T motifs of proteins. However, accumulating evidence indicates that N-glycosylation could also possibly occur at … Continued
Uosaki H, Cahan P, Lee DI, Wang S, Miyamoto M, Fernandez L, Kass DA, Kwon C. Cell Rep. 2015 Nov 24;13(8):1705-16. doi: 10.1016/j.celrep.2015.10.032. PMID: 26586429 Abstract Decades of progress in developmental cardiology has advanced our understanding of the early aspects of heart … Continued
CD36 Is a Matrix Metalloproteinase-9 Substrate That Stimulates Neutrophil Apoptosis and Removal During Cardiac Remodeling
DeLeon-Pennell KY, Tian Y, Zhang B, Cates CA, Iyer RP, Cannon P, Shah P, Aiyetan P, Halade GV, Ma Y, Flynn E, Zhang Z, Jin YF, Zhang H, Lindsey ML. Circ Cardiovasc Genet. 2016 Feb;9(1):14-25. doi: 10.1161/CIRCGENETICS.115.001249. PMID: 26578544 Abstract BACKGROUND: After … Continued
Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides
Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Höti N, Zhang Z, Chan DW, Zhang H. Nat Biotechnol. 2016 Jan;34(1):84-8. doi: 10.1038/nbt.3403. PMID: 26571101 Abstract Comprehensive characterization of protein glycosylation is critical for understanding … Continued
Multiplexed Targeted Mass Spectrometry-Based Assays for the Quantification of N-Linked Glycosite-Containing Peptides in Serum
Thomas SN, Harlan R, Chen J, Aiyetan P, Liu Y, Sokoll LJ, Aebersold R, Chan DW, Zhang H. Anal Chem. 2015 Nov 3;87(21):10830-8. doi: 10.1021/acs.analchem.5b02063. PMID: 26451657 Abstract Protein glycosylation is one of the most common protein modifications, and the quantitative analysis … Continued
O-GlcNAcomic Profiling Identifies Widespread O-Linked β-N-Acetylglucosamine Modification (O-GlcNAcylation) in Oxidative Phosphorylation System Regulating Cardiac Mitochondrial Function
Ma J, Liu T, Wei AC, Banerjee P, O’Rourke B, Hart GW. J Biol Chem. 2015 Dec 4;290(49):29141-53. doi: 10.1074/jbc.M115.691741. PMID: 26446791 Abstract Dynamic cycling of O-linked β-N-acetylglucosamine (O-GlcNAc) on nucleocytoplasmic proteins serves as a nutrient sensor to regulate numerous biological processes. … Continued
Kumar A, Baycin-Hizal D, Wolozny D, Pedersen LE, Lewis NE, Heffner K, Chaerkady R, Cole RN, Shiloach J, Zhang H, Bowen MA, Betenbaugh MJ. J Proteome Res. 2015 Nov 6;14(11):4687-703. doi: 10.1021/acs.jproteome.5b00588. PMID: 26418914 Abstract Chinese hamster ovary (CHO) cells are the … Continued
Yang S, Rubin A, Eshghi ST, Zhang H. Proteomics. 2016 Jan;16(2):241-56. doi: 10.1002/pmic.201500266. Review. PMID: 26390280 Abstract Over the past decade, considerable progress has been made with respect to the analytical methods for analysis of glycans from biological sources. Regardless of the … Continued
Integrated glycoprotein immobilization method for glycopeptide and glycan analysis of cardiac hypertrophy
Yang S, Mishra S, Chen L, Zhou JY, Chan DW, Chatterjee S, Zhang H. Anal Chem. 2015 Oct 6;87(19):9671-8. doi: 10.1021/acs.analchem.5b01663. PMID: 26378618 Abstract Post-translational modifications of proteins can have a major role in disease initiation and progression. Incredible efforts have recently … Continued
Identification and biological consequences of the O-GlcNAc modification of the human innate immune receptor, Nod2
Hou CW, Mohanan V, Zachara NE, Grimes CL. Glycobiology. 2016 Jan;26(1):13-8. doi: 10.1093/glycob/cwv076. PMID: 26369908 Abstract Nucleotide-binding oligomerization domain 2 (Nod2) is an intracellular receptor that can sense the bacterial peptidoglycan component, muramyl dipeptide. Upon activation, Nod2 induces the production of various … Continued
Dual Labeling Biotin Switch Assay to Reduce Bias Derived From Different Cysteine Subpopulations: A Method to Maximize S-Nitrosylation Detection
Chung HS, Murray CI, Venkatraman V, Crowgey EL, Rainer PP, Cole RN, Bomgarden RD, Rogers JC, Balkan W, Hare JM, Kass DA, Van Eyk JE. Circ Res. 2015 Oct 23;117(10):846-57. doi: 10.1161/CIRCRESAHA.115.307336. PMID: 26338901 Abstract RATIONALE: S-nitrosylation (SNO), an oxidative post-translational modification … Continued
Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation
Shah P, Wang X, Yang W, Toghi Eshghi S, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A, Zhang H. Mol Cell Proteomics. 2015 Oct;14(10):2753-63. doi: 10.1074/mcp.M115.047928. PMID: 26256267 Abstract Prostate cancer is the most common cancer among men … Continued
Prevention of cardiac hypertrophy by the use of a glycosphingolipid synthesis inhibitor in ApoE-/- mice
Mishra S, Bedja D, Amuzie C, Avolio A, Chatterjee S. Biochem Biophys Res Commun. 2015 Sep 11;465(1):159-64. doi: 10.1016/j.bbrc.2015.07.159. PMID: 26253472 Abstract ApoE-/- mice fed a high fat and high cholesterol (HFHC) diet (20% fat and 1.25% cholesterol) from 12 weeks of … Continued
