Shared Resources

Glycoanalytic Component

This core resource will provide comprehensive sequence profiling for protein and lipid glycans on cells. The goal is to provide detailed structural information (i.e., not diagrams of MS peaks). Link to BWH webpage

Costs: Service and technician fees

Availability: Immediate

Personnel

  • Vernon Reinhold, PhD (Core Director)
  • (603) 862-2527


Adhesion Assay Component

This core resource will perform cell adhesion assays to measure binding of activity of integrins (e.g., VLA-4, VLA-5, LFA-1), of CD44 to hyaluronic acid, and of selectin ligands. This component will also perform shear-based adhesion assays to assess mediators of binding interactions under shear conditions.

Costs: Agreement of collaboration

Availability: Immediate

Personnel


Ex Vivo Glycan Engineering Component

This core resource will utilize newly developed glycosyltransferase reagents and attendant buffers expressly formulated to prevent cell toxicity, to perform stereospecific glycan modification of the surfaces of living cells.

Costs: Agreement of collaboration

Availability: Immediate

Personnel


In Vitro Glycosyltransferase and Glycosidase Assay Component

This component will perform measurement of glycosyltransferase and glycosidase activities in cellular homogenates.

Costs: Agreement of collaboration

Availability: Immediate

Personnel