Zachara NE, Vosseller K, Hart GW. (2011) Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Protein Sci. Chapter 12:Unit12.8. PMID: 22045558; PMCID: PMC3349994
O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer’s disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites.
Link to journal: http://onlinelibrary.wiley.com/book/10.1002/0471140864