Improved intervention of atherosclerosis and cardiac hypertrophy through biodegradable polymer-encapsulated delivery of glycosphingolipid inhibitor
Mishra S, Bedja D, Amuzie C, Foss CA, Pomper MG, Bhattacharya R, Yarema KJ, Chatterjee S. Biomaterials. 2015 Sep;64:125-35. doi: 10.1016/j.biomaterials.2015.06.001. PMID: 26111596 Abstract D-Threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP), a glycosphingolipid synthesis inhibitor, holds promise for the treatment of atherosclerosis and cardiac hypertrophy but rapid … Continued
Removal of Abnormal Myofilament O-GlcNAcylation Restores Ca2+ Sensitivity in Diabetic Cardiac Muscle
Ramirez-Correa GA, Ma J, Slawson C, Zeidan Q, Lugo-Fagundo NS, Xu M, Shen X, Gao WD, Caceres V, Chakir K, DeVine L, Cole RN, Marchionni L, Paolocci N, Hart GW, Murphy AM. Diabetes. 2015 Oct;64(10):3573-87. doi: 10.2337/db14-1107. PMID: 26109417 Abstract Contractile dysfunction … Continued
Sun S, Zhang H. Anal Chem. 2015 Jul 7;87(13):6479-82. doi: 10.1021/acs.analchem.5b01679. PMID: 26066578 Abstract Protein glycosylation is one of the most important protein modifications. Glycosylation site occupancy alteration has been implicated in human diseases and cancers. However, current glycoproteomic methods focus on … Continued
Structures of Capsular Polysaccharide Serotypes 35F and 35C of Streptococcus pneumoniae Determined by Nuclear Magnetic Resonance and Their Relation to Other Cross-Reactive Serotypes
Bush CA, Cisar JO, Yang J. J Bacteriol. 2015 Sep;197(17):2762-9. doi: 10.1128/JB.00207-15. PMID: 26055112 Abstract The structures of Streptococcus pneumoniae capsular polysaccharides (CPSs) are essential for defining the antigenic as well as genetic relationships between CPS serotypes. The four serotypes that comprise … Continued
Correction: Overexpression of periostin in stroma positively associated with aggressive prostate cancer
Tian Y, Choi CH, Li QK, Rahmatpanah FB, Chen X, Kim SR, Veltri R, Chia D, Zhang Z, Mercola D, Zhang H. PLoS One. 2015 Jun 4;10(6):e0130333. doi: 10.1371/journal.pone.0130333. No abstract available. PMID: 26043260
Post-translational O-GlcNAcylation is essential for nuclear pore integrity and maintenance of the pore selectivity filter
Zhu Y, Liu TW, Madden Z, Yuzwa SA, Murray K, Cecioni S, Zachara N, Vocadlo DJ. J Mol Cell Biol. 2016 Feb;8(1):2-16. doi: 10.1093/jmcb/mjv033. PMID: 26031751 Abstract O-glycosylation of the nuclear pore complex (NPC) by O-linked N-acetylglucosamine (O-GlcNAc) is conserved within metazoans. … Continued
Application of quantitative proteomics to the integrated analysis of the ubiquitylated and global proteomes of xenograft tumor tissues
Thomas SN, Zhang H, Cotter RJ. Clin Proteomics. 2015 May 20;12(1):14. doi: 10.1186/s12014-015-9086-5. PMID: 26019700 Abstract BACKGROUND: Post-translational modification by ubiquitin is a fundamental regulatory mechanism that is implicated in many cellular processes including the cell cycle, apoptosis, cell adhesion, angiogenesis, and … Continued
Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells
Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H. Clin Proteomics. 2015 Apr 11;12(1):11. doi: 10.1186/s12014-015-9083-8. PMID: 25987888 Abstract In this study, we investigated the use of metabolic oligosaccharide engineering and … Continued
GPQuest: A Spectral Library Matching Algorithm for Site-Specific Assignment of Tandem Mass Spectra to Intact N-glycopeptides
Toghi Eshghi S, Shah P, Yang W, Li X, Zhang H. Anal Chem. 2015;87(10):5181-8. doi: 10.1021/acs.analchem.5b00024. PMID: 25945896 Abstract Glycoprotein changes occur in not only protein abundance but also the occupancy of each glycosylation site by different glycoforms during biological or pathological … Continued
Nakamura T, Ranek MJ, Lee DI, Shalkey Hahn V, Kim C, Eaton P, Kass DA. J Clin Invest. 2015 Jun;125(6):2468-72. doi: 10.1172/JCI80275. PMID: 25938783 Abstract The cGMP-dependent protein kinase-1α (PKG1α) transduces NO and natriuretic peptide signaling; therefore, PKG1α activation can benefit the … Continued
Banerjee PS, Ma J, Hart GW. Proc Natl Acad Sci U S A. 2015 May 12;112(19):6050-5. doi: 10.1073/pnas.1424017112. PMID: 25918408 Abstract Elevated mitochondrial O-GlcNAcylation caused by hyperglycemia, as occurs in diabetes, significantly contributes to mitochondrial dysfunction and to diabetic cardiomyopathy. However, little … Continued
Foss CA, Bedja D, Mease RC, Wang H, Kass DA, Chatterjee S, Pomper MG. Biochem Biophys Res Commun. 2015 May 22;461(1):70-5. doi: 10.1016/j.bbrc.2015.03.171. PMID: 25858322 Abstract BACKGROUND: Atherosclerosis is a common and serious vascular disease predisposing individuals to myocardial infarction and stroke. … Continued
