Any scientist looking for an improved strategy for the successful characterization of glycoproteins cannot afford to miss this educational webinar!
Glycosylation of proteins is a highly significant post-translational modification that can modulate both protein structure and function. The glycosylation of biotherapeutics is understandably then a structural feature that must be thoroughly characterized and monitored. A commonly employed method for evaluating the N-linked oligosaccharides present on a glycoprotein involves the release of the N-glycan by PNGase F, labeling those oligosaccharides with fluorescently active 2-aminobenzamide (2-AB), subsequent separation via hydrophilic interaction chromatography (HILIC), and detection by fluorescence (FLR).
During the presentation, covered topics will include:
1. Introduction of GlycoWorks™, a convenient N-glycan sample preparation kit from Waters using HILIC solid phase extraction (SPE) for sample clean-up.
2. Principles and practice of Waters ACQUITY UPLC Glycan Column for the HILIC-based chromatography of the labeled N-linked oligosaccharides and its ability to deliver highly resolved and reproducible separations of the neutral and charged glycan species contained in relevant samples.
3. Also to be discussed will be use of HILIC-based separations for the analysis of glycopeptides, as well as a brief overview of structural characterization strategies for N-linked oligosaccharides.
Registering for this event will provide you to 24/7 access to the webinar for future reference. In addition, a live chat forum will be ongoing throughout the event, should you have any application-specific questions. This webinar is provided completely free as a thank you for your continued support! You can register now at http://www.waters.com/waters/eventInstance.htm?eiid=134740552 or at http://www.waters.com/meettheexperts.