Mass spectrometric analysis of sialylated glycans with use of solid-phase labeling of sialic acids.

Shah P, Yang S, Sun S, Aiyetan P, Yarema KJ, Zhang H. (2013) Mass spectrometric analysis of sialylated glycans with use of solid-phase labeling of sialic acids. Anal Chem. 2;85(7):3606-13. PMID: 23445396; PMCID: PMC3681956

Abstract

The analysis of sialylated glycans is critical for understanding the role of sialic acid in normal biological processes as well as in disease. However, the labile nature of sialic acid typically renders routine analysis of this monosaccharide by mass spectrometric methods difficult. To overcome this difficulty we pursued derivatization methodologies, extending established acetohydrazide approaches to aniline-based methods, and finally to optimized p-toluidine derivatization. This new quantitative glycoform profiling method with use of MALDI-TOF in positive ion mode was validated by first comparing N-glycans isolated from fetuin and serum and was then exploited to analyze the effects of increased metabolic flux through the sialic acid pathway in SW1990 pancreatic cancer cells by using a colabeling strategy with light and heavy toluidine. The latter results established that metabolic flux, in a complementary manner to the more well-known

Link to journal: http://pubs.acs.org/journal/ancham