Li Y, Shah P, De Marzo AM, Van Eyk JE, Li Q, Chan DW, Zhang H. Anal Chem. 2015;87(9):4683-7. doi: 10.1021/ac504304v. PMID: 25837443 Abstract Glycosylation is one of the most common protein modifications. Each glycoprotein can be glycosylated at multiple glycosites, and … Continued
Lee DI, Zhu G, Sasaki T, Cho GS, Hamdani N, Holewinski R, Jo SH, Danner T, Zhang M, Rainer PP, Bedja D, Kirk JA, Ranek MJ, Dostmann WR, Kwon C, Margulies KB, Van Eyk JE, Paulus WJ, Takimoto E, Kass … Continued
Tian Y, Choi CH, Li QK, Rahmatpanah FB, Chen X, Kim SR, Veltri R, Chia D, Zhang Z, Mercola D, Zhang H.PLoS One. 2015 Mar 17;10(3):e0121502. doi: 10.1371/journal.pone.0121502. Erratum in: PLoS One. 2015;10(6):e0130333. PMID: 25781169 Abstract BACKGROUND: Periostin is an important … Continued
O-linked β-N-acetylglucosamine transferase directs cell proliferation in idiopathic pulmonary arterial hypertension
Barnes JW, Tian L, Heresi GA, Farver CF, Asosingh K, Comhair SA, Aulak KS, Dweik RA. Circulation. 2015 Apr 7;131(14):1260-8. doi: 10.1161/CIRCULATIONAHA.114.013878. PMID: 25663381 Abstract BACKGROUND: Idiopathic pulmonary arterial hypertension (IPAH) is a cardiopulmonary disease characterized by cellular proliferation and vascular remodeling. … Continued
Emerging proteomic technologies for elucidating context-dependent cellular signaling events: A big challenge of tiny proportions
Parker SJ, Raedschelders K, Van Eyk JE. Proteomics. 2015 May;15(9):1486-502. doi: 10.1002/pmic.201400448. Review. PMID: 25545106 Abstract Aberrant cell signaling events either drive or compensate for nearly all pathologies. A thorough description and quantification of maladaptive signaling flux in disease is a critical … Continued
Three Decades of Research on O-GlcNAcylation – A Major Nutrient Sensor That Regulates Signaling, Transcription and Cellular Metabolism
Hart GW. Front Endocrinol (Lausanne). 2014 Oct 27;5:183. doi: 10.3389/fendo.2014.00183. Review. PMID: 25386167 Abstract Even though the dynamic modification of polypeptides by the monosaccharide, O-linked N-acetylglucosamine (O-GlcNAcylation) was discovered over 30 years ago, its physiological significance as a major nutrient sensor that regulates … Continued
Kageyama Y, Hoshijima M, Seo K, Bedja D, Sysa-Shah P, Andrabi SA, Chen W, Höke A, Dawson VL, Dawson TM, Gabrielson K, Kass DA, Iijima M, Sesaki H. EMBO J. 2014 Dec 1;33(23):2798-813. doi: 10.15252/embj.201488658. PMID: 25349190 Abstract Mitochondrial dynamics and mitophagy … Continued
Minireview series on the thirtieth anniversary of research on O-GlcNAcylation of nuclear and cytoplasmic proteins: Nutrient regulation of cellular metabolism and physiology by O-GlcNAcylation
Hart GW. J Biol Chem. 2014 Dec 12;289(50):34422-3. doi: 10.1074/jbc.R114.609776. PMID: 25336646 Abstract The dynamic cycling of N-acetylglucosamine (termed O-GlcNAcylation) on serine or threonine residues of nuclear or cytoplasmic proteins serves as a nutrient sensor, both independently and also via its interplay … Continued
Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou JY, Ao M, Moore RD, Jackson JB, Zhang H. Theranostics. 2014 Sep 18;4(12):1153-63. doi: 10.7150/thno.9510. PMID: 25285165 Abstract HIV elite suppressors (ES) or controllers are individuals achieving control of viremia by … Continued
Shah P, Zhang B, Choi C, Yang S, Zhou J, Harlan R, Tian Y, Zhang Z, Chan DW, Zhang H. Anal Biochem. 2015 Jan 15;469:27-33. doi: 10.1016/j.ab.2014.09.017. PMID: 25283129 Abstract Proteomics analysis is important for characterizing tissues to gain biological and pathological … Continued
Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway
Zhong J, Martinez M, Sengupta S, Lee A, Wu X, Chaerkady R, Chatterjee A, O’Meally RN, Cole RN, Pandey A, Zachara NE. Proteomics. 2015 Jan;15(2-3):591-607. doi: 10.1002/pmic.201400339. PMID: 25263469 Abstract The modification of intracellular proteins by monosaccharides of O-linked β-N-acetylglucosamine (O-GlcNAc) is … Continued
Yang S, Chen L, Sun S, Shah P, Yang W, Zhang B, Zhang Z, Chan DW, Kass DA, van Eyk JE, Zhang H. Proteomics. 2015 Jan;15(2-3):567-79. doi: 10.1002/pmic.201400151. PMID: 25141849 Abstract Conduction abnormalities can lead to dyssynchronous contraction, which significantly worsens morbidity … Continued
Hardivillé S, Hart GW. Cell Metab. 2014 Aug 5;20(2):208-13. doi: 10.1016/j.cmet.2014.07.014. Review. PMID: 25100062 Abstract The nutrient sensor, O-linked N-acetylglucosamine (O-GlcNAc), cycles on and off nuclear and cytosolic proteins to regulate many cellular processes, including transcription and signaling. Dysregulated O-GlcNAcylation and its interplay with … Continued
