Projects

Biosynthesis and Function of Lactosaminyl Glycans in Hematopoiesis

Project 1: Function of Lactosaminyl Glycans in Early Hematopoietic and Myelopoietic Cells

aims to utilize glycan profiling together with protein and lipid analytical techniques to characterize the native lactosaminyl glycans displayed on early human hematopoietic cells, and will study how enforced generation of these structures by ex vivo glycan engineering affects myelopoiesis. Link to BWH Webpage

Personnel

Project 2: Regulation of Lactosaminyl Glycan Biosynthesis in Hematopoietic Cells

aims to explore the structural biology and function of lactosaminyl glycans on murine hematopoietic cells, and how these structures are created by intrinsic (intracellular glycosylation) and extrinsic (extracellular glycosylation) biosynthetic routes. Together with Project 1, Project 2 will define the relevant protein and lipid scaffolds presenting lactosaminyl glycans on mouse hematopoietic cells, and will examine how glycosyltransferases and glycosidases regulate lactosaminyl glycan synthesis on human hematopoietic progenitors. Link to BWH Webpage

Personnel

Project 3: Lactosaminyl Glycans in Megakaryocytes and Thrombopoiesis

aims to define the dynamic regulation of lactosaminyl glycan expression in megakaryocytes, with emphasis on understanding the functional consequences of lactosaminyl glycan expression in proplatelet production. Together with Projects 1 and 2, Project 3 will examine the relevant scaffolds displaying lactosaminyl glycans on megakaryocytes and platelets, and will determine how expression of discrete lactosaminyl glycans varies between thrombopoietic and myelopoietic cells and how programmed remodeling of surface glycans can be exploited to regulate thrombopoiesis and myelopoiesis. Link to BWH Webpage

Personnel