Imaging of N-linked glycans from formalin-fixed paraffin-embedded tissue sections using MALDI mass spectrometry
Toghi Eshghi S, Yang S, Wang X, Shah P, Li X, Zhang H. ACS Chem Biol. 2014 Sep 19;9(9):2149-56. doi: 10.1021/cb500405h. PMID: 25029481 Abstract Aberrant glycosylation is associated with most of the diseases. Direct imaging and profiling of N-glycans on tissue sections … Continued
Chemical structures of Streptococcus pneumoniae capsular polysaccharide type 39 (CPS39), CPS47F, and CPS34 characterized by nuclear magnetic resonance spectroscopy and their relation to CPS10A
Bush CA, Yang J, Yu B, Cisar JO. J Bacteriol. 2014 Sep;196(18):3271-8. doi: 10.1128/JB.01731-14. PMID: 25002537 Abstract Structural characterization of Streptococcus pneumoniae capsular polysaccharides (CPS) is a prerequisite for unraveling both antigenic and genetic relationships that exist between different serotypes. In the … Continued
Physiologic and pathophysiologic consequences of altered sialylation and glycosylation on ion channel function
Baycin-Hizal D, Gottschalk A, Jacobson E, Mai S, Wolozny D, Zhang H, Krag SS, Betenbaugh MJ. Biochem Biophys Res Commun. 2014 Oct 17;453(2):243-53. doi: 10.1016/j.bbrc.2014.06.067. Review. PMID: 24971539 Abstract Voltage-gated ion channels are transmembrane proteins that regulate electrical excitability in cells and … Continued
Glycoform analysis of recombinant and human immunodeficiency virus envelope protein gp120 via higher energy collisional dissociation and spectral-aligning strategy
Yang W, Shah P, Toghi Eshghi S, Yang S, Sun S, Ao M, Rubin A, Jackson JB, Zhang H. Anal Chem. 2014 Jul 15;86(14):6959-67. doi: 10.1021/ac500876p. PMID: 24941220 Abstract Envelope protein gp120 of human immunodeficiency virus (HIV) is armored with a dense … Continued
Tian Y, Koganti T, Yao Z, Cannon P, Shah P, Pietrovito L, Modesti A, Aiyetan P, DeLeon-Pennell K, Ma Y, Halade GV, Hicks C, Zhang H, Lindsey ML. Proteomics Clin Appl. 2014 Aug;8(7-8):595-602. doi: 10.1002/prca.201400009. PMID: 24920555 Abstract PURPOSE: Extracellular proteins are … Continued
Wang X, Chen J, Li QK, Peskoe SB, Zhang B, Choi C, Platz EA, Zhang H. Glycobiology. 2014 Oct;24(10):935-44. doi: 10.1093/glycob/cwu051. PMID: 24906821 Abstract Aberrant protein glycosylation is known to be associated with the development of cancers. The aberrant glycans are produced … Continued
Shenje LT, Andersen P, Uosaki H, Fernandez L, Rainer PP, Cho GS, Lee DI, Zhong W, Harvey RP, Kass DA, Kwon C. Elife. 2014 Apr 24;3:e02164. doi: 10.7554/eLife.02164. PMID: 24843018 Abstract Cardiac progenitor cells (CPCs) must control their number and fate to … Continued
Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness
Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R. Mol Cell Proteomics. 2014 Jul;13(7):1753-68. doi: 10.1074/mcp.M114.038273. PMID: 24741114 Abstract The identification of biomarkers indicating the level of aggressiveness of prostate cancer … Continued
Inhibition of glycosphingolipid synthesis ameliorates atherosclerosis and arterial stiffness in apolipoprotein E-/- mice and rabbits fed a high-fat and -cholesterol diet
Chatterjee S, Bedja D, Mishra S, Amuzie C, Avolio A, Kass DA, Berkowitz D, Renehan M. Circulation. 2014 Jun 10;129(23):2403-13. doi: 10.1161/CIRCULATIONAHA.113.007559. PMID: 24710030 Abstract BACKGROUND: Glycosphingolipids, integral components of the cell membrane, have been shown to serve as messengers, transducing growth … Continued
Semba RD, Huang H, Lutty GA, Van Eyk JE, Hart GW. Proteomics Clin Appl. 2014 Apr;8(3-4):218-31. doi: 10.1002/prca.201300076. Review. PMID: 24550151 Abstract Diabetic retinopathy is a leading cause of blindness worldwide. Despite laser and surgical treatments, antiangiogenic and other therapies, and strict … Continued
Epithelium percentage estimation facilitates epithelial quantitative protein measurement in tissue specimens
Chen J, Toghi Eshghi S, Bova GS, Li QK, Li X, Zhang H. Clin Proteomics. 2013 Dec 1;10(1):18. doi: 10.1186/1559-0275-10-18. PMID: 24289299 Abstract BACKGROUND: The rapid advancement of high-throughput tools for quantitative measurement of proteins has demonstrated the potential for the identification … Continued
Hart GW, Ball LE. Mol Cell Proteomics. 2013 Dec;12(12):3443. doi: 10.1074/mcp.E113.036491. No abstract available. PMID: 24277940
Sun S, Zhou JY, Yang W, Zhang H. Anal Biochem. 2014 Feb 1;446:76-81. doi: 10.1016/j.ab.2013.10.024. PMID: 24161613 Abstract Urea solution is one of the most commonly employed protein denaturants for protease digestion in proteomic studies. However, it has long been recognized that … Continued
Hardivillé S, Hart GW.Curr Opin Chem Biol. 2016 Aug;33:88-94. doi: 10.1016/j.cbpa.2016.06.005. Review. PMID: 27322399 Abstract O-GlcNAcylation is a dynamic post-translational modification that is responsive to nutrient availably via the hexosamine biosynthetic pathway and its endproduct UDP-GlcNAc. O-GlcNAcylation serves as a nutrient … Continued
Fahie K, Zachara NE. J Mol Biol. 2016 Aug 14;428(16):3305-24. doi: 10.1016/j.jmb.2016.06.011. Review. PMID: 27345664 Abstract Glycoconjugates, glycans, carbohydrates, and sugars: these terms encompass a class of biomolecules that are diverse in both form and function ranging from free oligosaccharides, glycoproteins, and … Continued
Thomas SN, Zhang H. Expert Rev Proteomics. 2016 Sep 1:1-3. PMID: 27565203
Harlan R, Zhang H. (2014) Targeted proteomics: a bridge between discovery and validation. Expert Rev Proteomics. 11(6):657-61. PMID: 25348939. Abstract New technologies in mass spectrometry are beginning to mature and show unique advantages for the identification and quantitation of proteins. … Continued
Soluble Guanylate Cyclase Is Required for Systemic Vasodilation But Not Positive Inotropy Induced by Nitroxyl in the Mouse.
Zhu G, Groneberg D, Sikka G, Hori D, Ranek MJ, Nakamura T, Takimoto E, Paolocci N, Berkowitz DE, Friebe A, Kass DA.(2014) Soluble Guanylate Cyclase Is Required for Systemic Vasodilation But Not Positive Inotropy Induced by Nitroxyl in the Mouse. … Continued
XGlycScan: An Open-source Software For N-linked Glycosite Assignment, Quantification and Quality Assessment of Data from Mass Spectrometry-based Glycoproteomic Analysis.
Aiyetan P, Zhang B, Zhang Z, Zhang H. (2014) XGlycScan: An Open-source Software For N-linked Glycosite Assignment, Quantification and Quality Assessment of Data from Mass Spectrometry-based Glycoproteomic Analysis. MOJ Proteom Bioinform. 2014;1(1). pii: 00004. PMID: 25346946. PMCID: PMC4207213. Abstract Mass … Continued
Glycomic analysis of glycans released from glycoproteins using chemical immobilization and mass spectrometry
Yang S, Zhang H.(2014) Glycomic analysis of glycans released from glycoproteins using chemical immobilization and mass spectrometry. Curr Protoc Chem Biol. 6(3):191-208. PMID: 25205566. PMCID: PMC4171959. Abstract Protein glycosylation is one of most common protein modifications and is involved in … Continued
Stéphan Hardivillé and Gerald W. Hart (2014) Nutrient Regulation of Transcription, Signaling, and Cell Physiology by O-GlcNAcylation. Cell Metab. 20:208-213. PMID: 25100062. Abstract The nutrient sensor, O-linked N-acetylglucosamine (O-GlcNAc), cycles on and off nuclear and cytosolic proteins to regulate many … Continued
Chatterjee S, Mishra S, Suzuki K. Lactosylceramdie :new vis-tas of research. Adv Expt Med 2014 (In Press) Link to journal: http://www.springer.com/series/5584
Characterization of the specificity of O-GlcNAc reactive antibodies under conditions of starvation and stress.
Reeves RA, Lee A, Henry R, Zachara NE. (2014) Characterization of the specificity of O-GlcNAc reactive antibodies under conditions of starvation and stress. Anal Biochem. 457:8-18. PMID: 24747005. Abstract The dynamic modification of nuclear, cytoplasmic, and mitochondrial proteins by O-linked … Continued
The utility of a novel triple marker (combination of TTF1, napsin A, and p40) in the subclassification of non-small cell lung cancer.
Ao MH, Zhang H, Sakowski L, Sharma R, Illei PB, Gabrielson E, Askin F, Li QK. (2014) The utility of a novel triple marker (combination of TTF1, napsin A, and p40) in the subclassification of non-small cell lung cancer. Hum … Continued
Cross-talk between two essential nutrient-sensitive enzymes: O-GlcNAc transferase (OGT) and AMP-activated protein kinase (AMPK).
Bullen JW, Balsbaugh JL, Chanda D, Shabanowitz J, Hunt DF, Neumann D, Hart GW. (2014) Cross-talk between two essential nutrient-sensitive enzymes: O-GlcNAc transferase (OGT) and AMP-activated protein kinase (AMPK). J Biol Chem. 289(15):10592-606. PMID: 24563466; PMCID: PMC4036179 Abstract Nutrient-sensitive pathways regulate both O-GlcNAc transferase (OGT) and AMP-activated protein kinase (AMPK), cooperatively connecting … Continued
Identification of Cardiac Myofilament Protein Isoforms Using Multiple Mass Spectrometry Based Approaches.
Kooij V, Venkatraman V, Kirk JA, Ubaida-Mohien C, Graham DR, Faber MJ, Van Eyk JE. (2014) Identification of Cardiac Myofilament Protein Isoforms Using Multiple Mass Spectrometry Based Approaches. PMID: 24974818. Abstract PURPOSE: The identification of protein isoforms in complex biological … Continued
Inhibition of glycosphingolipid synthesis ameliorates atherosclerosis and arterial stiffness in apolipoprotein e-/- mice and rabbits fed a high-fat and -cholesterol diet.
Chatterjee S, Bedja D, Mishra S, Amuzie C, Avolio A, Kass DA, Berkowitz D, Renehan M. (2014) Inhibition of glycosphingolipid synthesis ameliorates atherosclerosis and arterial stiffness in apolipoprotein e-/- mice and rabbits fed a high-fat and -cholesterol diet. Circulation. 129(23):2403-13. … Continued
Lactosylceramide promotes hypertrophy through ROS generation and activation of ERK1/2 in cardiomyocytes.
Mishra S, Chatterjee S. (2014) Lactosylceramide promotes hypertrophy through ROS generation and activation of ERK1/2 in cardiomyocytes. Glycobiology. 24(6):518-31. PMID: 24658420. PMCID: PMC4001711 Abstract Hypertrophy is central to several heart diseases; however, not much is known about the role of … Continued
Sasaki H, Nagayama T, Blanton RM, Seo K, Zhang M, Zhu G, Lee DI, Bedja D, Hsu S, Tsukamoto O, Takashima S, Kitakaze M, Mendelsohn ME, Karas RH, Kass DA, Takimoto E. (2014) PDE5 inhibitor efficacy is estrogen dependent in … Continued
Aiyetan P, Zhang B, Chen L, Zhang Z, Zhang H.(2014) M2Lite: An Open-source, Light-weight, Pluggable and Fast Proteome Discoverer MSF to mzIdentML Tool. J Bioinform. 1(2):40-49. PMID: 25346941. PMCID: PMC4206089. Abstract Proteome Discoverer is one of many tools used for … Continued
Groves JA1, Lee A, Yildirir G, Zachara NE. (2013) Dynamic O-GlcNAcylation and its roles in the cellular stress response and homeostasis. Cell Stress Chaperones. 18(5):535-58. PMID: 23620203; PMCID: PMC3745259 Abstract O-linked N-acetyl-β-D-glucosamine (O-GlcNAc) is a ubiquitous and dynamic post-translational modification known to modify over 3,000 nuclear, cytoplasmic, and mitochondrial … Continued
Nutrient regulation of immunity: O-GlcNAcylation regulates stimulus-specific NF-κB-dependent transcription.
Hart GW. (2013) Nutrient regulation of immunity: O-GlcNAcylation regulates stimulus-specific NF-κB-dependent transcription. Sci Signal. 6(290):pe26. PMID: 23982203 Abstract Nutrients regulate gene transcription by the dynamic cycling of O-linked N-acetylglucosamine (O-GlcNAc) on proteins that constitute the transcriptional machinery. A study shows that O-GlcNAcylation … Continued
Baycin Hizal D, Wolozny D, Colao J, Jacobson E, Tian Y, Krag SS, Betenbaugh MJ, Zhang H. (2014) Glycoproteomic and glycomic databases. Clin Proteomics. 11(1):15. PMID: 24725457. PMCID: PMC3996109. Abstract Protein glycosylation serves critical roles in the cellular and biological … Continued
Hascall VC, Wang A, Tammi M, Oikari S, Tammi R, Passi A, Vigetti D, Hanson RW, Hart GW. (2014) The dynamic metabolism of hyaluronan regulates the cytosolic concentration of UDP-GlcNAc. Matrix Biol. 35:14-7. PMID: 24486448. PMCID: PMC4039572. Abstract Hyaluronan, a … Continued
Kelly KM, Tocchetti CG, Lyashkov A, Tarwater PM, Bedja D, Graham DR, Beck SE, Metcalf Pate KA, Queen SE, Adams RJ, Paolocci N, Mankowski JL. (2014) CCR5 inhibition prevents cardiac dysfunction in the SIV/macaque model of HIV. J Am Heart … Continued
Ma J, Hart GW. (2014) O-GlcNAc profiling: from proteins to proteomes. Clin Proteomics. 11(1):8. PMID: 24593906. PMCID: PMC4015695. Abstract O-linked β-D-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) onto serine and threonine residues of proteins is an important post-translational modification (PTM), which is involved … Continued
Hyperactive adverse mechanical stress responses in dystrophic heart are coupled to transient receptor potential canonical 6 and blocked by cGMP-protein kinase G modulation.
Seo K, Rainer PP, Lee DI, Hao S, Bedja D, Birnbaumer L, Cingolani OH, Kass DA. (2014) Hyperactive adverse mechanical stress responses in dystrophic heart are coupled to transient receptor potential canonical 6 and blocked by cGMP-protein kinase G modulation. … Continued
Glycoproteomics using fluid-based specimens in the discovery of lung cancer protein biomarkers: promise and challenge.
Li QK, Gabrielson E, Askin F, Chan DW, Zhang H. (2013) Glycoproteomics using fluid-based specimens in the discovery of lung cancer protein biomarkers: promise and challenge. Proteomics Clin Appl. 7(1-2):55-69. PMID: 23112109 Abstract Lung cancer is the leading cancer in the United States and worldwide. In spite of the … Continued
Sun S, Zhou JY, Yang W, Zhang H. (2014) Inhibition of protein carbamylation in urea solution using ammonium-containing buffers. Anal Biochem. 446:76-81. PMID: 24161613. PMCID: PMC4072244. Abstract Urea solution is one of the most commonly employed protein denaturants for protease … Continued
Yang S, Zhang H. (2012) Solid-phase glycan isolation for glycomics analysis. Proteomics Clin Appl. 6(11-12):596-608. PMID: 23090885; PMCID: PMC3674833. Abstract Glycosylation is one of the most significant protein PTMs. The biological activities of proteins are dramatically changed by the glycans associated with them. Thus, structural analysis … Continued
Vibjerg Jensen R, Johnsen J, Buus Kristiansen S, Zachara NE, Bøtker HE. Ischemic preconditioning increases myocardial O-GlcNAc glycosylation. (2013) Scand Cardiovasc J. 47(3):168-74. PMID: 23301939. Abstract OBJECTIVES: Through the hexosamine biosynthetic pathway (HBP) proteins are modified by O-linked-β-N-acetylglucosamine (O-GlcNAc), which acts as a stress sensor. Augmentation of O-GlcNAc … Continued
Chatterjee S, Alsaeedi N, Hou J, Bandaru VV, Wu L, Halushka MK, Pili R, Ndikuyeze G, Haughey NJ. Use of a glycolipid inhibitor to ameliorate renal cancer in a mouse model. (2013) PLoS One. 8(5):e63726. PMID: 23671696; PMCID: PMC3650082. Abstract In a xenograft model wherein, live renal cancer cells were implanted under … Continued
Shah P, Yang S, Sun S, Aiyetan P, Yarema KJ, Zhang H. (2013) Mass spectrometric analysis of sialylated glycans with use of solid-phase labeling of sialic acids. Anal Chem. 2;85(7):3606-13. PMID: 23445396; PMCID: PMC3681956 Abstract The analysis of sialylated glycans is critical for understanding the role of sialic acid in normal … Continued
Sun S, Zhang B, Aiyetan P, Zhou JY, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H. (2014) Analysis of N-glycoproteins using genomic N-glycosite prediction. J Proteome Res. 12:5609-15. PMID: 24164404. PMCID: PMC4072220. Abstract Protein glycosylation has … Continued
Chen J, Shah P, Zhang H. (2013) Solid phase extraction of N-linked glycopeptides using hydrazide tip. Anal Chem. 85(22):10670-4. PMID: 24079330. PMCID: PMC4082910. Abstract Glycoproteome contains valuable information where biomarkers may be discovered for disease diagnosis and monitoring. Nowadays, with … Continued
Banerjee PS, Hart GW, Cho JW. (2013) Chemical approaches to study O-GlcNAcylation. Chem Soc Rev. 42(10):4345-57. PMID: 23247267; PMCID: PMC3641162 Abstract The enzymatic addition of a single β-D-N-acetylglucosamine sugar molecule on serine and/or threonine residues of protein chains is referred to as O-GlcNAcylation. This novel form of post-translational … Continued
Glycomic analysis by glycoprotein immobilization for glycan extraction and liquid chromatography on microfluidic chip.
Yang S, Toghi Eshghi S, Chiu H, DeVoe DL, Zhang H. (2013) Glycomic analysis by glycoprotein immobilization for glycan extraction and liquid chromatography on microfluidic chip. Anal Chem. 85(21):10117-25. PMID: 24111616. PMCID: PMC3867136. Abstract Glycosylation is one of the most … Continued
Ma J, Hart GW. (2013) Protein O-GlcNAcylation in diabetes and diabetic complications. Expert Rev Proteomics. 10(4):365-80. PMID: 23992419 Abstract The post-translational modification of serine and threonine residues of proteins by O-linked β-N-acetylglucosamine (O-GlcNAc) is highly ubiquitous, dynamic and inducible. Protein O-GlcNAcylation serves as a … Continued
Ramirez-Correa GA, Ferrando IM, Hart G, Murphy A. (2013) Detection of O-GlcNAc modifications on cardiac myofilament proteins. Methods Mol Biol. 2013;1005:157-68. doi: 10.1007/978-1-62703-386-2_13. PMID: 23606256. Abstract In this chapter it is described a general method that has been used successfully … Continued
Erickson JR, Pereira L, Wang L, Han G, Ferguson A, Dao K, Copeland RJ, Despa F, Hart GW, Ripplinger CM, Bers DM. (2013) Nature. 502(7471):372-6. PMID: 24077098; PMCID: PMC3801227 Abstract Ca2+/calmodulin-dependent protein kinase II (CaMKII) is an enzyme with important regulatory functions in the heart and brain, and its chronic activation can … Continued
McNeal CJ, Chatterjee S, Hou J, Worthy LS, Larner CD, Macfarlane RD, Alaupovic P, Brocia RW. (2013) Human HDL containing a novel apoC-I isoform induces smooth muscle cell apoptosis. Cardiovasc Res. 1;98(1):83-93 PMID: 23354389; PMCID: PMC3598419 Abstract AIMS: We discovered that some adults with coronary heart disease (CHD) have a high density … Continued
Yang S, Yuan W, Yang W, Zhou J, Harlan R, Edwards J, Li S, Zhang H. (2013) Glycan analysis by isobaric aldehyde reactive tags and mass spectrometry. Anal Chem. 85(17):8188-95. PMID: 23895018. PMCID: PMC3785247. Abstract Glycans play significant roles in … Continued
Tian Y, Zhang H. (2013) Characterization of disease-associated N-linked glycoproteins. Proteomics. 13(3-4):504-11. PMID: 23255236; PMCID: PMC3733363 Abstract N-linked glycoproteins play important roles in biological processes, including cell-to-cell recognition, growth, differentiation, and programmed cell death. Specific N-linked glycoprotein changes are associated with disease progression and identification of these … Continued
Impact of O-GlcNAc on cardioprotection by remote ischaemic preconditioning in non-diabetic and diabetic patients.
Jensen RV, Zachara NE, Nielsen PH, Kimose HH, Kristiansen SB, Bøtker HE. (2013) Impact of O-GlcNAc on cardioprotection by remote ischaemic preconditioning in non-diabetic and diabetic patients. Cardiovasc Res. 97(2):369-78. PMID: 23201773; PMCID: PMC3584969 Abstract AIMS: Post-translational modification of proteins by O-linked β-N-acetylglucosamine (O-GlcNAc) is cardioprotective but its role in … Continued
Yang S, Li Y, Shah P, Zhang H. (2013) Glycomic Analysis Using Glycoprotein Immobilization for Glycan Extraction. Anal Chem. 85(11):5555-61. PMID: 23688297; PMCID: PMC3696186 Abstract Glycosylation is one of the most common protein modifications and is involved in many functions of glycoproteins. Investigating aberrant protein glycosylation associated with … Continued
O-GlcNAcomics-Revealing roles of O-GlcNAcylation in disease mechanisms and development of potential diagnostics.
Copeland RJ, Han G, Hart GW. (2013) O-GlcNAcomics-Revealing roles of O-GlcNAcylation in disease mechanisms and development of potential diagnostics. Proteomics Clin Appl. 2013 May 3. doi: 10.1002/prca.201300001. PMID: 23640805. PMCID: PMC3883918. Abstract O-linked-β-N-acetylglucosamine (O-GlcNAc) is a dynamic PTM of the … Continued
Extracellular and intracellular esterase processing of SCFA-hexosamine analogs: implications for metabolic glycoengineering and drug delivery.
Mathew MP, Tan E, Shah S, Bhattacharya R, Adam Meledeo M, Huang J, Espinoza FA, Yarema KJ. (2012) Extracellular and intracellular esterase processing of SCFA-hexosamine analogs: implications for metabolic glycoengineering and drug delivery. Bioorg Med Chem Lett. 22(22):6929-33. PMID: 23041156. PMCID: PMC3530194 Abstract This report provides a synopsis of the esterase processing … Continued
Targeted analyte detection by standard addition improves detection limits in matrix-assisted laser desorption/ionization mass spectrometry.
Toghi Eshghi S, Li X, Zhang H. (2012) Targeted analyte detection by standard addition improves detection limits in matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem. 84(18):7626-32. PMID: 22877355 Abstract Matrix-assisted laser desorption/ionization (MALDI) has proven an effective tool for fast and accurate determination … Continued
Modification of RelA by O-linked N-acetylglucosamine links glucose metabolism to NF-kB acetylation and transcription.
Allison DF, Wamsley JJ, Kumar M, Li D, Gray LG, Hart GW, Jones DR, Mayo MW. (2012) Modification of RelA by O-linked N-acetylglucosamine links glucose metabolism to NF-κB acetylation and transcription. Proc Natl Acad Sci U S A. 109(42):16888-93. PMID: 23027940. PMCID: PMC3479489 Abstract The molecular mechanisms linking glucose metabolism with active … Continued
Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis.
Almaraz RT, Tian Y, Bhattarcharya R, Tan E, Chen SH, Dallas MR, Chen L, Zhang Z, Zhang H, Konstantopoulos K, Yarema KJ. (2012) Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis. Mol Cell Proteomics. 11(7):M112.017558. PMID: 22457533, PMCID: PMC3394959 Abstract … Continued
Glutathione/thioredoxin systems modulate mitochondrial H2O2 emission: an experimental-computational study.
Aon MA, Stanley BA, Sivakumaran V, Kembro JM, O’Rourke B, Paolocci N, Cortassa S. (2012) Glutathione/thioredoxin systems modulate mitochondrial H2O2 emission: an experimental-computational study. J Gen Physiol. 139(6):479-91. PMID: 22585969; PMCID: PMC3362521 Abstract The net emission of hydrogen peroxide (H(2)O(2)) … Continued
Dias WB, Cheung WD, Hart GW. (2012) O-GlcNAcylation of kinases. Biochem Biophys Res Commun. 422(2):224-8. PMID: 22564745; PMCID: PMC3387735 Abstract Recent evidence indicates that site-specific crosstalk between O-GlcNAcylation and phosphorylation and the O-GlcNAcylation of kinases play an important role in … Continued
Zachara NE. (2012) The roles of O-linked β-N-acetylglucosamine in cardiovascular physiology and disease. Am J Physiol Heart Circ Physiol. 302(10):H1905-18. PMID: 22287582; PMCID: PMC3362101 Abstract More than 1,000 proteins of the nucleus, cytoplasm, and mitochondria are dynamically modified by O-linked … Continued
Tandem mass spectrometry identifies many mouse brain O-GlcNAcylated proteins including EGF domain-specific O-GlcNAc transferase targets.
Alfaro JF, Gong CX, Monroe ME, Aldrich JT, Clauss TR, Purvine SO, Wang Z, Camp DG 2nd, Shabanowitz J, Stanley P, Hart GW, Hunt DF, Yang F, Smith RD. (2012) Tandem mass spectrometry identifies many mouse brain O-GlcNAcylated proteins including … Continued
Zachara NE, Vosseller K, Hart GW. (2011) Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Protein Sci. Chapter 12:Unit12.8. PMID: 22045558; PMCID: PMC3349994 Abstract O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that … Continued